Limits...
Stellate Cell Networks in the Teleost Pituitary.

Golan M, Hollander-Cohen L, Levavi-Sivan B - Sci Rep (2016)

Bottom Line: In the pars intermedia, stellate cells were arranged around neuronal bundles and their processes extended into the pars distalis.Within the pars distalis, stellate cells formed close associations with FSH cells and, to a lesser degree, with GH and LH cells, suggesting differential paracrine regulation of the two gonadotrope populations.The production of follistatin by stellate cells further corroborates the notion of a paracrine role on FSH release.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.

ABSTRACT
The folliculostellate cells of the mammalian pituitary are non-endocrine cells that are implicated in long-distance communication and paracrine signaling, but to date, these cells have yet to be characterized in teleosts. We found that the stellate cells of the teleost pituitary share many common attributes with mammalian folliculostellate cells. By labeling of stellate cells in live preparations of tilapia pituitaries we investigated their distribution, association with other endocrine cells and their anatomical and functional coupling. In the pars intermedia, stellate cells were arranged around neuronal bundles and their processes extended into the pars distalis. Within the pars distalis, stellate cells formed close associations with FSH cells and, to a lesser degree, with GH and LH cells, suggesting differential paracrine regulation of the two gonadotrope populations. The production of follistatin by stellate cells further corroborates the notion of a paracrine role on FSH release. We also found stellate cells to form gap junctions that enabled dye transfer to neighboring stellate cells, implicating that these cells form a large-scale network that connects distant parts of the pituitary. Our findings represent the first wide-scale study of stellate cells in teleosts and provide valuable information regarding their functional roles in pituitary function.

No MeSH data available.


Related in: MedlinePlus

Labeling of stellate cells in the tilapia pituitary.(a) Sagittal section through a labeled pituitary (adult female, anterior left) shows stained cells (blue) in the dorsal PN, PI and PPD. No staining is observed in the ventral PN and in the RPD. Bar −100 μm. (b) Counter-staining of nuclei (red, propidium iodide) shows the angular and elongated nuclei of stellate cells (blue). (c) Surface rendering reveals long cytoplasmic processes that interconnect neighboring stellate cells to create a continuous structural network (width:246.0317 microns, height: 246.0317 microns, depth: 9.5673 microns. 19 slices. Bar −10 μm).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4834476&req=5

f1: Labeling of stellate cells in the tilapia pituitary.(a) Sagittal section through a labeled pituitary (adult female, anterior left) shows stained cells (blue) in the dorsal PN, PI and PPD. No staining is observed in the ventral PN and in the RPD. Bar −100 μm. (b) Counter-staining of nuclei (red, propidium iodide) shows the angular and elongated nuclei of stellate cells (blue). (c) Surface rendering reveals long cytoplasmic processes that interconnect neighboring stellate cells to create a continuous structural network (width:246.0317 microns, height: 246.0317 microns, depth: 9.5673 microns. 19 slices. Bar −10 μm).

Mentions: In order to label fish stellate cells we took advantage of their ability to perform specific uptake of fluorescent dipeptides20. In this manner, for the first time in fish, we were able to label these cells and study their unique distribution and architecture. Incubation with the dipeptide β-Ala-Lys-Nε-AMCA labeled a specific population of cells in the tilapia pituitary. Since the stained cells were not observed to form follicles at the ultrastructural level, we were not able to confidently use the term folliculostellate cells when referring to these cells. We thus chose to use the name stellate cells to indicate their stellate shape. Stellate cells were distributed within the PI as well as in the PPD but were totally absent from the RPD and ventral PN (Fig. 1a). Stained cells were relatively small, polygonal and contained an ovoid nucleus occupying most of the cell’s volume (Fig. 1b). Their long cytoplasmic processes connected them to other stellate cells, which together form a continuous structural network (Fig. 1c).


Stellate Cell Networks in the Teleost Pituitary.

Golan M, Hollander-Cohen L, Levavi-Sivan B - Sci Rep (2016)

Labeling of stellate cells in the tilapia pituitary.(a) Sagittal section through a labeled pituitary (adult female, anterior left) shows stained cells (blue) in the dorsal PN, PI and PPD. No staining is observed in the ventral PN and in the RPD. Bar −100 μm. (b) Counter-staining of nuclei (red, propidium iodide) shows the angular and elongated nuclei of stellate cells (blue). (c) Surface rendering reveals long cytoplasmic processes that interconnect neighboring stellate cells to create a continuous structural network (width:246.0317 microns, height: 246.0317 microns, depth: 9.5673 microns. 19 slices. Bar −10 μm).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834476&req=5

f1: Labeling of stellate cells in the tilapia pituitary.(a) Sagittal section through a labeled pituitary (adult female, anterior left) shows stained cells (blue) in the dorsal PN, PI and PPD. No staining is observed in the ventral PN and in the RPD. Bar −100 μm. (b) Counter-staining of nuclei (red, propidium iodide) shows the angular and elongated nuclei of stellate cells (blue). (c) Surface rendering reveals long cytoplasmic processes that interconnect neighboring stellate cells to create a continuous structural network (width:246.0317 microns, height: 246.0317 microns, depth: 9.5673 microns. 19 slices. Bar −10 μm).
Mentions: In order to label fish stellate cells we took advantage of their ability to perform specific uptake of fluorescent dipeptides20. In this manner, for the first time in fish, we were able to label these cells and study their unique distribution and architecture. Incubation with the dipeptide β-Ala-Lys-Nε-AMCA labeled a specific population of cells in the tilapia pituitary. Since the stained cells were not observed to form follicles at the ultrastructural level, we were not able to confidently use the term folliculostellate cells when referring to these cells. We thus chose to use the name stellate cells to indicate their stellate shape. Stellate cells were distributed within the PI as well as in the PPD but were totally absent from the RPD and ventral PN (Fig. 1a). Stained cells were relatively small, polygonal and contained an ovoid nucleus occupying most of the cell’s volume (Fig. 1b). Their long cytoplasmic processes connected them to other stellate cells, which together form a continuous structural network (Fig. 1c).

Bottom Line: In the pars intermedia, stellate cells were arranged around neuronal bundles and their processes extended into the pars distalis.Within the pars distalis, stellate cells formed close associations with FSH cells and, to a lesser degree, with GH and LH cells, suggesting differential paracrine regulation of the two gonadotrope populations.The production of follistatin by stellate cells further corroborates the notion of a paracrine role on FSH release.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.

ABSTRACT
The folliculostellate cells of the mammalian pituitary are non-endocrine cells that are implicated in long-distance communication and paracrine signaling, but to date, these cells have yet to be characterized in teleosts. We found that the stellate cells of the teleost pituitary share many common attributes with mammalian folliculostellate cells. By labeling of stellate cells in live preparations of tilapia pituitaries we investigated their distribution, association with other endocrine cells and their anatomical and functional coupling. In the pars intermedia, stellate cells were arranged around neuronal bundles and their processes extended into the pars distalis. Within the pars distalis, stellate cells formed close associations with FSH cells and, to a lesser degree, with GH and LH cells, suggesting differential paracrine regulation of the two gonadotrope populations. The production of follistatin by stellate cells further corroborates the notion of a paracrine role on FSH release. We also found stellate cells to form gap junctions that enabled dye transfer to neighboring stellate cells, implicating that these cells form a large-scale network that connects distant parts of the pituitary. Our findings represent the first wide-scale study of stellate cells in teleosts and provide valuable information regarding their functional roles in pituitary function.

No MeSH data available.


Related in: MedlinePlus