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Engineering a predatory bacterium as a proficient killer agent for intracellular bio-products recovery: The case of the polyhydroxyalkanoates.

Martínez V, Herencias C, Jurkevitch E, Prieto MA - Sci Rep (2016)

Bottom Line: This work examines the potential of the predatory bacterium Bdellovibrio bacteriovorus HD100, an obligate predator of other Gram-negative bacteria, as an external cell-lytic agent for recovering valuable intracellular bio-products produced by prey cultures.The bio-product targets to be recovered were polyhydroxyalkanoates (PHAs) produced naturally by Pseudomonas putida and Cupriavidus necator, or by recombinant Escherichia coli strains.B. bacteriovorus with a mutated PHA depolymerase gene to prevent the unwanted breakdown of the bio-product allowed the recovery of up to 80% of that accumulated by the prey bacteria, even at high biomass concentrations.

View Article: PubMed Central - PubMed

Affiliation: Environmental Biology Department, Centro de Investigaciones Biológicas, CSIC, C/Ramiro de Maeztu 9, 28040 Madrid, Spain.

ABSTRACT
This work examines the potential of the predatory bacterium Bdellovibrio bacteriovorus HD100, an obligate predator of other Gram-negative bacteria, as an external cell-lytic agent for recovering valuable intracellular bio-products produced by prey cultures. The bio-product targets to be recovered were polyhydroxyalkanoates (PHAs) produced naturally by Pseudomonas putida and Cupriavidus necator, or by recombinant Escherichia coli strains. B. bacteriovorus with a mutated PHA depolymerase gene to prevent the unwanted breakdown of the bio-product allowed the recovery of up to 80% of that accumulated by the prey bacteria, even at high biomass concentrations. This innovative downstream process highlights how B. bacteriovorus can be used as a novel, biological lytic agent for the inexpensive, industrial scale recovery of intracellular products from different Gram-negative prey cultures.

No MeSH data available.


Related in: MedlinePlus

Illustration of the lytic system procedure based on the use of B. bacteriovorus for intracellular bio-products recovery.A culture of PHA-producing bacteria is prepared and infected with a suspension of B. bacteriovorus cells. After 24 h of predation the intracellular bio-product is released into the culture medium, facilitating the recovery.
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f2: Illustration of the lytic system procedure based on the use of B. bacteriovorus for intracellular bio-products recovery.A culture of PHA-producing bacteria is prepared and infected with a suspension of B. bacteriovorus cells. After 24 h of predation the intracellular bio-product is released into the culture medium, facilitating the recovery.

Mentions: Bdellovibrio bacteriovorus is a small, highly motile, predatory bacterium that attacks other Gram-negative bacteria, invading their periplasm12. Once inside, the enzymatic degradation of prey constituents is initiated and the invader begins to grow, leading to the formation of a bdelloplast. The predator grows as a multinucleoid filament that finally septates to yield several progeny that escape the prey ghost to search for new prey cell (Fig. 1a–d). B. bacteriovorus was originally discovered in soil samples3 but has now been isolated from many environments, ranging from marine sediments to fresh water and even the guts of animals and humans4567. This, together with an aptitude for preying on biofilms and multidrug-resistant pathogens, makes B. bacteriovorus a potential therapeutic agent for controlling human, animal and plant pathogens, the so-called “living antibiotic”289101112. In this work, we evaluated the potential use of a killer bacterium like B. bacteriovorus for biotechnological purposes. Given the predatory lifestyle of Bdellovibrio and its ability to lyse other bacteria, we investigated the feasibility for exploiting this predator as a novel downstream living lytic agent for the production of valuable intracellular bio-products (Fig. 2).


Engineering a predatory bacterium as a proficient killer agent for intracellular bio-products recovery: The case of the polyhydroxyalkanoates.

Martínez V, Herencias C, Jurkevitch E, Prieto MA - Sci Rep (2016)

Illustration of the lytic system procedure based on the use of B. bacteriovorus for intracellular bio-products recovery.A culture of PHA-producing bacteria is prepared and infected with a suspension of B. bacteriovorus cells. After 24 h of predation the intracellular bio-product is released into the culture medium, facilitating the recovery.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834554&req=5

f2: Illustration of the lytic system procedure based on the use of B. bacteriovorus for intracellular bio-products recovery.A culture of PHA-producing bacteria is prepared and infected with a suspension of B. bacteriovorus cells. After 24 h of predation the intracellular bio-product is released into the culture medium, facilitating the recovery.
Mentions: Bdellovibrio bacteriovorus is a small, highly motile, predatory bacterium that attacks other Gram-negative bacteria, invading their periplasm12. Once inside, the enzymatic degradation of prey constituents is initiated and the invader begins to grow, leading to the formation of a bdelloplast. The predator grows as a multinucleoid filament that finally septates to yield several progeny that escape the prey ghost to search for new prey cell (Fig. 1a–d). B. bacteriovorus was originally discovered in soil samples3 but has now been isolated from many environments, ranging from marine sediments to fresh water and even the guts of animals and humans4567. This, together with an aptitude for preying on biofilms and multidrug-resistant pathogens, makes B. bacteriovorus a potential therapeutic agent for controlling human, animal and plant pathogens, the so-called “living antibiotic”289101112. In this work, we evaluated the potential use of a killer bacterium like B. bacteriovorus for biotechnological purposes. Given the predatory lifestyle of Bdellovibrio and its ability to lyse other bacteria, we investigated the feasibility for exploiting this predator as a novel downstream living lytic agent for the production of valuable intracellular bio-products (Fig. 2).

Bottom Line: This work examines the potential of the predatory bacterium Bdellovibrio bacteriovorus HD100, an obligate predator of other Gram-negative bacteria, as an external cell-lytic agent for recovering valuable intracellular bio-products produced by prey cultures.The bio-product targets to be recovered were polyhydroxyalkanoates (PHAs) produced naturally by Pseudomonas putida and Cupriavidus necator, or by recombinant Escherichia coli strains.B. bacteriovorus with a mutated PHA depolymerase gene to prevent the unwanted breakdown of the bio-product allowed the recovery of up to 80% of that accumulated by the prey bacteria, even at high biomass concentrations.

View Article: PubMed Central - PubMed

Affiliation: Environmental Biology Department, Centro de Investigaciones Biológicas, CSIC, C/Ramiro de Maeztu 9, 28040 Madrid, Spain.

ABSTRACT
This work examines the potential of the predatory bacterium Bdellovibrio bacteriovorus HD100, an obligate predator of other Gram-negative bacteria, as an external cell-lytic agent for recovering valuable intracellular bio-products produced by prey cultures. The bio-product targets to be recovered were polyhydroxyalkanoates (PHAs) produced naturally by Pseudomonas putida and Cupriavidus necator, or by recombinant Escherichia coli strains. B. bacteriovorus with a mutated PHA depolymerase gene to prevent the unwanted breakdown of the bio-product allowed the recovery of up to 80% of that accumulated by the prey bacteria, even at high biomass concentrations. This innovative downstream process highlights how B. bacteriovorus can be used as a novel, biological lytic agent for the inexpensive, industrial scale recovery of intracellular products from different Gram-negative prey cultures.

No MeSH data available.


Related in: MedlinePlus