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Sensitive Electrochemical Immunosensor for Detection of Nuclear Matrix Protein-22 based on NH2-SAPO-34 Supported Pd/Co Nanoparticles.

Wu D, Wang Y, Zhang Y, Ma H, Yan T, Du B, Wei Q - Sci Rep (2016)

Bottom Line: The reduced graphene oxide-NH (rGO-NH) with good conductivity and large surface area was used to immobilize primary antibody (Ab1).Good reproducibility and stability have showed satisfying results in the analysis of clinical urine samples.This novel and ultrasensitive immunosensor may have the potential application in the detection of different tumor markers.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.

ABSTRACT
A novel sandwich-type electrochemical immunosensor using the new amino group functionalized silicoaluminophosphates molecular sieves (NH2-SAPO-34) supported Pd/Co nanoparticles (NH2-SAPO-34-Pd/Co NPs) as labels for the detection of bladder cancer biomarker nuclear matrix protein-22 (NMP-22) was developed in this work. The reduced graphene oxide-NH (rGO-NH) with good conductivity and large surface area was used to immobilize primary antibody (Ab1). Due to the excellent catalytic activity toward hydrogen peroxide, NH2-SAPO-34-Pd/Co NPs were used as labels and immobilized secondary antibody (Ab2) through adsorption capacity of Pd/Co NPs to protein. The immunosensor displayed a wide linear range (0.001-20 ng/mL) and low detection limit (0.33 pg/mL). Good reproducibility and stability have showed satisfying results in the analysis of clinical urine samples. This novel and ultrasensitive immunosensor may have the potential application in the detection of different tumor markers.

No MeSH data available.


Related in: MedlinePlus

(a) Effect of pH (CrGO-NH = 1.5 mg/mL, pH = 5.85, 6.50, 7.00, 7.40 and 7.89) and (b) the concentration of rGO-NH (pH = 7.0, CrGO-NH = 0.5, 0.8, 1.0, 1.5 and 2.0 mg/mL) on the response of the immunosensor to 10 ng/mL NMP-22. Error bar = RSD (n = 5).
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f5: (a) Effect of pH (CrGO-NH = 1.5 mg/mL, pH = 5.85, 6.50, 7.00, 7.40 and 7.89) and (b) the concentration of rGO-NH (pH = 7.0, CrGO-NH = 0.5, 0.8, 1.0, 1.5 and 2.0 mg/mL) on the response of the immunosensor to 10 ng/mL NMP-22. Error bar = RSD (n = 5).

Mentions: To achieve an optimal electrochemical signal, the influence of the pH value of substrate solution to the immunosensor was investigated at first. Herein, 6.0 μL of rGO-NH solution (1.5 mg/mL) dispersed in chitosan (0.1 wt%) was dropped onto the electrode surface. Then the same amount of Ab1 (6.0 μL, 10 μg/mL) and NMP-22 (6.0 μL, 10 ng/mL) were used to fabricate the immunosensors. As shown in Fig. 5(a), it could be found that the current response increased with increasing pH value from 5.8 to 7.0 to reach the maximum and decreased from 7.0 to 7.9. The reason is that the highly acidic or alkaline surroundings would damage the activity of immobilized protein42. Therefore, pH 7.0 PBS was selected for the test throughout this study.


Sensitive Electrochemical Immunosensor for Detection of Nuclear Matrix Protein-22 based on NH2-SAPO-34 Supported Pd/Co Nanoparticles.

Wu D, Wang Y, Zhang Y, Ma H, Yan T, Du B, Wei Q - Sci Rep (2016)

(a) Effect of pH (CrGO-NH = 1.5 mg/mL, pH = 5.85, 6.50, 7.00, 7.40 and 7.89) and (b) the concentration of rGO-NH (pH = 7.0, CrGO-NH = 0.5, 0.8, 1.0, 1.5 and 2.0 mg/mL) on the response of the immunosensor to 10 ng/mL NMP-22. Error bar = RSD (n = 5).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834490&req=5

f5: (a) Effect of pH (CrGO-NH = 1.5 mg/mL, pH = 5.85, 6.50, 7.00, 7.40 and 7.89) and (b) the concentration of rGO-NH (pH = 7.0, CrGO-NH = 0.5, 0.8, 1.0, 1.5 and 2.0 mg/mL) on the response of the immunosensor to 10 ng/mL NMP-22. Error bar = RSD (n = 5).
Mentions: To achieve an optimal electrochemical signal, the influence of the pH value of substrate solution to the immunosensor was investigated at first. Herein, 6.0 μL of rGO-NH solution (1.5 mg/mL) dispersed in chitosan (0.1 wt%) was dropped onto the electrode surface. Then the same amount of Ab1 (6.0 μL, 10 μg/mL) and NMP-22 (6.0 μL, 10 ng/mL) were used to fabricate the immunosensors. As shown in Fig. 5(a), it could be found that the current response increased with increasing pH value from 5.8 to 7.0 to reach the maximum and decreased from 7.0 to 7.9. The reason is that the highly acidic or alkaline surroundings would damage the activity of immobilized protein42. Therefore, pH 7.0 PBS was selected for the test throughout this study.

Bottom Line: The reduced graphene oxide-NH (rGO-NH) with good conductivity and large surface area was used to immobilize primary antibody (Ab1).Good reproducibility and stability have showed satisfying results in the analysis of clinical urine samples.This novel and ultrasensitive immunosensor may have the potential application in the detection of different tumor markers.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.

ABSTRACT
A novel sandwich-type electrochemical immunosensor using the new amino group functionalized silicoaluminophosphates molecular sieves (NH2-SAPO-34) supported Pd/Co nanoparticles (NH2-SAPO-34-Pd/Co NPs) as labels for the detection of bladder cancer biomarker nuclear matrix protein-22 (NMP-22) was developed in this work. The reduced graphene oxide-NH (rGO-NH) with good conductivity and large surface area was used to immobilize primary antibody (Ab1). Due to the excellent catalytic activity toward hydrogen peroxide, NH2-SAPO-34-Pd/Co NPs were used as labels and immobilized secondary antibody (Ab2) through adsorption capacity of Pd/Co NPs to protein. The immunosensor displayed a wide linear range (0.001-20 ng/mL) and low detection limit (0.33 pg/mL). Good reproducibility and stability have showed satisfying results in the analysis of clinical urine samples. This novel and ultrasensitive immunosensor may have the potential application in the detection of different tumor markers.

No MeSH data available.


Related in: MedlinePlus