Limits...
Carbonate Mineral Formation under the Influence of Limestone-Colonizing Actinobacteria: Morphology and Polymorphism.

Cao C, Jiang J, Sun H, Huang Y, Tao F, Lian B - Front Microbiol (2016)

Bottom Line: Mineralogical analyses showed that hexagonal prism calcite was only observed in the sub-surfaces of the mycelium pellets, which is a novel morphology mediated by microbes.Our analyses suggested that the effects of mycelium pellets as a molecular template almost gained an advantage over SMP both in crystal nucleation and growth, having nothing to do with biological activity.It is thereby convinced that lithophilous actinobacteria, S. luteogriseus DHS C014, owing to its advantageous genetic metabolism and filamentous structure, showed good biomineralization abilities, maybe it would have geoactive potential for biogenic carbonate in local microenvironments.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of SciencesGuiyang, China; Institute of Geochemistry, University of Chinese Academy of SciencesBeijing, China; The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal UniversityXuzhou, China.

ABSTRACT
Microorganisms and their biomineralization processes are widespread in almost every environment on earth. In this work, Streptomyces luteogriseus DHS C014, a dominant lithophilous actinobacteria isolated from microbial mats on limestone rocks, was used to investigate its potential biomineralization to allow a better understanding of bacterial contributions to carbonate mineralization in nature. The ammonium carbonate free-drift method was used with mycelium pellets, culture supernatant, and spent culture of the strain. Mineralogical analyses showed that hexagonal prism calcite was only observed in the sub-surfaces of the mycelium pellets, which is a novel morphology mediated by microbes. Hemispheroidal vaterite appeared in the presence of spent culture, mainly because of the effects of soluble microbial products (SMP) during mineralization. When using the culture supernatant, doughnut-like vaterite was favored by actinobacterial mycelia, which has not yet been captured in previous studies. Our analyses suggested that the effects of mycelium pellets as a molecular template almost gained an advantage over SMP both in crystal nucleation and growth, having nothing to do with biological activity. It is thereby convinced that lithophilous actinobacteria, S. luteogriseus DHS C014, owing to its advantageous genetic metabolism and filamentous structure, showed good biomineralization abilities, maybe it would have geoactive potential for biogenic carbonate in local microenvironments.

No MeSH data available.


Related in: MedlinePlus

Map of Puding Karst Ecosystem Research Station (PKERS) and geochemical analyses of the mineral samples by XRD and SEM. Panel (A) Showing the sampling site; (B) showing X-ray powder diffraction patterns of the mineral samples [Numbers in the parentheses indicate the Miller indices, whereas C and D denote calcite and dolomite, respectively]; (C) showing the microbial mats on limestone rocks; (D–F) showing filamentous microorganisms on limestone rocks.
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Figure 1: Map of Puding Karst Ecosystem Research Station (PKERS) and geochemical analyses of the mineral samples by XRD and SEM. Panel (A) Showing the sampling site; (B) showing X-ray powder diffraction patterns of the mineral samples [Numbers in the parentheses indicate the Miller indices, whereas C and D denote calcite and dolomite, respectively]; (C) showing the microbial mats on limestone rocks; (D–F) showing filamentous microorganisms on limestone rocks.

Mentions: Limestone samples used for microbial isolation were collected at the Puding Karst Ecosystem Research Station (PKERS) of the Chinese Academy of Sciences in Guizhou Province, China (26°09′–26°31′N, 105°27′–105°58′E; Figure 1A). X-ray powder diffraction data (XRD Bruker D8-ADVANCE) showed that calcite was the dominant mineral phase of these limestone samples (Figure 1B). As shown in Figure 1C, limestone rocks were almost completely covered with microbial mats. In detail, many filamentous microorganisms living on the limestone were observed using scanning electron microscopy (SEM, Hitachi S-3400N; Figures 1D–F). X-ray fluorescence spectroscopy (XRF Bruker S8-TIGER equipped with 4 kW, Rh anode X-ray tube) showed that CaO, MgO, SiO2, Fe2O3, Al2O3, and CO2 accounted for 51.40, 3.99, 1.02, 0.23, 0.22, and 42.03% of the limestone by mass, respectively (data were expressed as oxides).


