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Sensitive Electrochemical Immunosensor for Detection of Nuclear Matrix Protein-22 based on NH2-SAPO-34 Supported Pd/Co Nanoparticles.

Wu D, Wang Y, Zhang Y, Ma H, Yan T, Du B, Wei Q - Sci Rep (2016)

Bottom Line: The reduced graphene oxide-NH (rGO-NH) with good conductivity and large surface area was used to immobilize primary antibody (Ab1).Good reproducibility and stability have showed satisfying results in the analysis of clinical urine samples.This novel and ultrasensitive immunosensor may have the potential application in the detection of different tumor markers.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.

ABSTRACT
A novel sandwich-type electrochemical immunosensor using the new amino group functionalized silicoaluminophosphates molecular sieves (NH2-SAPO-34) supported Pd/Co nanoparticles (NH2-SAPO-34-Pd/Co NPs) as labels for the detection of bladder cancer biomarker nuclear matrix protein-22 (NMP-22) was developed in this work. The reduced graphene oxide-NH (rGO-NH) with good conductivity and large surface area was used to immobilize primary antibody (Ab1). Due to the excellent catalytic activity toward hydrogen peroxide, NH2-SAPO-34-Pd/Co NPs were used as labels and immobilized secondary antibody (Ab2) through adsorption capacity of Pd/Co NPs to protein. The immunosensor displayed a wide linear range (0.001-20 ng/mL) and low detection limit (0.33 pg/mL). Good reproducibility and stability have showed satisfying results in the analysis of clinical urine samples. This novel and ultrasensitive immunosensor may have the potential application in the detection of different tumor markers.

No MeSH data available.


Related in: MedlinePlus

Cyclic voltammograms of electrode modified by NH2-SAPO-34-Pd/Co-Ab2 (2 mg/mL) in N2-saturated PBS without (a) and with (b) 5.0 mmol/L H2O2.
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f3: Cyclic voltammograms of electrode modified by NH2-SAPO-34-Pd/Co-Ab2 (2 mg/mL) in N2-saturated PBS without (a) and with (b) 5.0 mmol/L H2O2.

Mentions: The performance of NH2-SAPO-34-Pd/Co toward the H2O2 reduction was investigated (Fig. 3). As shown, the electrode modified by NH2-SAPO-34-Pd/Co-Ab2 exhibited obvious current change toward H2O2. However, the electrode did not appear to be electroactive toward water. The NH2-SAPO-34-Pd/Co NPs showed the ability to promote the reduction of H2O2 and resulted in the generation of electrochemical signals.


Sensitive Electrochemical Immunosensor for Detection of Nuclear Matrix Protein-22 based on NH2-SAPO-34 Supported Pd/Co Nanoparticles.

Wu D, Wang Y, Zhang Y, Ma H, Yan T, Du B, Wei Q - Sci Rep (2016)

Cyclic voltammograms of electrode modified by NH2-SAPO-34-Pd/Co-Ab2 (2 mg/mL) in N2-saturated PBS without (a) and with (b) 5.0 mmol/L H2O2.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834490&req=5

f3: Cyclic voltammograms of electrode modified by NH2-SAPO-34-Pd/Co-Ab2 (2 mg/mL) in N2-saturated PBS without (a) and with (b) 5.0 mmol/L H2O2.
Mentions: The performance of NH2-SAPO-34-Pd/Co toward the H2O2 reduction was investigated (Fig. 3). As shown, the electrode modified by NH2-SAPO-34-Pd/Co-Ab2 exhibited obvious current change toward H2O2. However, the electrode did not appear to be electroactive toward water. The NH2-SAPO-34-Pd/Co NPs showed the ability to promote the reduction of H2O2 and resulted in the generation of electrochemical signals.

Bottom Line: The reduced graphene oxide-NH (rGO-NH) with good conductivity and large surface area was used to immobilize primary antibody (Ab1).Good reproducibility and stability have showed satisfying results in the analysis of clinical urine samples.This novel and ultrasensitive immunosensor may have the potential application in the detection of different tumor markers.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.

ABSTRACT
A novel sandwich-type electrochemical immunosensor using the new amino group functionalized silicoaluminophosphates molecular sieves (NH2-SAPO-34) supported Pd/Co nanoparticles (NH2-SAPO-34-Pd/Co NPs) as labels for the detection of bladder cancer biomarker nuclear matrix protein-22 (NMP-22) was developed in this work. The reduced graphene oxide-NH (rGO-NH) with good conductivity and large surface area was used to immobilize primary antibody (Ab1). Due to the excellent catalytic activity toward hydrogen peroxide, NH2-SAPO-34-Pd/Co NPs were used as labels and immobilized secondary antibody (Ab2) through adsorption capacity of Pd/Co NPs to protein. The immunosensor displayed a wide linear range (0.001-20 ng/mL) and low detection limit (0.33 pg/mL). Good reproducibility and stability have showed satisfying results in the analysis of clinical urine samples. This novel and ultrasensitive immunosensor may have the potential application in the detection of different tumor markers.

No MeSH data available.


Related in: MedlinePlus