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Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement.

Xu R, Xia H, He W, Li Z, Zhao J, Liu B, Wang Y, Lei Q, Kong Y, Bai Y, Yao Z, Yan R, Li H, Zhan R, Yang S, Luo G, Wu J - Sci Rep (2016)

Bottom Line: Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies.It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model.Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model.

View Article: PubMed Central - PubMed

Affiliation: Institute of Burn Research, Southwest Hospital; State Key Lab of Trauma, Burn and Combined Injury; Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China.

ABSTRACT
A desirable microenvironment is essential for wound healing, in which an ideal moisture content is one of the most important factors. The fundamental function and requirement for wound dressings is to keep the wound at an optimal moisture. Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies. It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model. Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model. Our finds may be helpful in the design of wound dressing for wound regeneration in the future.

No MeSH data available.


Related in: MedlinePlus

Expression of α-SMA in the wound tissue.(a) α-SMA immunohistochemical staining in the wound tissue at 7 days post-surgery. (b) α-SMA and β-actin protein levels were determined by Western blot, and (c) relative densities of α-SMA protein level in each group are shown. The values were calculated as the mean ± SD (n = 3), **p < 0.01, *p < 0.05.
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f6: Expression of α-SMA in the wound tissue.(a) α-SMA immunohistochemical staining in the wound tissue at 7 days post-surgery. (b) α-SMA and β-actin protein levels were determined by Western blot, and (c) relative densities of α-SMA protein level in each group are shown. The values were calculated as the mean ± SD (n = 3), **p < 0.01, *p < 0.05.

Mentions: Secondly, α-SMA expression in wound tissue was examined by both immunohistochemical staining (Fig. 6a) and western blotting (Fig. 6b). It was revealed that α-SMA expression was significantly enhanced in MP group compared to blank, EHP, LP and ELP groups at the protein level (Fig. 6c).


Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement.

Xu R, Xia H, He W, Li Z, Zhao J, Liu B, Wang Y, Lei Q, Kong Y, Bai Y, Yao Z, Yan R, Li H, Zhan R, Yang S, Luo G, Wu J - Sci Rep (2016)

Expression of α-SMA in the wound tissue.(a) α-SMA immunohistochemical staining in the wound tissue at 7 days post-surgery. (b) α-SMA and β-actin protein levels were determined by Western blot, and (c) relative densities of α-SMA protein level in each group are shown. The values were calculated as the mean ± SD (n = 3), **p < 0.01, *p < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834567&req=5

f6: Expression of α-SMA in the wound tissue.(a) α-SMA immunohistochemical staining in the wound tissue at 7 days post-surgery. (b) α-SMA and β-actin protein levels were determined by Western blot, and (c) relative densities of α-SMA protein level in each group are shown. The values were calculated as the mean ± SD (n = 3), **p < 0.01, *p < 0.05.
Mentions: Secondly, α-SMA expression in wound tissue was examined by both immunohistochemical staining (Fig. 6a) and western blotting (Fig. 6b). It was revealed that α-SMA expression was significantly enhanced in MP group compared to blank, EHP, LP and ELP groups at the protein level (Fig. 6c).

Bottom Line: Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies.It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model.Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model.

View Article: PubMed Central - PubMed

Affiliation: Institute of Burn Research, Southwest Hospital; State Key Lab of Trauma, Burn and Combined Injury; Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China.

ABSTRACT
A desirable microenvironment is essential for wound healing, in which an ideal moisture content is one of the most important factors. The fundamental function and requirement for wound dressings is to keep the wound at an optimal moisture. Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies. It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model. Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model. Our finds may be helpful in the design of wound dressing for wound regeneration in the future.

No MeSH data available.


Related in: MedlinePlus