Carbonate Mineral Formation under the Influence of Limestone-Colonizing Actinobacteria: Morphology and Polymorphism.

Cao C, Jiang J, Sun H, Huang Y, Tao F, Lian B - Front Microbiol (2016)

Map of Puding Karst Ecosystem Research Station (PKERS) and geochemical analyses of the mineral samples by XRD and SEM. Panel (A) Showing the sampling site; (B) showing X-ray powder diffraction patterns of the mineral samples [Numbers in the parentheses indicate the Miller indices, whereas C and D denote calcite and dolomite, respectively]; (C) showing the microbial mats on limestone rocks; (D–F) showing filamentous microorganisms on limestone rocks.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834437&req=5

Figure 1: Map of Puding Karst Ecosystem Research Station (PKERS) and geochemical analyses of the mineral samples by XRD and SEM. Panel (A) Showing the sampling site; (B) showing X-ray powder diffraction patterns of the mineral samples [Numbers in the parentheses indicate the Miller indices, whereas C and D denote calcite and dolomite, respectively]; (C) showing the microbial mats on limestone rocks; (D–F) showing filamentous microorganisms on limestone rocks.
Mentions: Limestone samples used for microbial isolation were collected at the Puding Karst Ecosystem Research Station (PKERS) of the Chinese Academy of Sciences in Guizhou Province, China (26°09′–26°31′N, 105°27′–105°58′E; Figure 1A). X-ray powder diffraction data (XRD Bruker D8-ADVANCE) showed that calcite was the dominant mineral phase of these limestone samples (Figure 1B). As shown in Figure 1C, limestone rocks were almost completely covered with microbial mats. In detail, many filamentous microorganisms living on the limestone were observed using scanning electron microscopy (SEM, Hitachi S-3400N; Figures 1D–F). X-ray fluorescence spectroscopy (XRF Bruker S8-TIGER equipped with 4 kW, Rh anode X-ray tube) showed that CaO, MgO, SiO2, Fe2O3, Al2O3, and CO2 accounted for 51.40, 3.99, 1.02, 0.23, 0.22, and 42.03% of the limestone by mass, respectively (data were expressed as oxides).

Bottom Line: Mineralogical analyses showed that hexagonal prism calcite was only observed in the sub-surfaces of the mycelium pellets, which is a novel morphology mediated by microbes.Our analyses suggested that the effects of mycelium pellets as a molecular template almost gained an advantage over SMP both in crystal nucleation and growth, having nothing to do with biological activity.It is thereby convinced that lithophilous actinobacteria, S. luteogriseus DHS C014, owing to its advantageous genetic metabolism and filamentous structure, showed good biomineralization abilities, maybe it would have geoactive potential for biogenic carbonate in local microenvironments.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of SciencesGuiyang, China; Institute of Geochemistry, University of Chinese Academy of SciencesBeijing, China; The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal UniversityXuzhou, China.

ABSTRACT
Microorganisms and their biomineralization processes are widespread in almost every environment on earth. In this work, Streptomyces luteogriseus DHS C014, a dominant lithophilous actinobacteria isolated from microbial mats on limestone rocks, was used to investigate its potential biomineralization to allow a better understanding of bacterial contributions to carbonate mineralization in nature. The ammonium carbonate free-drift method was used with mycelium pellets, culture supernatant, and spent culture of the strain. Mineralogical analyses showed that hexagonal prism calcite was only observed in the sub-surfaces of the mycelium pellets, which is a novel morphology mediated by microbes. Hemispheroidal vaterite appeared in the presence of spent culture, mainly because of the effects of soluble microbial products (SMP) during mineralization. When using the culture supernatant, doughnut-like vaterite was favored by actinobacterial mycelia, which has not yet been captured in previous studies. Our analyses suggested that the effects of mycelium pellets as a molecular template almost gained an advantage over SMP both in crystal nucleation and growth, having nothing to do with biological activity. It is thereby convinced that lithophilous actinobacteria, S. luteogriseus DHS C014, owing to its advantageous genetic metabolism and filamentous structure, showed good biomineralization abilities, maybe it would have geoactive potential for biogenic carbonate in local microenvironments.

No MeSH data available.


Related in: MedlinePlus