Limits...
Expression of MicroRNA-155 is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B Patients.

Yu SL, Deng H, Li XH, Huang YX, Xie DY, Gao ZL - Hepat Mon (2016)

Bottom Line: In the HBV-infected patients, the miR-155 levels were significantly lower than in the healthy controls (P = 0.001).Chronic HBV-infected patients with elevated ALT had higher levels of miR-155 compared with patients with normal ALT (P = 0.014).No correlations were found between miR-155 and ALT or HBV DNA.

View Article: PubMed Central - PubMed

Affiliation: Department of Infectious Diseases, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

ABSTRACT

Background: Persistent hepatitis B virus (HBV) infection is sustained by inadequate immune responses, either natural or acquired. Recent studies have suggested that immune responses to viral infection may be affected by microRNA (miR)-155, via its involvement in immune cell differentiation and maturation. However, little is known on the specific interaction between miR-155 and HBV in host antiviral immunity.

Objectives: This study evaluated the levels of miR-155 in peripheral blood mononuclear cells (PBMCs) of chronic hepatitis B (CHB) patients, relative to that of healthy subjects, and investigated an association between miR-155 levels and HBV DNA or alanine aminotransferase (ALT).

Patients and methods: Total RNA was extracted from peripheral venous blood samples of 90 treatment-naive patients with chronic HBV infection and 20 healthy volunteers. The levels of miR-155 in the PBMCs were measured by real-time quantitative polymerase chain reaction. Serum HBV DNA and liver enzymes were estimated using standard clinical laboratory methods.

Results: In the HBV-infected patients, the miR-155 levels were significantly lower than in the healthy controls (P = 0.001). Chronic HBV-infected patients with elevated ALT had higher levels of miR-155 compared with patients with normal ALT (P = 0.014). No correlations were found between miR-155 and ALT or HBV DNA.

Conclusions: The miR-155 appeared to be suppressed during HBV infection. The significantly higher miR-155 levels in ALT-elevated patients infected with HBV suggest that miR-155 levels in PBMCs correlate with the immune state of patients with chronic HBV infection.

No MeSH data available.


Related in: MedlinePlus

Normalized Levels of MiR-155 in Peripheral Blood Mononuclear Cells of Hepatitis B Virus-Infected Patients and Healthy ControlsA, levels of miR-155, represented as relative quantitation (RQ), were significantly lower in HBV-infected patients compared with healthy controls (P = 0.001); B, patients with elevated ALT had significantly higher levels of miR-155 than did patients with normal ALT (P = 0.014). Results are expressed as mean of RQ ± standard error of the mean; Abbreviations: ALT, alanine transaminase; CHB, chronic hepatitis B.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4834416&req=5

fig28704: Normalized Levels of MiR-155 in Peripheral Blood Mononuclear Cells of Hepatitis B Virus-Infected Patients and Healthy ControlsA, levels of miR-155, represented as relative quantitation (RQ), were significantly lower in HBV-infected patients compared with healthy controls (P = 0.001); B, patients with elevated ALT had significantly higher levels of miR-155 than did patients with normal ALT (P = 0.014). Results are expressed as mean of RQ ± standard error of the mean; Abbreviations: ALT, alanine transaminase; CHB, chronic hepatitis B.

Mentions: The levels of miR-155 in the PBMC samples were quantified via real-time qPCR (Figure 1). MiRNA levels of U6 snRNA were used to normalize the Ct value. The analysis revealed that the miR-155 levels of the CHB patients were significantly lower than those of the healthy controls (P = 0.001; Figure 1A). This suggested that circulating miR-155 may negatively correlate with chronic HBV infection.


Expression of MicroRNA-155 is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B Patients.

Yu SL, Deng H, Li XH, Huang YX, Xie DY, Gao ZL - Hepat Mon (2016)

Normalized Levels of MiR-155 in Peripheral Blood Mononuclear Cells of Hepatitis B Virus-Infected Patients and Healthy ControlsA, levels of miR-155, represented as relative quantitation (RQ), were significantly lower in HBV-infected patients compared with healthy controls (P = 0.001); B, patients with elevated ALT had significantly higher levels of miR-155 than did patients with normal ALT (P = 0.014). Results are expressed as mean of RQ ± standard error of the mean; Abbreviations: ALT, alanine transaminase; CHB, chronic hepatitis B.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834416&req=5

fig28704: Normalized Levels of MiR-155 in Peripheral Blood Mononuclear Cells of Hepatitis B Virus-Infected Patients and Healthy ControlsA, levels of miR-155, represented as relative quantitation (RQ), were significantly lower in HBV-infected patients compared with healthy controls (P = 0.001); B, patients with elevated ALT had significantly higher levels of miR-155 than did patients with normal ALT (P = 0.014). Results are expressed as mean of RQ ± standard error of the mean; Abbreviations: ALT, alanine transaminase; CHB, chronic hepatitis B.
Mentions: The levels of miR-155 in the PBMC samples were quantified via real-time qPCR (Figure 1). MiRNA levels of U6 snRNA were used to normalize the Ct value. The analysis revealed that the miR-155 levels of the CHB patients were significantly lower than those of the healthy controls (P = 0.001; Figure 1A). This suggested that circulating miR-155 may negatively correlate with chronic HBV infection.

Bottom Line: In the HBV-infected patients, the miR-155 levels were significantly lower than in the healthy controls (P = 0.001).Chronic HBV-infected patients with elevated ALT had higher levels of miR-155 compared with patients with normal ALT (P = 0.014).No correlations were found between miR-155 and ALT or HBV DNA.

View Article: PubMed Central - PubMed

Affiliation: Department of Infectious Diseases, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

ABSTRACT

Background: Persistent hepatitis B virus (HBV) infection is sustained by inadequate immune responses, either natural or acquired. Recent studies have suggested that immune responses to viral infection may be affected by microRNA (miR)-155, via its involvement in immune cell differentiation and maturation. However, little is known on the specific interaction between miR-155 and HBV in host antiviral immunity.

Objectives: This study evaluated the levels of miR-155 in peripheral blood mononuclear cells (PBMCs) of chronic hepatitis B (CHB) patients, relative to that of healthy subjects, and investigated an association between miR-155 levels and HBV DNA or alanine aminotransferase (ALT).

Patients and methods: Total RNA was extracted from peripheral venous blood samples of 90 treatment-naive patients with chronic HBV infection and 20 healthy volunteers. The levels of miR-155 in the PBMCs were measured by real-time quantitative polymerase chain reaction. Serum HBV DNA and liver enzymes were estimated using standard clinical laboratory methods.

Results: In the HBV-infected patients, the miR-155 levels were significantly lower than in the healthy controls (P = 0.001). Chronic HBV-infected patients with elevated ALT had higher levels of miR-155 compared with patients with normal ALT (P = 0.014). No correlations were found between miR-155 and ALT or HBV DNA.

Conclusions: The miR-155 appeared to be suppressed during HBV infection. The significantly higher miR-155 levels in ALT-elevated patients infected with HBV suggest that miR-155 levels in PBMCs correlate with the immune state of patients with chronic HBV infection.

No MeSH data available.


Related in: MedlinePlus