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Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement.

Xu R, Xia H, He W, Li Z, Zhao J, Liu B, Wang Y, Lei Q, Kong Y, Bai Y, Yao Z, Yan R, Li H, Zhan R, Yang S, Luo G, Wu J - Sci Rep (2016)

Bottom Line: Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies.It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model.Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model.

View Article: PubMed Central - PubMed

Affiliation: Institute of Burn Research, Southwest Hospital; State Key Lab of Trauma, Burn and Combined Injury; Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China.

ABSTRACT
A desirable microenvironment is essential for wound healing, in which an ideal moisture content is one of the most important factors. The fundamental function and requirement for wound dressings is to keep the wound at an optimal moisture. Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies. It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model. Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model. Our finds may be helpful in the design of wound dressing for wound regeneration in the future.

No MeSH data available.


Related in: MedlinePlus

The MP-PU membrane controlled water loss at a suitable level which enhanced gel contraction and cell proliferation.(a) Residue of the culture medium in the FPCL model, (b) number of fibroblasts, (c) FPCL models for the collagen contraction assay and (d) rate of contraction. The residue of culture medium in the MP-PU group was 0.01 g, and its rate of contraction was larger than that of the HP and ELP groups. The values were calculated as the mean ± SD (n = 5), **p < 0.01, *p < 0.05. (e) Residue of the culture medium in the HaCat three-dimensional culture model and (f) the number of HaCat cells. Cell numbers in the MP group were greater than those in the EHP and the HP groups. The values were calculated as the mean ± SD (n = 5), *p < 0.05.
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f3: The MP-PU membrane controlled water loss at a suitable level which enhanced gel contraction and cell proliferation.(a) Residue of the culture medium in the FPCL model, (b) number of fibroblasts, (c) FPCL models for the collagen contraction assay and (d) rate of contraction. The residue of culture medium in the MP-PU group was 0.01 g, and its rate of contraction was larger than that of the HP and ELP groups. The values were calculated as the mean ± SD (n = 5), **p < 0.01, *p < 0.05. (e) Residue of the culture medium in the HaCat three-dimensional culture model and (f) the number of HaCat cells. Cell numbers in the MP group were greater than those in the EHP and the HP groups. The values were calculated as the mean ± SD (n = 5), *p < 0.05.

Mentions: In the fibroblast three-dimensional culture model, i.e. fibroblast populated collagen lattice (FPCL) model, it was observed that the culture medium in the blank and EHP groups evaporated completely (the culture medium and the gel completely dried out). In contrast, large volumes of culture medium remained in the LP and ELP groups (Fig. 3c). The average culture medium residue was measured to be 0.01 g when the culture dish was covered with MP-PU, and the average rate of contraction was 32.9%, which was larger than those of the HP and ELP groups (4.9% and 9.4%, respectively; Fig. 3a,d). It was also observed that the number of fibroblasts in the MP group was greater than that in the EHP, HP and LP groups (Fig. 3b).


Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement.

Xu R, Xia H, He W, Li Z, Zhao J, Liu B, Wang Y, Lei Q, Kong Y, Bai Y, Yao Z, Yan R, Li H, Zhan R, Yang S, Luo G, Wu J - Sci Rep (2016)

The MP-PU membrane controlled water loss at a suitable level which enhanced gel contraction and cell proliferation.(a) Residue of the culture medium in the FPCL model, (b) number of fibroblasts, (c) FPCL models for the collagen contraction assay and (d) rate of contraction. The residue of culture medium in the MP-PU group was 0.01 g, and its rate of contraction was larger than that of the HP and ELP groups. The values were calculated as the mean ± SD (n = 5), **p < 0.01, *p < 0.05. (e) Residue of the culture medium in the HaCat three-dimensional culture model and (f) the number of HaCat cells. Cell numbers in the MP group were greater than those in the EHP and the HP groups. The values were calculated as the mean ± SD (n = 5), *p < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834567&req=5

f3: The MP-PU membrane controlled water loss at a suitable level which enhanced gel contraction and cell proliferation.(a) Residue of the culture medium in the FPCL model, (b) number of fibroblasts, (c) FPCL models for the collagen contraction assay and (d) rate of contraction. The residue of culture medium in the MP-PU group was 0.01 g, and its rate of contraction was larger than that of the HP and ELP groups. The values were calculated as the mean ± SD (n = 5), **p < 0.01, *p < 0.05. (e) Residue of the culture medium in the HaCat three-dimensional culture model and (f) the number of HaCat cells. Cell numbers in the MP group were greater than those in the EHP and the HP groups. The values were calculated as the mean ± SD (n = 5), *p < 0.05.
Mentions: In the fibroblast three-dimensional culture model, i.e. fibroblast populated collagen lattice (FPCL) model, it was observed that the culture medium in the blank and EHP groups evaporated completely (the culture medium and the gel completely dried out). In contrast, large volumes of culture medium remained in the LP and ELP groups (Fig. 3c). The average culture medium residue was measured to be 0.01 g when the culture dish was covered with MP-PU, and the average rate of contraction was 32.9%, which was larger than those of the HP and ELP groups (4.9% and 9.4%, respectively; Fig. 3a,d). It was also observed that the number of fibroblasts in the MP group was greater than that in the EHP, HP and LP groups (Fig. 3b).

Bottom Line: Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies.It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model.Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model.

View Article: PubMed Central - PubMed

Affiliation: Institute of Burn Research, Southwest Hospital; State Key Lab of Trauma, Burn and Combined Injury; Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China.

ABSTRACT
A desirable microenvironment is essential for wound healing, in which an ideal moisture content is one of the most important factors. The fundamental function and requirement for wound dressings is to keep the wound at an optimal moisture. Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies. It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model. Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model. Our finds may be helpful in the design of wound dressing for wound regeneration in the future.

No MeSH data available.


Related in: MedlinePlus