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Carbonate Mineral Formation under the Influence of Limestone-Colonizing Actinobacteria: Morphology and Polymorphism.

Cao C, Jiang J, Sun H, Huang Y, Tao F, Lian B - Front Microbiol (2016)

Bottom Line: Mineralogical analyses showed that hexagonal prism calcite was only observed in the sub-surfaces of the mycelium pellets, which is a novel morphology mediated by microbes.Our analyses suggested that the effects of mycelium pellets as a molecular template almost gained an advantage over SMP both in crystal nucleation and growth, having nothing to do with biological activity.It is thereby convinced that lithophilous actinobacteria, S. luteogriseus DHS C014, owing to its advantageous genetic metabolism and filamentous structure, showed good biomineralization abilities, maybe it would have geoactive potential for biogenic carbonate in local microenvironments.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of SciencesGuiyang, China; Institute of Geochemistry, University of Chinese Academy of SciencesBeijing, China; The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal UniversityXuzhou, China.

ABSTRACT
Microorganisms and their biomineralization processes are widespread in almost every environment on earth. In this work, Streptomyces luteogriseus DHS C014, a dominant lithophilous actinobacteria isolated from microbial mats on limestone rocks, was used to investigate its potential biomineralization to allow a better understanding of bacterial contributions to carbonate mineralization in nature. The ammonium carbonate free-drift method was used with mycelium pellets, culture supernatant, and spent culture of the strain. Mineralogical analyses showed that hexagonal prism calcite was only observed in the sub-surfaces of the mycelium pellets, which is a novel morphology mediated by microbes. Hemispheroidal vaterite appeared in the presence of spent culture, mainly because of the effects of soluble microbial products (SMP) during mineralization. When using the culture supernatant, doughnut-like vaterite was favored by actinobacterial mycelia, which has not yet been captured in previous studies. Our analyses suggested that the effects of mycelium pellets as a molecular template almost gained an advantage over SMP both in crystal nucleation and growth, having nothing to do with biological activity. It is thereby convinced that lithophilous actinobacteria, S. luteogriseus DHS C014, owing to its advantageous genetic metabolism and filamentous structure, showed good biomineralization abilities, maybe it would have geoactive potential for biogenic carbonate in local microenvironments.

No MeSH data available.


Related in: MedlinePlus

Neighbor-joining tree based on 16S rRNA gene sequences. It shows the relationships between strain DHS C014 and partial species of the genus Streptomyces. The sequence of Kitasatospora arboriphila HKI0189T (AY442267) was used as out-group. Numbers at branch nodes are bootstrap values (1000 re-samplings). Bar, 0.005 sequence variation.
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Figure 4: Neighbor-joining tree based on 16S rRNA gene sequences. It shows the relationships between strain DHS C014 and partial species of the genus Streptomyces. The sequence of Kitasatospora arboriphila HKI0189T (AY442267) was used as out-group. Numbers at branch nodes are bootstrap values (1000 re-samplings). Bar, 0.005 sequence variation.

Mentions: After incubation on ISP 2 agar at 28°C for 21 days, aerial mycelia usually crimped into spiral spore chains, and some of them began to fragment into short rod-shape spores with smooth surfaces (Figure 3). The almost complete 16S rRNA gene (1475 bp) of the strain was sequenced and deposited in GenBank with accession number KP986577. The strain shared its highest levels of 16S rRNA gene sequence similarity with the closest type strain Streptomyces luteogriseus NBRC 13402T (99.9%), and for other species of the genus the similarities were below 99.5%. The phylogenetic tree, based on the neighbor-joining algorithm (Figure 4), showed that strain DHS C014 formed a distinct sub-branch with the closest types strain, S. luteogriseus NBRC 13402T, supported by a bootstrap value of 76%. Based on the morphological and genotypical properties, the strain was identified as S. luteogriseus DHS C014.


Carbonate Mineral Formation under the Influence of Limestone-Colonizing Actinobacteria: Morphology and Polymorphism.

Cao C, Jiang J, Sun H, Huang Y, Tao F, Lian B - Front Microbiol (2016)

Neighbor-joining tree based on 16S rRNA gene sequences. It shows the relationships between strain DHS C014 and partial species of the genus Streptomyces. The sequence of Kitasatospora arboriphila HKI0189T (AY442267) was used as out-group. Numbers at branch nodes are bootstrap values (1000 re-samplings). Bar, 0.005 sequence variation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834437&req=5

Figure 4: Neighbor-joining tree based on 16S rRNA gene sequences. It shows the relationships between strain DHS C014 and partial species of the genus Streptomyces. The sequence of Kitasatospora arboriphila HKI0189T (AY442267) was used as out-group. Numbers at branch nodes are bootstrap values (1000 re-samplings). Bar, 0.005 sequence variation.
Mentions: After incubation on ISP 2 agar at 28°C for 21 days, aerial mycelia usually crimped into spiral spore chains, and some of them began to fragment into short rod-shape spores with smooth surfaces (Figure 3). The almost complete 16S rRNA gene (1475 bp) of the strain was sequenced and deposited in GenBank with accession number KP986577. The strain shared its highest levels of 16S rRNA gene sequence similarity with the closest type strain Streptomyces luteogriseus NBRC 13402T (99.9%), and for other species of the genus the similarities were below 99.5%. The phylogenetic tree, based on the neighbor-joining algorithm (Figure 4), showed that strain DHS C014 formed a distinct sub-branch with the closest types strain, S. luteogriseus NBRC 13402T, supported by a bootstrap value of 76%. Based on the morphological and genotypical properties, the strain was identified as S. luteogriseus DHS C014.

Bottom Line: Mineralogical analyses showed that hexagonal prism calcite was only observed in the sub-surfaces of the mycelium pellets, which is a novel morphology mediated by microbes.Our analyses suggested that the effects of mycelium pellets as a molecular template almost gained an advantage over SMP both in crystal nucleation and growth, having nothing to do with biological activity.It is thereby convinced that lithophilous actinobacteria, S. luteogriseus DHS C014, owing to its advantageous genetic metabolism and filamentous structure, showed good biomineralization abilities, maybe it would have geoactive potential for biogenic carbonate in local microenvironments.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of SciencesGuiyang, China; Institute of Geochemistry, University of Chinese Academy of SciencesBeijing, China; The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal UniversityXuzhou, China.

ABSTRACT
Microorganisms and their biomineralization processes are widespread in almost every environment on earth. In this work, Streptomyces luteogriseus DHS C014, a dominant lithophilous actinobacteria isolated from microbial mats on limestone rocks, was used to investigate its potential biomineralization to allow a better understanding of bacterial contributions to carbonate mineralization in nature. The ammonium carbonate free-drift method was used with mycelium pellets, culture supernatant, and spent culture of the strain. Mineralogical analyses showed that hexagonal prism calcite was only observed in the sub-surfaces of the mycelium pellets, which is a novel morphology mediated by microbes. Hemispheroidal vaterite appeared in the presence of spent culture, mainly because of the effects of soluble microbial products (SMP) during mineralization. When using the culture supernatant, doughnut-like vaterite was favored by actinobacterial mycelia, which has not yet been captured in previous studies. Our analyses suggested that the effects of mycelium pellets as a molecular template almost gained an advantage over SMP both in crystal nucleation and growth, having nothing to do with biological activity. It is thereby convinced that lithophilous actinobacteria, S. luteogriseus DHS C014, owing to its advantageous genetic metabolism and filamentous structure, showed good biomineralization abilities, maybe it would have geoactive potential for biogenic carbonate in local microenvironments.

No MeSH data available.


Related in: MedlinePlus