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Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement.

Xu R, Xia H, He W, Li Z, Zhao J, Liu B, Wang Y, Lei Q, Kong Y, Bai Y, Yao Z, Yan R, Li H, Zhan R, Yang S, Luo G, Wu J - Sci Rep (2016)

Bottom Line: Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies.It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model.Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model.

View Article: PubMed Central - PubMed

Affiliation: Institute of Burn Research, Southwest Hospital; State Key Lab of Trauma, Burn and Combined Injury; Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China.

ABSTRACT
A desirable microenvironment is essential for wound healing, in which an ideal moisture content is one of the most important factors. The fundamental function and requirement for wound dressings is to keep the wound at an optimal moisture. Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies. It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model. Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model. Our finds may be helpful in the design of wound dressing for wound regeneration in the future.

No MeSH data available.


Related in: MedlinePlus

Formation of granulation tissue.(a) H&E staining of the wound tissue at 3 and 7 days post-wounding and (b) Thickness of granulation tissue. The average thickness of granulation tissue in the MP group was much thicker than that in other groups. The values were calculated as the mean ± SD (n = 5), **p < 0.01, *p < 0.05.
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f5: Formation of granulation tissue.(a) H&E staining of the wound tissue at 3 and 7 days post-wounding and (b) Thickness of granulation tissue. The average thickness of granulation tissue in the MP group was much thicker than that in other groups. The values were calculated as the mean ± SD (n = 5), **p < 0.01, *p < 0.05.

Mentions: To understand the possible mechanism of the enhanced wound contraction, firstly, HE staining of the wound tissue was performed to evaluate the amount of the newly regenerated granulation tissue (Fig. 5a). As presented in Fig. 5b, on the 7th day, the average thicknesses of the wound granulation tissue covered with the MP-PU membrane was 780.1 μm, which was much thicker than that in blank, EHP, HP, LP and ELP groups (252.7 μm, 353.5 μm, 416.2 μm, 310.9 μm and 102.4 μm, respectively; Fig. 5b).


Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement.

Xu R, Xia H, He W, Li Z, Zhao J, Liu B, Wang Y, Lei Q, Kong Y, Bai Y, Yao Z, Yan R, Li H, Zhan R, Yang S, Luo G, Wu J - Sci Rep (2016)

Formation of granulation tissue.(a) H&E staining of the wound tissue at 3 and 7 days post-wounding and (b) Thickness of granulation tissue. The average thickness of granulation tissue in the MP group was much thicker than that in other groups. The values were calculated as the mean ± SD (n = 5), **p < 0.01, *p < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834567&req=5

f5: Formation of granulation tissue.(a) H&E staining of the wound tissue at 3 and 7 days post-wounding and (b) Thickness of granulation tissue. The average thickness of granulation tissue in the MP group was much thicker than that in other groups. The values were calculated as the mean ± SD (n = 5), **p < 0.01, *p < 0.05.
Mentions: To understand the possible mechanism of the enhanced wound contraction, firstly, HE staining of the wound tissue was performed to evaluate the amount of the newly regenerated granulation tissue (Fig. 5a). As presented in Fig. 5b, on the 7th day, the average thicknesses of the wound granulation tissue covered with the MP-PU membrane was 780.1 μm, which was much thicker than that in blank, EHP, HP, LP and ELP groups (252.7 μm, 353.5 μm, 416.2 μm, 310.9 μm and 102.4 μm, respectively; Fig. 5b).

Bottom Line: Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies.It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model.Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model.

View Article: PubMed Central - PubMed

Affiliation: Institute of Burn Research, Southwest Hospital; State Key Lab of Trauma, Burn and Combined Injury; Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China.

ABSTRACT
A desirable microenvironment is essential for wound healing, in which an ideal moisture content is one of the most important factors. The fundamental function and requirement for wound dressings is to keep the wound at an optimal moisture. Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies. It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model. Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model. Our finds may be helpful in the design of wound dressing for wound regeneration in the future.

No MeSH data available.


Related in: MedlinePlus