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Intensity interrogation near cutoff resonance for label-free cellular profiling.

Nazirizadeh Y, Behrends V, Prósz A, Orgovan N, Horvath R, Ferrie AM, Fang Y, Selhuber-Unkel C, Gerken M - Sci Rep (2016)

Bottom Line: We report a method enabling intensity-based readout for label-free cellular assays, and realize a reader device with the same footprint as a microtiter plate.For unambiguous resonance intensity measurements in resonance waveguide grating (RWG) sensors, we propose to apply resonances near the substrate cutoff wavelength.The significantly reduced size of the reader device opens new opportunities for easy integration into incubators or liquid handling systems.

View Article: PubMed Central - PubMed

Affiliation: Byosens GmbH, 20357 Hamburg, Germany.

ABSTRACT
We report a method enabling intensity-based readout for label-free cellular assays, and realize a reader device with the same footprint as a microtiter plate. For unambiguous resonance intensity measurements in resonance waveguide grating (RWG) sensors, we propose to apply resonances near the substrate cutoff wavelength. This method was validated in bulk refractive index, surface bilayer and G protein-coupled receptor (GPCR) experiments. The significantly reduced size of the reader device opens new opportunities for easy integration into incubators or liquid handling systems.

No MeSH data available.


Related in: MedlinePlus

Intensity-based RWG sensor readout.(a) Schematic of the intensity-based readout consisting of a light emitting diode (LED) with collimation optics, photodiode with focusing optics and a circular polarization filter. (b) Demonstrator reader for 96-well microtiter plate (Epic from Corning). The footprint of this reader is the same as the microtiter plate itself. (c) Bulk refractive index experiments with glycerol dilutions in water. (d) Surface bilayer experiments with polyelectrolyte films.
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f2: Intensity-based RWG sensor readout.(a) Schematic of the intensity-based readout consisting of a light emitting diode (LED) with collimation optics, photodiode with focusing optics and a circular polarization filter. (b) Demonstrator reader for 96-well microtiter plate (Epic from Corning). The footprint of this reader is the same as the microtiter plate itself. (c) Bulk refractive index experiments with glycerol dilutions in water. (d) Surface bilayer experiments with polyelectrolyte films.

Mentions: By leveraging this finding, we designed a readout system using the near-cutoff resonance intensity without the need for a spectrometer and charge-coupled device. The readout components are reduced to a light emitting diode (LED) with a collimation optics, a photodiode with a focusing optics and a circular polarization filter to filter out parasitic reflections from the sensor downside (Fig. 2a). This component and size reduction enables a label-free microtiter plate reader with 96 readout units integrated onto a printed circuit board (PCB). The reader has the same footprint as the microtiter plate itself (Fig. 2b). The response of this reader to surface mass change was investigated in a bulk refractive index experiment with glycerol solutions, and in a surface layer-by-layer experiment with negatively charged poly(sodium 4-styrenesul- fonate) (PSS) and positively charged poly (allylamine hydrochloride) (PAH)9. The employed solutions were crosschecked with a refractometer. The first experiment, imitates a bulk mass change occurring in proliferation assays, while the second experiment imitates the surface mass change as found in DMR experiments. Both experiments showed linear responses to the mass changes (Fig. 2c,d, respectively).


Intensity interrogation near cutoff resonance for label-free cellular profiling.

Nazirizadeh Y, Behrends V, Prósz A, Orgovan N, Horvath R, Ferrie AM, Fang Y, Selhuber-Unkel C, Gerken M - Sci Rep (2016)

Intensity-based RWG sensor readout.(a) Schematic of the intensity-based readout consisting of a light emitting diode (LED) with collimation optics, photodiode with focusing optics and a circular polarization filter. (b) Demonstrator reader for 96-well microtiter plate (Epic from Corning). The footprint of this reader is the same as the microtiter plate itself. (c) Bulk refractive index experiments with glycerol dilutions in water. (d) Surface bilayer experiments with polyelectrolyte films.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834563&req=5

f2: Intensity-based RWG sensor readout.(a) Schematic of the intensity-based readout consisting of a light emitting diode (LED) with collimation optics, photodiode with focusing optics and a circular polarization filter. (b) Demonstrator reader for 96-well microtiter plate (Epic from Corning). The footprint of this reader is the same as the microtiter plate itself. (c) Bulk refractive index experiments with glycerol dilutions in water. (d) Surface bilayer experiments with polyelectrolyte films.
Mentions: By leveraging this finding, we designed a readout system using the near-cutoff resonance intensity without the need for a spectrometer and charge-coupled device. The readout components are reduced to a light emitting diode (LED) with a collimation optics, a photodiode with a focusing optics and a circular polarization filter to filter out parasitic reflections from the sensor downside (Fig. 2a). This component and size reduction enables a label-free microtiter plate reader with 96 readout units integrated onto a printed circuit board (PCB). The reader has the same footprint as the microtiter plate itself (Fig. 2b). The response of this reader to surface mass change was investigated in a bulk refractive index experiment with glycerol solutions, and in a surface layer-by-layer experiment with negatively charged poly(sodium 4-styrenesul- fonate) (PSS) and positively charged poly (allylamine hydrochloride) (PAH)9. The employed solutions were crosschecked with a refractometer. The first experiment, imitates a bulk mass change occurring in proliferation assays, while the second experiment imitates the surface mass change as found in DMR experiments. Both experiments showed linear responses to the mass changes (Fig. 2c,d, respectively).

Bottom Line: We report a method enabling intensity-based readout for label-free cellular assays, and realize a reader device with the same footprint as a microtiter plate.For unambiguous resonance intensity measurements in resonance waveguide grating (RWG) sensors, we propose to apply resonances near the substrate cutoff wavelength.The significantly reduced size of the reader device opens new opportunities for easy integration into incubators or liquid handling systems.

View Article: PubMed Central - PubMed

Affiliation: Byosens GmbH, 20357 Hamburg, Germany.

ABSTRACT
We report a method enabling intensity-based readout for label-free cellular assays, and realize a reader device with the same footprint as a microtiter plate. For unambiguous resonance intensity measurements in resonance waveguide grating (RWG) sensors, we propose to apply resonances near the substrate cutoff wavelength. This method was validated in bulk refractive index, surface bilayer and G protein-coupled receptor (GPCR) experiments. The significantly reduced size of the reader device opens new opportunities for easy integration into incubators or liquid handling systems.

No MeSH data available.


Related in: MedlinePlus