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Hemoglobin consumption by P. falciparum in individual erythrocytes imaged via quantitative phase spectroscopy.

Rinehart MT, Park HS, Walzer KA, Chi JT, Wax A - Sci Rep (2016)

Bottom Line: QPS captures hyperspectral holograms of individual RBCs to measure spectroscopic changes across the visible wavelength range (475-700 nm), providing complex information, i.e. amplitude and phase, about the light field which has interacted with the cell.Hb content progressively decreases with parasite life cycle, with an average 72.2% reduction observed for RBCs infected by schizont-stage P. falciparum compared to uninfected cells.The unique ability of QPS to discriminate individual healthy and infected cells using spectroscopic changes indicates that the approach can be used to detect disease.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Biomedical Engineering, Duke University, Durham, NC 27708, US.

ABSTRACT
Plasmodium falciparum infection causes structural and biochemical changes in red blood cells (RBCs). To quantify these changes, we apply a novel optical technique, quantitative phase spectroscopy (QPS) to characterize individual red blood cells (RBCs) during the intraerythrocytic life cycle of P. falciparum. QPS captures hyperspectral holograms of individual RBCs to measure spectroscopic changes across the visible wavelength range (475-700 nm), providing complex information, i.e. amplitude and phase, about the light field which has interacted with the cell. The complex field provides complimentary information on hemoglobin content and cell mass, which are both found to dramatically change upon infection by P. falciparum. Hb content progressively decreases with parasite life cycle, with an average 72.2% reduction observed for RBCs infected by schizont-stage P. falciparum compared to uninfected cells. Infection also resulted in a 33.1% reduction in RBC's optical volume, a measure of the cells' non-aqueous components. Notably, optical volume is only partially correlated with hemoglobin content, suggesting that changes in other dry mass components such as parasite mass may also be assessed using this technique. The unique ability of QPS to discriminate individual healthy and infected cells using spectroscopic changes indicates that the approach can be used to detect disease.

No MeSH data available.


Related in: MedlinePlus

Relationship between non-spectroscopic OV and mass of hemoglobin within individual cells.Both infected and uninfected cells show only a weak trend between these metrics.
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f6: Relationship between non-spectroscopic OV and mass of hemoglobin within individual cells.Both infected and uninfected cells show only a weak trend between these metrics.

Mentions: As a final analysis, the relationship between the total optical volume for a cell, i.e. the average OV across the visible spectrum, and the fitted mass of hemoglobin of each cell is examined (Fig. 6). It appears that for both uninfected and infected cells hemoglobin content is only a modest predictor of cell optical volume (coefficient of determination r2 = 0.675). Thus, combining both hemoglobin mass and averaged OV could improve the ability to discriminate between these cell types as compared to using a single metric alone.


Hemoglobin consumption by P. falciparum in individual erythrocytes imaged via quantitative phase spectroscopy.

Rinehart MT, Park HS, Walzer KA, Chi JT, Wax A - Sci Rep (2016)

Relationship between non-spectroscopic OV and mass of hemoglobin within individual cells.Both infected and uninfected cells show only a weak trend between these metrics.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834482&req=5

f6: Relationship between non-spectroscopic OV and mass of hemoglobin within individual cells.Both infected and uninfected cells show only a weak trend between these metrics.
Mentions: As a final analysis, the relationship between the total optical volume for a cell, i.e. the average OV across the visible spectrum, and the fitted mass of hemoglobin of each cell is examined (Fig. 6). It appears that for both uninfected and infected cells hemoglobin content is only a modest predictor of cell optical volume (coefficient of determination r2 = 0.675). Thus, combining both hemoglobin mass and averaged OV could improve the ability to discriminate between these cell types as compared to using a single metric alone.

Bottom Line: QPS captures hyperspectral holograms of individual RBCs to measure spectroscopic changes across the visible wavelength range (475-700 nm), providing complex information, i.e. amplitude and phase, about the light field which has interacted with the cell.Hb content progressively decreases with parasite life cycle, with an average 72.2% reduction observed for RBCs infected by schizont-stage P. falciparum compared to uninfected cells.The unique ability of QPS to discriminate individual healthy and infected cells using spectroscopic changes indicates that the approach can be used to detect disease.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Biomedical Engineering, Duke University, Durham, NC 27708, US.

ABSTRACT
Plasmodium falciparum infection causes structural and biochemical changes in red blood cells (RBCs). To quantify these changes, we apply a novel optical technique, quantitative phase spectroscopy (QPS) to characterize individual red blood cells (RBCs) during the intraerythrocytic life cycle of P. falciparum. QPS captures hyperspectral holograms of individual RBCs to measure spectroscopic changes across the visible wavelength range (475-700 nm), providing complex information, i.e. amplitude and phase, about the light field which has interacted with the cell. The complex field provides complimentary information on hemoglobin content and cell mass, which are both found to dramatically change upon infection by P. falciparum. Hb content progressively decreases with parasite life cycle, with an average 72.2% reduction observed for RBCs infected by schizont-stage P. falciparum compared to uninfected cells. Infection also resulted in a 33.1% reduction in RBC's optical volume, a measure of the cells' non-aqueous components. Notably, optical volume is only partially correlated with hemoglobin content, suggesting that changes in other dry mass components such as parasite mass may also be assessed using this technique. The unique ability of QPS to discriminate individual healthy and infected cells using spectroscopic changes indicates that the approach can be used to detect disease.

No MeSH data available.


Related in: MedlinePlus