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Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement.

Xu R, Xia H, He W, Li Z, Zhao J, Liu B, Wang Y, Lei Q, Kong Y, Bai Y, Yao Z, Yan R, Li H, Zhan R, Yang S, Luo G, Wu J - Sci Rep (2016)

Bottom Line: Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies.It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model.Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model.

View Article: PubMed Central - PubMed

Affiliation: Institute of Burn Research, Southwest Hospital; State Key Lab of Trauma, Burn and Combined Injury; Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China.

ABSTRACT
A desirable microenvironment is essential for wound healing, in which an ideal moisture content is one of the most important factors. The fundamental function and requirement for wound dressings is to keep the wound at an optimal moisture. Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies. It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model. Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model. Our finds may be helpful in the design of wound dressing for wound regeneration in the future.

No MeSH data available.


Related in: MedlinePlus

Proliferation of keratinocytes in the wound.(a) PCNA immunohistochemical staining in the wound tissue at day 3 and day 7 post-surgery. (b) PCNA and β-actin protein levels were determined by Western blot, and (c) relative densities of PCNA protein level in each group are shown. The values were calculated as the mean ± SD (n = 3), **p < 0.01, *p < 0.05. (d) EGF concentration in the wound exudates. The concentration of EGF in the MP group was much higher than that in other groups. The values were calculated as the mean ± SD (n = 3), **p < 0.01.
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f8: Proliferation of keratinocytes in the wound.(a) PCNA immunohistochemical staining in the wound tissue at day 3 and day 7 post-surgery. (b) PCNA and β-actin protein levels were determined by Western blot, and (c) relative densities of PCNA protein level in each group are shown. The values were calculated as the mean ± SD (n = 3), **p < 0.01, *p < 0.05. (d) EGF concentration in the wound exudates. The concentration of EGF in the MP group was much higher than that in other groups. The values were calculated as the mean ± SD (n = 3), **p < 0.01.

Mentions: Enhanced re-epithelialization might result from the keratinocyte proliferation or the keratinocyte migration. Thus, we first investigated the keratinocyte proliferation at the wound edge. PCNA expression in the epidermis was detected by immunohistochemical staining (Fig. 8a), and it was observed that the intensity of PCNA staining at the wound edge in MP group was higher than that in the other groups at day 3. Western blot results also indicated that the protein level of PCNA in the MP group was significantly higher than that in the other groups. At day 7, we still observed that PCNA expression in the MP group was significantly increased compared with the blank, EHP, LP and ELP groups (Fig. 8b,c). Meanwhile, the EGF concentration of the wound exudates was determined, and it was observed that the concentration of EGF in the MP group was much higher than that of the other groups (Fig. 8d).


Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement.

Xu R, Xia H, He W, Li Z, Zhao J, Liu B, Wang Y, Lei Q, Kong Y, Bai Y, Yao Z, Yan R, Li H, Zhan R, Yang S, Luo G, Wu J - Sci Rep (2016)

Proliferation of keratinocytes in the wound.(a) PCNA immunohistochemical staining in the wound tissue at day 3 and day 7 post-surgery. (b) PCNA and β-actin protein levels were determined by Western blot, and (c) relative densities of PCNA protein level in each group are shown. The values were calculated as the mean ± SD (n = 3), **p < 0.01, *p < 0.05. (d) EGF concentration in the wound exudates. The concentration of EGF in the MP group was much higher than that in other groups. The values were calculated as the mean ± SD (n = 3), **p < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834567&req=5

f8: Proliferation of keratinocytes in the wound.(a) PCNA immunohistochemical staining in the wound tissue at day 3 and day 7 post-surgery. (b) PCNA and β-actin protein levels were determined by Western blot, and (c) relative densities of PCNA protein level in each group are shown. The values were calculated as the mean ± SD (n = 3), **p < 0.01, *p < 0.05. (d) EGF concentration in the wound exudates. The concentration of EGF in the MP group was much higher than that in other groups. The values were calculated as the mean ± SD (n = 3), **p < 0.01.
Mentions: Enhanced re-epithelialization might result from the keratinocyte proliferation or the keratinocyte migration. Thus, we first investigated the keratinocyte proliferation at the wound edge. PCNA expression in the epidermis was detected by immunohistochemical staining (Fig. 8a), and it was observed that the intensity of PCNA staining at the wound edge in MP group was higher than that in the other groups at day 3. Western blot results also indicated that the protein level of PCNA in the MP group was significantly higher than that in the other groups. At day 7, we still observed that PCNA expression in the MP group was significantly increased compared with the blank, EHP, LP and ELP groups (Fig. 8b,c). Meanwhile, the EGF concentration of the wound exudates was determined, and it was observed that the concentration of EGF in the MP group was much higher than that of the other groups (Fig. 8d).

Bottom Line: Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies.It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model.Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model.

View Article: PubMed Central - PubMed

Affiliation: Institute of Burn Research, Southwest Hospital; State Key Lab of Trauma, Burn and Combined Injury; Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China.

ABSTRACT
A desirable microenvironment is essential for wound healing, in which an ideal moisture content is one of the most important factors. The fundamental function and requirement for wound dressings is to keep the wound at an optimal moisture. Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies. It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model. Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model. Our finds may be helpful in the design of wound dressing for wound regeneration in the future.

No MeSH data available.


Related in: MedlinePlus