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Hemoglobin consumption by P. falciparum in individual erythrocytes imaged via quantitative phase spectroscopy.

Rinehart MT, Park HS, Walzer KA, Chi JT, Wax A - Sci Rep (2016)

Bottom Line: QPS captures hyperspectral holograms of individual RBCs to measure spectroscopic changes across the visible wavelength range (475-700 nm), providing complex information, i.e. amplitude and phase, about the light field which has interacted with the cell.Hb content progressively decreases with parasite life cycle, with an average 72.2% reduction observed for RBCs infected by schizont-stage P. falciparum compared to uninfected cells.The unique ability of QPS to discriminate individual healthy and infected cells using spectroscopic changes indicates that the approach can be used to detect disease.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Biomedical Engineering, Duke University, Durham, NC 27708, US.

ABSTRACT
Plasmodium falciparum infection causes structural and biochemical changes in red blood cells (RBCs). To quantify these changes, we apply a novel optical technique, quantitative phase spectroscopy (QPS) to characterize individual red blood cells (RBCs) during the intraerythrocytic life cycle of P. falciparum. QPS captures hyperspectral holograms of individual RBCs to measure spectroscopic changes across the visible wavelength range (475-700 nm), providing complex information, i.e. amplitude and phase, about the light field which has interacted with the cell. The complex field provides complimentary information on hemoglobin content and cell mass, which are both found to dramatically change upon infection by P. falciparum. Hb content progressively decreases with parasite life cycle, with an average 72.2% reduction observed for RBCs infected by schizont-stage P. falciparum compared to uninfected cells. Infection also resulted in a 33.1% reduction in RBC's optical volume, a measure of the cells' non-aqueous components. Notably, optical volume is only partially correlated with hemoglobin content, suggesting that changes in other dry mass components such as parasite mass may also be assessed using this technique. The unique ability of QPS to discriminate individual healthy and infected cells using spectroscopic changes indicates that the approach can be used to detect disease.

No MeSH data available.


Related in: MedlinePlus

(A) Molar extinction coefficients of oxy-hemoglobin28 and P. falciparum by-product hemozoin2729. (B) Corresponding relative RI increments calculated via the Kramers-Kronig relations19.
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f3: (A) Molar extinction coefficients of oxy-hemoglobin28 and P. falciparum by-product hemozoin2729. (B) Corresponding relative RI increments calculated via the Kramers-Kronig relations19.

Mentions: P. falciparum grows within RBCs and consumes hemoglobin as a fuel source. The consumption of hemoglobin produces free heme that would otherwise be toxic both to the parasite and the RBC. To mitigate this toxicity, P. falciparum creates insoluble heme dimer crystals called hemozoin. In addition to the morphological changes of infected RBCs, this process reduces the total amount of hemoglobin within a cell and creates the hemozoin byproduct with its own distinct spectral features27. Figure 3 presents the molar extinction coefficients of oxy-hemoglobin28 and hemozoin2729 and the respective corresponding relative RI increments, as calculated by the subtractive Kramers-Kronig relations referenced to λ0 = 800 nm19.


Hemoglobin consumption by P. falciparum in individual erythrocytes imaged via quantitative phase spectroscopy.

Rinehart MT, Park HS, Walzer KA, Chi JT, Wax A - Sci Rep (2016)

(A) Molar extinction coefficients of oxy-hemoglobin28 and P. falciparum by-product hemozoin2729. (B) Corresponding relative RI increments calculated via the Kramers-Kronig relations19.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834482&req=5

f3: (A) Molar extinction coefficients of oxy-hemoglobin28 and P. falciparum by-product hemozoin2729. (B) Corresponding relative RI increments calculated via the Kramers-Kronig relations19.
Mentions: P. falciparum grows within RBCs and consumes hemoglobin as a fuel source. The consumption of hemoglobin produces free heme that would otherwise be toxic both to the parasite and the RBC. To mitigate this toxicity, P. falciparum creates insoluble heme dimer crystals called hemozoin. In addition to the morphological changes of infected RBCs, this process reduces the total amount of hemoglobin within a cell and creates the hemozoin byproduct with its own distinct spectral features27. Figure 3 presents the molar extinction coefficients of oxy-hemoglobin28 and hemozoin2729 and the respective corresponding relative RI increments, as calculated by the subtractive Kramers-Kronig relations referenced to λ0 = 800 nm19.

Bottom Line: QPS captures hyperspectral holograms of individual RBCs to measure spectroscopic changes across the visible wavelength range (475-700 nm), providing complex information, i.e. amplitude and phase, about the light field which has interacted with the cell.Hb content progressively decreases with parasite life cycle, with an average 72.2% reduction observed for RBCs infected by schizont-stage P. falciparum compared to uninfected cells.The unique ability of QPS to discriminate individual healthy and infected cells using spectroscopic changes indicates that the approach can be used to detect disease.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Biomedical Engineering, Duke University, Durham, NC 27708, US.

ABSTRACT
Plasmodium falciparum infection causes structural and biochemical changes in red blood cells (RBCs). To quantify these changes, we apply a novel optical technique, quantitative phase spectroscopy (QPS) to characterize individual red blood cells (RBCs) during the intraerythrocytic life cycle of P. falciparum. QPS captures hyperspectral holograms of individual RBCs to measure spectroscopic changes across the visible wavelength range (475-700 nm), providing complex information, i.e. amplitude and phase, about the light field which has interacted with the cell. The complex field provides complimentary information on hemoglobin content and cell mass, which are both found to dramatically change upon infection by P. falciparum. Hb content progressively decreases with parasite life cycle, with an average 72.2% reduction observed for RBCs infected by schizont-stage P. falciparum compared to uninfected cells. Infection also resulted in a 33.1% reduction in RBC's optical volume, a measure of the cells' non-aqueous components. Notably, optical volume is only partially correlated with hemoglobin content, suggesting that changes in other dry mass components such as parasite mass may also be assessed using this technique. The unique ability of QPS to discriminate individual healthy and infected cells using spectroscopic changes indicates that the approach can be used to detect disease.

No MeSH data available.


Related in: MedlinePlus