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Sensitive Electrochemical Immunosensor for Detection of Nuclear Matrix Protein-22 based on NH2-SAPO-34 Supported Pd/Co Nanoparticles.

Wu D, Wang Y, Zhang Y, Ma H, Yan T, Du B, Wei Q - Sci Rep (2016)

Bottom Line: The reduced graphene oxide-NH (rGO-NH) with good conductivity and large surface area was used to immobilize primary antibody (Ab1).Good reproducibility and stability have showed satisfying results in the analysis of clinical urine samples.This novel and ultrasensitive immunosensor may have the potential application in the detection of different tumor markers.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.

ABSTRACT
A novel sandwich-type electrochemical immunosensor using the new amino group functionalized silicoaluminophosphates molecular sieves (NH2-SAPO-34) supported Pd/Co nanoparticles (NH2-SAPO-34-Pd/Co NPs) as labels for the detection of bladder cancer biomarker nuclear matrix protein-22 (NMP-22) was developed in this work. The reduced graphene oxide-NH (rGO-NH) with good conductivity and large surface area was used to immobilize primary antibody (Ab1). Due to the excellent catalytic activity toward hydrogen peroxide, NH2-SAPO-34-Pd/Co NPs were used as labels and immobilized secondary antibody (Ab2) through adsorption capacity of Pd/Co NPs to protein. The immunosensor displayed a wide linear range (0.001-20 ng/mL) and low detection limit (0.33 pg/mL). Good reproducibility and stability have showed satisfying results in the analysis of clinical urine samples. This novel and ultrasensitive immunosensor may have the potential application in the detection of different tumor markers.

No MeSH data available.


Related in: MedlinePlus

SEM images of rGO-NH (a,b) NH2-SAPO-34 (c) and NH2-SAPO-34-Pd/Co NPs (d,e); EDS of NH2-SAPO-34-Pd/Co NPs (f).
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f2: SEM images of rGO-NH (a,b) NH2-SAPO-34 (c) and NH2-SAPO-34-Pd/Co NPs (d,e); EDS of NH2-SAPO-34-Pd/Co NPs (f).

Mentions: The rGO-NH with large surface area was used to increase the amount of captured Ab1. The SEM images (Fig. 2a,b) of the rGO-NH showed that the rGO-NH had a wrinkle paper-like structure with irregular size, further indicating the large surface area of rGO-NH. The SEM image of NH2-SAPO-34 was shown in Fig. 2c, which showed the NH2-SAPO-34 possessed cubic structure. The NH2-SAPO-34 had a BET surface area of 536.6 m2/g. Due to the large surface area, more Pd/Co nanoparticles could be loaded on the surface of NH2-SAPO-34 and the SEM images (Fig. 2d,e) of NH2-SAPO-34-Pd/Co presented that the NH2-SAPO-34 was coated successfully. Elemental compositions of NH2-SAPO-34-Pd/Co NPs were analyzed by EDS (Fig. 2f). Signature peaks of Si, O, P, Al, Pd and Co were observed, indicating that the Pd/Co nanoparticles were formed successfully on the surface of NH2-SAPO-34.


Sensitive Electrochemical Immunosensor for Detection of Nuclear Matrix Protein-22 based on NH2-SAPO-34 Supported Pd/Co Nanoparticles.

Wu D, Wang Y, Zhang Y, Ma H, Yan T, Du B, Wei Q - Sci Rep (2016)

SEM images of rGO-NH (a,b) NH2-SAPO-34 (c) and NH2-SAPO-34-Pd/Co NPs (d,e); EDS of NH2-SAPO-34-Pd/Co NPs (f).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834490&req=5

f2: SEM images of rGO-NH (a,b) NH2-SAPO-34 (c) and NH2-SAPO-34-Pd/Co NPs (d,e); EDS of NH2-SAPO-34-Pd/Co NPs (f).
Mentions: The rGO-NH with large surface area was used to increase the amount of captured Ab1. The SEM images (Fig. 2a,b) of the rGO-NH showed that the rGO-NH had a wrinkle paper-like structure with irregular size, further indicating the large surface area of rGO-NH. The SEM image of NH2-SAPO-34 was shown in Fig. 2c, which showed the NH2-SAPO-34 possessed cubic structure. The NH2-SAPO-34 had a BET surface area of 536.6 m2/g. Due to the large surface area, more Pd/Co nanoparticles could be loaded on the surface of NH2-SAPO-34 and the SEM images (Fig. 2d,e) of NH2-SAPO-34-Pd/Co presented that the NH2-SAPO-34 was coated successfully. Elemental compositions of NH2-SAPO-34-Pd/Co NPs were analyzed by EDS (Fig. 2f). Signature peaks of Si, O, P, Al, Pd and Co were observed, indicating that the Pd/Co nanoparticles were formed successfully on the surface of NH2-SAPO-34.

Bottom Line: The reduced graphene oxide-NH (rGO-NH) with good conductivity and large surface area was used to immobilize primary antibody (Ab1).Good reproducibility and stability have showed satisfying results in the analysis of clinical urine samples.This novel and ultrasensitive immunosensor may have the potential application in the detection of different tumor markers.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Chemical Sensing &Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P.R. China.

ABSTRACT
A novel sandwich-type electrochemical immunosensor using the new amino group functionalized silicoaluminophosphates molecular sieves (NH2-SAPO-34) supported Pd/Co nanoparticles (NH2-SAPO-34-Pd/Co NPs) as labels for the detection of bladder cancer biomarker nuclear matrix protein-22 (NMP-22) was developed in this work. The reduced graphene oxide-NH (rGO-NH) with good conductivity and large surface area was used to immobilize primary antibody (Ab1). Due to the excellent catalytic activity toward hydrogen peroxide, NH2-SAPO-34-Pd/Co NPs were used as labels and immobilized secondary antibody (Ab2) through adsorption capacity of Pd/Co NPs to protein. The immunosensor displayed a wide linear range (0.001-20 ng/mL) and low detection limit (0.33 pg/mL). Good reproducibility and stability have showed satisfying results in the analysis of clinical urine samples. This novel and ultrasensitive immunosensor may have the potential application in the detection of different tumor markers.

No MeSH data available.


Related in: MedlinePlus