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Crypt cells are involved in kin recognition in larval zebrafish.

Biechl D, Tietje K, Gerlach G, Wullimann MF - Sci Rep (2016)

Bottom Line: Zebrafish larvae imprint on visual and olfactory kin cues at day 5 and 6 postfertilization, respectively, resulting in kin recognition later in life.Then, we tested imprinted and non-imprinted larvae (full siblings) for kin odor detection.We provide the first direct evidence that crypt cells, and likely a subpopulation of microvillous OSNs, but not ciliated OSNs, play a role in detecting a kin odor related signal.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Systemic Neurosciences &Department Biology II, Ludwig-Maximilians-Universität Munich, Grosshadernerstr. 2, 82152 Planegg-Martinsried Germany.

ABSTRACT
Zebrafish larvae imprint on visual and olfactory kin cues at day 5 and 6 postfertilization, respectively, resulting in kin recognition later in life. Exposure to non-kin cues prevents imprinting and kin recognition. Imprinting depends on MHC class II related signals and only larvae sharing MHC class II alleles can imprint on each other. Here, we analyzed which type of olfactory sensory neuron (OSN) detects kin odor. The single teleost olfactory epithelium harbors ciliated OSNs carrying OR and TAAR gene family receptors (mammals: main olfactory epithelium) and microvillous OSNs with V1R and V2R gene family receptors (mammals: vomeronasal organ). Additionally, teleosts exhibit crypt cells which possess microvilli and cilia. We used the activity marker pERK (phosphorylated extracellular signal regulated kinase) after stimulating 9 day old zebrafish larvae with either non-kin conspecific or food odor. While food odor activated both ciliated and microvillous OSNs, only the latter were activated by conspecific odor, crypt cells showed no activation to both stimuli. Then, we tested imprinted and non-imprinted larvae (full siblings) for kin odor detection. We provide the first direct evidence that crypt cells, and likely a subpopulation of microvillous OSNs, but not ciliated OSNs, play a role in detecting a kin odor related signal.

No MeSH data available.


Related in: MedlinePlus

Schemes show three setups of performed experiments.(a) Validation of pERK as a marker for olfactory sensory neuron activity. (b) Kin odor test I. (c) Kin odor test II.
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f1: Schemes show three setups of performed experiments.(a) Validation of pERK as a marker for olfactory sensory neuron activity. (b) Kin odor test I. (c) Kin odor test II.

Mentions: To validate pERK as a neuronal activity marker in the larval zebrafish olfactory epithelium, we performed a temporal analysis of pERK upregulation with two different olfactory stimuli in comparison to control stimulation with E3 medium. This experiment should give information on best duration of stimulation and additionally on the question whether pERK immunofluorescence shows different activation patterns due to different stimuli. We stimulated 9 day old larvae (group reared; see Methods and Fig. 1a) with either non-kin conspecific larvae odor, food odor or, for controls, with E3 medium for 3, 7, 11 and 15 minutes. Afterwards, we used an antibody against pERK to mark activated OSNs within the olfactory epithelium and counted those OSNs using the accepted morphological criteria for the different types of OSNs (see Methods).


Crypt cells are involved in kin recognition in larval zebrafish.

Biechl D, Tietje K, Gerlach G, Wullimann MF - Sci Rep (2016)

Schemes show three setups of performed experiments.(a) Validation of pERK as a marker for olfactory sensory neuron activity. (b) Kin odor test I. (c) Kin odor test II.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834543&req=5

f1: Schemes show three setups of performed experiments.(a) Validation of pERK as a marker for olfactory sensory neuron activity. (b) Kin odor test I. (c) Kin odor test II.
Mentions: To validate pERK as a neuronal activity marker in the larval zebrafish olfactory epithelium, we performed a temporal analysis of pERK upregulation with two different olfactory stimuli in comparison to control stimulation with E3 medium. This experiment should give information on best duration of stimulation and additionally on the question whether pERK immunofluorescence shows different activation patterns due to different stimuli. We stimulated 9 day old larvae (group reared; see Methods and Fig. 1a) with either non-kin conspecific larvae odor, food odor or, for controls, with E3 medium for 3, 7, 11 and 15 minutes. Afterwards, we used an antibody against pERK to mark activated OSNs within the olfactory epithelium and counted those OSNs using the accepted morphological criteria for the different types of OSNs (see Methods).

Bottom Line: Zebrafish larvae imprint on visual and olfactory kin cues at day 5 and 6 postfertilization, respectively, resulting in kin recognition later in life.Then, we tested imprinted and non-imprinted larvae (full siblings) for kin odor detection.We provide the first direct evidence that crypt cells, and likely a subpopulation of microvillous OSNs, but not ciliated OSNs, play a role in detecting a kin odor related signal.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Systemic Neurosciences &Department Biology II, Ludwig-Maximilians-Universität Munich, Grosshadernerstr. 2, 82152 Planegg-Martinsried Germany.

ABSTRACT
Zebrafish larvae imprint on visual and olfactory kin cues at day 5 and 6 postfertilization, respectively, resulting in kin recognition later in life. Exposure to non-kin cues prevents imprinting and kin recognition. Imprinting depends on MHC class II related signals and only larvae sharing MHC class II alleles can imprint on each other. Here, we analyzed which type of olfactory sensory neuron (OSN) detects kin odor. The single teleost olfactory epithelium harbors ciliated OSNs carrying OR and TAAR gene family receptors (mammals: main olfactory epithelium) and microvillous OSNs with V1R and V2R gene family receptors (mammals: vomeronasal organ). Additionally, teleosts exhibit crypt cells which possess microvilli and cilia. We used the activity marker pERK (phosphorylated extracellular signal regulated kinase) after stimulating 9 day old zebrafish larvae with either non-kin conspecific or food odor. While food odor activated both ciliated and microvillous OSNs, only the latter were activated by conspecific odor, crypt cells showed no activation to both stimuli. Then, we tested imprinted and non-imprinted larvae (full siblings) for kin odor detection. We provide the first direct evidence that crypt cells, and likely a subpopulation of microvillous OSNs, but not ciliated OSNs, play a role in detecting a kin odor related signal.

No MeSH data available.


Related in: MedlinePlus