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XPC Promotes Pluripotency of Human Dental Pulp Cells through Regulation of Oct-4/Sox2/c-Myc.

Liu L, Peng Z, Xu Z, Wei X - Stem Cells Int (2016)

Bottom Line: XPC, Oct-4, Sox2, and c-Myc were downregulated at P7 compared with P3 in DPCs with long-term culture.Oct-4/Sox2/c-Myc were significantly upregulated, and multilineage differentiation in DPCs with XPC overexpression was enhanced after transfection.Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Operative Dentistry and Endodontics, Guanghua School of Stomatology, Affiliated Stomatological Hospital, Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong 510055, China.

ABSTRACT
Introduction. Xeroderma pigmentosum group C (XPC), essential component of multisubunit stem cell coactivator complex (SCC), functions as the critical factor modulating pluripotency and genome integrity through interaction with Oct-4/Sox2. However, its specific role in regulating pluripotency and multilineage differentiation of human dental pulp cells (DPCs) remains unknown. Methods. To elucidate the functional role XPC played in pluripotency and multilineage differentiation of DPCs, expressions of XPC in DPCs with long-term culture were examined by real-time PCR and western blot. DPCs were transfected with lentiviral-mediated human XPC gene; then transfection rate was investigated by real-time PCR and western blot. Cell cycle, apoptosis, proliferation, senescence, multilineage differentiation, and expression of Oct-4/Sox2/c-Myc in transfected DPCs were examined. Results. XPC, Oct-4, Sox2, and c-Myc were downregulated at P7 compared with P3 in DPCs with long-term culture. XPC genes were upregulated in DPCs at P2 after transfection and maintained high expression level at P3 and P7. Cell proliferation, PI value, and telomerase activity were enhanced, whereas apoptosis was suppressed in transfected DPCs. Oct-4/Sox2/c-Myc were significantly upregulated, and multilineage differentiation in DPCs with XPC overexpression was enhanced after transfection. Conclusions. XPC plays an essential role in the modulation of pluripotency and multilineage differentiation of DPCs through regulation of Oct-4/Sox2/c-Myc.

No MeSH data available.


Related in: MedlinePlus

Expression of XPC, Oct-4, Sox2, and c-Myc in DPCs at various passages. Real-time PCR showed that mRNA expression of XPC, Oct-4, Sox2, and c-Myc was significantly higher in DPCs at passage 3 compared with passage 7 (a) (∗p < 0.05, ∗∗p < 0.001). Western blot showed that the protein expression of XPC, Oct-4, Sox2, and c-Myc revealed similar expression pattern with real-time PCR, which was stronger in DPCs at passage 3 compared with passage 7 (b).
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fig1: Expression of XPC, Oct-4, Sox2, and c-Myc in DPCs at various passages. Real-time PCR showed that mRNA expression of XPC, Oct-4, Sox2, and c-Myc was significantly higher in DPCs at passage 3 compared with passage 7 (a) (∗p < 0.05, ∗∗p < 0.001). Western blot showed that the protein expression of XPC, Oct-4, Sox2, and c-Myc revealed similar expression pattern with real-time PCR, which was stronger in DPCs at passage 3 compared with passage 7 (b).

Mentions: Expression of XPC in DPCs from passages 1 to 7 were investigated by real-time PCR and western blot, while only the results of representative passages (P3 and P7) were presented. Real-time PCR (Figure 1(a)) and western blot (Figure 1(b)) indicated that the mRNA and protein expression of XPC, Oct-4, Sox2, and c-Myc showed similar expression pattern, which was significantly downregulated at P7 compared with P3 (∗p < 0.05, ∗∗p < 0.001).


XPC Promotes Pluripotency of Human Dental Pulp Cells through Regulation of Oct-4/Sox2/c-Myc.

Liu L, Peng Z, Xu Z, Wei X - Stem Cells Int (2016)

Expression of XPC, Oct-4, Sox2, and c-Myc in DPCs at various passages. Real-time PCR showed that mRNA expression of XPC, Oct-4, Sox2, and c-Myc was significantly higher in DPCs at passage 3 compared with passage 7 (a) (∗p < 0.05, ∗∗p < 0.001). Western blot showed that the protein expression of XPC, Oct-4, Sox2, and c-Myc revealed similar expression pattern with real-time PCR, which was stronger in DPCs at passage 3 compared with passage 7 (b).
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4834411&req=5

fig1: Expression of XPC, Oct-4, Sox2, and c-Myc in DPCs at various passages. Real-time PCR showed that mRNA expression of XPC, Oct-4, Sox2, and c-Myc was significantly higher in DPCs at passage 3 compared with passage 7 (a) (∗p < 0.05, ∗∗p < 0.001). Western blot showed that the protein expression of XPC, Oct-4, Sox2, and c-Myc revealed similar expression pattern with real-time PCR, which was stronger in DPCs at passage 3 compared with passage 7 (b).
Mentions: Expression of XPC in DPCs from passages 1 to 7 were investigated by real-time PCR and western blot, while only the results of representative passages (P3 and P7) were presented. Real-time PCR (Figure 1(a)) and western blot (Figure 1(b)) indicated that the mRNA and protein expression of XPC, Oct-4, Sox2, and c-Myc showed similar expression pattern, which was significantly downregulated at P7 compared with P3 (∗p < 0.05, ∗∗p < 0.001).

Bottom Line: XPC, Oct-4, Sox2, and c-Myc were downregulated at P7 compared with P3 in DPCs with long-term culture.Oct-4/Sox2/c-Myc were significantly upregulated, and multilineage differentiation in DPCs with XPC overexpression was enhanced after transfection.Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Operative Dentistry and Endodontics, Guanghua School of Stomatology, Affiliated Stomatological Hospital, Guangdong Province Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong 510055, China.

ABSTRACT
Introduction. Xeroderma pigmentosum group C (XPC), essential component of multisubunit stem cell coactivator complex (SCC), functions as the critical factor modulating pluripotency and genome integrity through interaction with Oct-4/Sox2. However, its specific role in regulating pluripotency and multilineage differentiation of human dental pulp cells (DPCs) remains unknown. Methods. To elucidate the functional role XPC played in pluripotency and multilineage differentiation of DPCs, expressions of XPC in DPCs with long-term culture were examined by real-time PCR and western blot. DPCs were transfected with lentiviral-mediated human XPC gene; then transfection rate was investigated by real-time PCR and western blot. Cell cycle, apoptosis, proliferation, senescence, multilineage differentiation, and expression of Oct-4/Sox2/c-Myc in transfected DPCs were examined. Results. XPC, Oct-4, Sox2, and c-Myc were downregulated at P7 compared with P3 in DPCs with long-term culture. XPC genes were upregulated in DPCs at P2 after transfection and maintained high expression level at P3 and P7. Cell proliferation, PI value, and telomerase activity were enhanced, whereas apoptosis was suppressed in transfected DPCs. Oct-4/Sox2/c-Myc were significantly upregulated, and multilineage differentiation in DPCs with XPC overexpression was enhanced after transfection. Conclusions. XPC plays an essential role in the modulation of pluripotency and multilineage differentiation of DPCs through regulation of Oct-4/Sox2/c-Myc.

No MeSH data available.


Related in: MedlinePlus