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Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement.

Xu R, Xia H, He W, Li Z, Zhao J, Liu B, Wang Y, Lei Q, Kong Y, Bai Y, Yao Z, Yan R, Li H, Zhan R, Yang S, Luo G, Wu J - Sci Rep (2016)

Bottom Line: Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies.It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model.Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model.

View Article: PubMed Central - PubMed

Affiliation: Institute of Burn Research, Southwest Hospital; State Key Lab of Trauma, Burn and Combined Injury; Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China.

ABSTRACT
A desirable microenvironment is essential for wound healing, in which an ideal moisture content is one of the most important factors. The fundamental function and requirement for wound dressings is to keep the wound at an optimal moisture. Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies. It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model. Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model. Our finds may be helpful in the design of wound dressing for wound regeneration in the future.

No MeSH data available.


Related in: MedlinePlus

Re-epithelialization of the wound.(a) The newly formed epithelium in the wound tissue at 3 and 7 days post-wounding and (b) the length of the newly formed epithelium was significantly increased when MP-PU membrane was applied. The values were calculated as the mean ± SD (n = 5), **p < 0.01, *p < 0.05.
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f7: Re-epithelialization of the wound.(a) The newly formed epithelium in the wound tissue at 3 and 7 days post-wounding and (b) the length of the newly formed epithelium was significantly increased when MP-PU membrane was applied. The values were calculated as the mean ± SD (n = 5), **p < 0.01, *p < 0.05.

Mentions: The length of the neo-epithelium was measured based on the HE staining sections. At days 3 and 7, histological analysis revealed that the average length of the neo-epithelium in MP group was 392.4 μm and 1450.6 μm, respectively; these values were significantly greater than those observed for the blank, EHP, HP, LP and ELP groups (Fig. 7).


Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement.

Xu R, Xia H, He W, Li Z, Zhao J, Liu B, Wang Y, Lei Q, Kong Y, Bai Y, Yao Z, Yan R, Li H, Zhan R, Yang S, Luo G, Wu J - Sci Rep (2016)

Re-epithelialization of the wound.(a) The newly formed epithelium in the wound tissue at 3 and 7 days post-wounding and (b) the length of the newly formed epithelium was significantly increased when MP-PU membrane was applied. The values were calculated as the mean ± SD (n = 5), **p < 0.01, *p < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834567&req=5

f7: Re-epithelialization of the wound.(a) The newly formed epithelium in the wound tissue at 3 and 7 days post-wounding and (b) the length of the newly formed epithelium was significantly increased when MP-PU membrane was applied. The values were calculated as the mean ± SD (n = 5), **p < 0.01, *p < 0.05.
Mentions: The length of the neo-epithelium was measured based on the HE staining sections. At days 3 and 7, histological analysis revealed that the average length of the neo-epithelium in MP group was 392.4 μm and 1450.6 μm, respectively; these values were significantly greater than those observed for the blank, EHP, HP, LP and ELP groups (Fig. 7).

Bottom Line: Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies.It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model.Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model.

View Article: PubMed Central - PubMed

Affiliation: Institute of Burn Research, Southwest Hospital; State Key Lab of Trauma, Burn and Combined Injury; Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China.

ABSTRACT
A desirable microenvironment is essential for wound healing, in which an ideal moisture content is one of the most important factors. The fundamental function and requirement for wound dressings is to keep the wound at an optimal moisture. Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies. It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model. Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model. Our finds may be helpful in the design of wound dressing for wound regeneration in the future.

No MeSH data available.


Related in: MedlinePlus