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First TBEV serological screening in Flemish wild boar.

Roelandt S, Suin V, Van der Stede Y, Lamoral S, Marche S, Tignon M, Saiz JC, Escribano-Romero E, Casaer J, Brochier B, Van Gucht S, Roels S, Vervaeke M - Infect Ecol Epidemiol (2016)

Bottom Line: Seven wild boars were found TBEV-seropositive and showed moderate (>1/15) to high (>1/125) SNT-titres; three individuals had borderline results (1/10-1/15).This study demonstrated the presence of TBEV-specific antibodies in wild boar and highlighted potential TBEV-foci in Flanders.Additional surveillance including direct virus testing is now recommended.

View Article: PubMed Central - PubMed

Affiliation: Unit of Epidemiology, Risk Assessment and Surveillance (ERASURV), Operational Directorate of Interactions and Surveillance, Veterinary and Agrochemical Research Centre (CODA-CERVA), Brussels, Belgium; sophie.roelandt@coda-cerva.be.

ABSTRACT
In the frame of a Flemish wildlife surveillance in 2013, a serological screening was performed on sera from wild boar (Sus scrofa; n=238) in order to detect tick-borne encephalitis virus (TBEV)-specific antibodies. Neutralising antibodies were titrated with a seroneutralisation test (SNT), using two cut-off titres (1/10-1/15). Seven wild boars were found TBEV-seropositive and showed moderate (>1/15) to high (>1/125) SNT-titres; three individuals had borderline results (1/10-1/15). This study demonstrated the presence of TBEV-specific antibodies in wild boar and highlighted potential TBEV-foci in Flanders. Additional surveillance including direct virus testing is now recommended.

No MeSH data available.


Related in: MedlinePlus

Map of Wild boar sampling (left) and TBEV-seropositives (right) in Flanders. Left Part: Study population and positives per community; Right Part: Calculated wild boar TBEV-seroprevalence based on 10 SNT-reactors (positive/borderline – cut-off 1/10) out of 238 wild boar tested. FLA: Flanders total study population (n=238); WFL: West Flanders subpopulation (n=77); LIM: Limburg+Antwerp subpopulation (n=161).
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Figure 0001: Map of Wild boar sampling (left) and TBEV-seropositives (right) in Flanders. Left Part: Study population and positives per community; Right Part: Calculated wild boar TBEV-seroprevalence based on 10 SNT-reactors (positive/borderline – cut-off 1/10) out of 238 wild boar tested. FLA: Flanders total study population (n=238); WFL: West Flanders subpopulation (n=77); LIM: Limburg+Antwerp subpopulation (n=161).

Mentions: We used Flemish wild boar sera collected in 2013, within the frame of disease surveillance that was focused on Aujeszky's disease, Classical swine fever, and Brucellosis (7). The total target population size was estimated at roughly 3,000 heads (M. Vervaeke – ANB, pers. comm.). A total of 238 representative sera were obtained by veterinarians from the two Flemish wild boar subpopulations in different provinces: Limburg+Antwerp (LIM: n=161) and West Flanders (WFL: n=77). The study population and its seroprevalence (Fig. 1) were mapped using QGIS®2.2-Valmiera (www.qgis.org/), using a vector layer of Flanders in the Belgian Lambert 1972 EPSG-projection. Sample size calculations for disease detection and probability of freedom were performed in Survey Toolbox®1.04 (www.epitools.ausvet.com.au) and in WinEpiscope®2.0 (www.wageningenur.nl/).


First TBEV serological screening in Flemish wild boar.

Roelandt S, Suin V, Van der Stede Y, Lamoral S, Marche S, Tignon M, Saiz JC, Escribano-Romero E, Casaer J, Brochier B, Van Gucht S, Roels S, Vervaeke M - Infect Ecol Epidemiol (2016)

Map of Wild boar sampling (left) and TBEV-seropositives (right) in Flanders. Left Part: Study population and positives per community; Right Part: Calculated wild boar TBEV-seroprevalence based on 10 SNT-reactors (positive/borderline – cut-off 1/10) out of 238 wild boar tested. FLA: Flanders total study population (n=238); WFL: West Flanders subpopulation (n=77); LIM: Limburg+Antwerp subpopulation (n=161).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834417&req=5

Figure 0001: Map of Wild boar sampling (left) and TBEV-seropositives (right) in Flanders. Left Part: Study population and positives per community; Right Part: Calculated wild boar TBEV-seroprevalence based on 10 SNT-reactors (positive/borderline – cut-off 1/10) out of 238 wild boar tested. FLA: Flanders total study population (n=238); WFL: West Flanders subpopulation (n=77); LIM: Limburg+Antwerp subpopulation (n=161).
Mentions: We used Flemish wild boar sera collected in 2013, within the frame of disease surveillance that was focused on Aujeszky's disease, Classical swine fever, and Brucellosis (7). The total target population size was estimated at roughly 3,000 heads (M. Vervaeke – ANB, pers. comm.). A total of 238 representative sera were obtained by veterinarians from the two Flemish wild boar subpopulations in different provinces: Limburg+Antwerp (LIM: n=161) and West Flanders (WFL: n=77). The study population and its seroprevalence (Fig. 1) were mapped using QGIS®2.2-Valmiera (www.qgis.org/), using a vector layer of Flanders in the Belgian Lambert 1972 EPSG-projection. Sample size calculations for disease detection and probability of freedom were performed in Survey Toolbox®1.04 (www.epitools.ausvet.com.au) and in WinEpiscope®2.0 (www.wageningenur.nl/).

Bottom Line: Seven wild boars were found TBEV-seropositive and showed moderate (>1/15) to high (>1/125) SNT-titres; three individuals had borderline results (1/10-1/15).This study demonstrated the presence of TBEV-specific antibodies in wild boar and highlighted potential TBEV-foci in Flanders.Additional surveillance including direct virus testing is now recommended.

View Article: PubMed Central - PubMed

Affiliation: Unit of Epidemiology, Risk Assessment and Surveillance (ERASURV), Operational Directorate of Interactions and Surveillance, Veterinary and Agrochemical Research Centre (CODA-CERVA), Brussels, Belgium; sophie.roelandt@coda-cerva.be.

ABSTRACT
In the frame of a Flemish wildlife surveillance in 2013, a serological screening was performed on sera from wild boar (Sus scrofa; n=238) in order to detect tick-borne encephalitis virus (TBEV)-specific antibodies. Neutralising antibodies were titrated with a seroneutralisation test (SNT), using two cut-off titres (1/10-1/15). Seven wild boars were found TBEV-seropositive and showed moderate (>1/15) to high (>1/125) SNT-titres; three individuals had borderline results (1/10-1/15). This study demonstrated the presence of TBEV-specific antibodies in wild boar and highlighted potential TBEV-foci in Flanders. Additional surveillance including direct virus testing is now recommended.

No MeSH data available.


Related in: MedlinePlus