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Stellate Cell Networks in the Teleost Pituitary.

Golan M, Hollander-Cohen L, Levavi-Sivan B - Sci Rep (2016)

Bottom Line: In the pars intermedia, stellate cells were arranged around neuronal bundles and their processes extended into the pars distalis.Within the pars distalis, stellate cells formed close associations with FSH cells and, to a lesser degree, with GH and LH cells, suggesting differential paracrine regulation of the two gonadotrope populations.The production of follistatin by stellate cells further corroborates the notion of a paracrine role on FSH release.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.

ABSTRACT
The folliculostellate cells of the mammalian pituitary are non-endocrine cells that are implicated in long-distance communication and paracrine signaling, but to date, these cells have yet to be characterized in teleosts. We found that the stellate cells of the teleost pituitary share many common attributes with mammalian folliculostellate cells. By labeling of stellate cells in live preparations of tilapia pituitaries we investigated their distribution, association with other endocrine cells and their anatomical and functional coupling. In the pars intermedia, stellate cells were arranged around neuronal bundles and their processes extended into the pars distalis. Within the pars distalis, stellate cells formed close associations with FSH cells and, to a lesser degree, with GH and LH cells, suggesting differential paracrine regulation of the two gonadotrope populations. The production of follistatin by stellate cells further corroborates the notion of a paracrine role on FSH release. We also found stellate cells to form gap junctions that enabled dye transfer to neighboring stellate cells, implicating that these cells form a large-scale network that connects distant parts of the pituitary. Our findings represent the first wide-scale study of stellate cells in teleosts and provide valuable information regarding their functional roles in pituitary function.

No MeSH data available.


Related in: MedlinePlus

Tilapia stellate cells express follistatin.AMCA-stained (a,c) and non-stained (b) pituitaries were FACS-analyzed to identify and isolate stellate cells. Stellate cells (blue) are small and non-granulated (evidenced by low FSC and SSC values, (c). Sorted cells were analyzed by real-time PCR (d,e). While the stellate cells fraction also contained impurities from other cell types (blue bars in (d), follistatin is more highly expressed in stellate cells than in the general population (e). (***p < 0.001, **p < 0.01).
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f3: Tilapia stellate cells express follistatin.AMCA-stained (a,c) and non-stained (b) pituitaries were FACS-analyzed to identify and isolate stellate cells. Stellate cells (blue) are small and non-granulated (evidenced by low FSC and SSC values, (c). Sorted cells were analyzed by real-time PCR (d,e). While the stellate cells fraction also contained impurities from other cell types (blue bars in (d), follistatin is more highly expressed in stellate cells than in the general population (e). (***p < 0.001, **p < 0.01).

Mentions: In order to further investigate the functional role of stellate cells we sorted cells from AMCA-stained pituitaries by FACS and analyzed their gene expression profiles by real-time PCR. Sorting parameters were optimized in comparison to a non-stained sample (Fig. 3a,b) and were set to exclude non-viable cells or cells with compromised membrane integrity (propidium iodide-stained cells). As expected, stellate cells comprised a relatively small (low forward-scatter (FSC) values) and non-granulated (low side-scatter (SSC) values) population when compared to the other cell types of the pituitary (Fig. 3c). Real-time PCR analysis of stained and non-stained cells revealed that the sorting procedure did not result in a completely pure stellate cells population since the stained sample also included cells that express pituitary hormones, albeit on a considerably lower level than in the general cell population (Fig. 3d). Of the genes tested, the only gene that exhibited higher expression levels in the stained population was follistatin (Fig. 3e), a known marker of folliculostellate cells22232425 and an inhibitor of FSH synthesis and release262728293031.


Stellate Cell Networks in the Teleost Pituitary.

Golan M, Hollander-Cohen L, Levavi-Sivan B - Sci Rep (2016)

Tilapia stellate cells express follistatin.AMCA-stained (a,c) and non-stained (b) pituitaries were FACS-analyzed to identify and isolate stellate cells. Stellate cells (blue) are small and non-granulated (evidenced by low FSC and SSC values, (c). Sorted cells were analyzed by real-time PCR (d,e). While the stellate cells fraction also contained impurities from other cell types (blue bars in (d), follistatin is more highly expressed in stellate cells than in the general population (e). (***p < 0.001, **p < 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834476&req=5

f3: Tilapia stellate cells express follistatin.AMCA-stained (a,c) and non-stained (b) pituitaries were FACS-analyzed to identify and isolate stellate cells. Stellate cells (blue) are small and non-granulated (evidenced by low FSC and SSC values, (c). Sorted cells were analyzed by real-time PCR (d,e). While the stellate cells fraction also contained impurities from other cell types (blue bars in (d), follistatin is more highly expressed in stellate cells than in the general population (e). (***p < 0.001, **p < 0.01).
Mentions: In order to further investigate the functional role of stellate cells we sorted cells from AMCA-stained pituitaries by FACS and analyzed their gene expression profiles by real-time PCR. Sorting parameters were optimized in comparison to a non-stained sample (Fig. 3a,b) and were set to exclude non-viable cells or cells with compromised membrane integrity (propidium iodide-stained cells). As expected, stellate cells comprised a relatively small (low forward-scatter (FSC) values) and non-granulated (low side-scatter (SSC) values) population when compared to the other cell types of the pituitary (Fig. 3c). Real-time PCR analysis of stained and non-stained cells revealed that the sorting procedure did not result in a completely pure stellate cells population since the stained sample also included cells that express pituitary hormones, albeit on a considerably lower level than in the general cell population (Fig. 3d). Of the genes tested, the only gene that exhibited higher expression levels in the stained population was follistatin (Fig. 3e), a known marker of folliculostellate cells22232425 and an inhibitor of FSH synthesis and release262728293031.

Bottom Line: In the pars intermedia, stellate cells were arranged around neuronal bundles and their processes extended into the pars distalis.Within the pars distalis, stellate cells formed close associations with FSH cells and, to a lesser degree, with GH and LH cells, suggesting differential paracrine regulation of the two gonadotrope populations.The production of follistatin by stellate cells further corroborates the notion of a paracrine role on FSH release.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.

ABSTRACT
The folliculostellate cells of the mammalian pituitary are non-endocrine cells that are implicated in long-distance communication and paracrine signaling, but to date, these cells have yet to be characterized in teleosts. We found that the stellate cells of the teleost pituitary share many common attributes with mammalian folliculostellate cells. By labeling of stellate cells in live preparations of tilapia pituitaries we investigated their distribution, association with other endocrine cells and their anatomical and functional coupling. In the pars intermedia, stellate cells were arranged around neuronal bundles and their processes extended into the pars distalis. Within the pars distalis, stellate cells formed close associations with FSH cells and, to a lesser degree, with GH and LH cells, suggesting differential paracrine regulation of the two gonadotrope populations. The production of follistatin by stellate cells further corroborates the notion of a paracrine role on FSH release. We also found stellate cells to form gap junctions that enabled dye transfer to neighboring stellate cells, implicating that these cells form a large-scale network that connects distant parts of the pituitary. Our findings represent the first wide-scale study of stellate cells in teleosts and provide valuable information regarding their functional roles in pituitary function.

No MeSH data available.


Related in: MedlinePlus