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Stellate Cell Networks in the Teleost Pituitary.

Golan M, Hollander-Cohen L, Levavi-Sivan B - Sci Rep (2016)

Bottom Line: In the pars intermedia, stellate cells were arranged around neuronal bundles and their processes extended into the pars distalis.Within the pars distalis, stellate cells formed close associations with FSH cells and, to a lesser degree, with GH and LH cells, suggesting differential paracrine regulation of the two gonadotrope populations.The production of follistatin by stellate cells further corroborates the notion of a paracrine role on FSH release.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.

ABSTRACT
The folliculostellate cells of the mammalian pituitary are non-endocrine cells that are implicated in long-distance communication and paracrine signaling, but to date, these cells have yet to be characterized in teleosts. We found that the stellate cells of the teleost pituitary share many common attributes with mammalian folliculostellate cells. By labeling of stellate cells in live preparations of tilapia pituitaries we investigated their distribution, association with other endocrine cells and their anatomical and functional coupling. In the pars intermedia, stellate cells were arranged around neuronal bundles and their processes extended into the pars distalis. Within the pars distalis, stellate cells formed close associations with FSH cells and, to a lesser degree, with GH and LH cells, suggesting differential paracrine regulation of the two gonadotrope populations. The production of follistatin by stellate cells further corroborates the notion of a paracrine role on FSH release. We also found stellate cells to form gap junctions that enabled dye transfer to neighboring stellate cells, implicating that these cells form a large-scale network that connects distant parts of the pituitary. Our findings represent the first wide-scale study of stellate cells in teleosts and provide valuable information regarding their functional roles in pituitary function.

No MeSH data available.


Related in: MedlinePlus

Stellate cells produce follistatin.Imunnostaining of tilapia pituitaries for follistatin reveals that stellate cells (blue) contain follistatin (green). Staining is also apparent in non-stellate cells. Bar −5 μm.
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f4: Stellate cells produce follistatin.Imunnostaining of tilapia pituitaries for follistatin reveals that stellate cells (blue) contain follistatin (green). Staining is also apparent in non-stellate cells. Bar −5 μm.

Mentions: Further evidence for the expression of follistatin by stellate cells was provided by immunofluorescence. Staining tilapia pituitaries with an anti-follistatin antibody clearly labeled stellate cells (Fig. 4). Other cell types in the pituitary were also labeled, most notably the prolactin cells of the RPD (Supplementary Fig. 3). Pre-incubation of the antibody with follistatin drastically decreased staining intensity, demonstrating its specificity (Supplementary Fig. 3).


Stellate Cell Networks in the Teleost Pituitary.

Golan M, Hollander-Cohen L, Levavi-Sivan B - Sci Rep (2016)

Stellate cells produce follistatin.Imunnostaining of tilapia pituitaries for follistatin reveals that stellate cells (blue) contain follistatin (green). Staining is also apparent in non-stellate cells. Bar −5 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834476&req=5

f4: Stellate cells produce follistatin.Imunnostaining of tilapia pituitaries for follistatin reveals that stellate cells (blue) contain follistatin (green). Staining is also apparent in non-stellate cells. Bar −5 μm.
Mentions: Further evidence for the expression of follistatin by stellate cells was provided by immunofluorescence. Staining tilapia pituitaries with an anti-follistatin antibody clearly labeled stellate cells (Fig. 4). Other cell types in the pituitary were also labeled, most notably the prolactin cells of the RPD (Supplementary Fig. 3). Pre-incubation of the antibody with follistatin drastically decreased staining intensity, demonstrating its specificity (Supplementary Fig. 3).

Bottom Line: In the pars intermedia, stellate cells were arranged around neuronal bundles and their processes extended into the pars distalis.Within the pars distalis, stellate cells formed close associations with FSH cells and, to a lesser degree, with GH and LH cells, suggesting differential paracrine regulation of the two gonadotrope populations.The production of follistatin by stellate cells further corroborates the notion of a paracrine role on FSH release.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.

ABSTRACT
The folliculostellate cells of the mammalian pituitary are non-endocrine cells that are implicated in long-distance communication and paracrine signaling, but to date, these cells have yet to be characterized in teleosts. We found that the stellate cells of the teleost pituitary share many common attributes with mammalian folliculostellate cells. By labeling of stellate cells in live preparations of tilapia pituitaries we investigated their distribution, association with other endocrine cells and their anatomical and functional coupling. In the pars intermedia, stellate cells were arranged around neuronal bundles and their processes extended into the pars distalis. Within the pars distalis, stellate cells formed close associations with FSH cells and, to a lesser degree, with GH and LH cells, suggesting differential paracrine regulation of the two gonadotrope populations. The production of follistatin by stellate cells further corroborates the notion of a paracrine role on FSH release. We also found stellate cells to form gap junctions that enabled dye transfer to neighboring stellate cells, implicating that these cells form a large-scale network that connects distant parts of the pituitary. Our findings represent the first wide-scale study of stellate cells in teleosts and provide valuable information regarding their functional roles in pituitary function.

No MeSH data available.


Related in: MedlinePlus