Limits...
The Antibacterial Activity of Chitosan Products Blended with Monoterpenes and Their Biofilms against Plant Pathogenic Bacteria.

Badawy ME, Rabea EI, Taktak NE, El-Nouby MA - Scientifica (Cairo) (2016)

Bottom Line: The antibacterial activity was evaluated in vitro by the agar dilution technique as a minimum inhibitory concentration (MIC) that was found to be dependent on the type of the microorganism tested.The most active product of chitosan was used for biofilm production enriched with geraniol and thymol (0.1 and 0.5%) and the films were also evaluated against the tested bacteria.The biological bioactivities summarized here may provide novel insights into the functions of chitosan and some monoterpenes and potentially allow their use for food protection from microbial attack.

View Article: PubMed Central - PubMed

Affiliation: Department of Pesticide Chemistry and Technology, Faculty of Agriculture, Alexandria University, El-Shatby, Alexandria 21545, Egypt.

ABSTRACT
This study focuses on the biological activities of eleven chitosan products with a viscosity-average molecular weight ranging from 22 to 846 kDa in combination with the most active monoterpenes (geraniol and thymol), out of 10 tested, against four plant pathogenic bacteria, Agrobacterium tumefaciens, Erwinia carotovora, Corynebacterium fascians, and Pseudomonas solanacearum. The antibacterial activity was evaluated in vitro by the agar dilution technique as a minimum inhibitory concentration (MIC) that was found to be dependent on the type of the microorganism tested. The most active product of chitosan was used for biofilm production enriched with geraniol and thymol (0.1 and 0.5%) and the films were also evaluated against the tested bacteria. The biological bioactivities summarized here may provide novel insights into the functions of chitosan and some monoterpenes and potentially allow their use for food protection from microbial attack.

No MeSH data available.


Related in: MedlinePlus

Photographs of chitosan composite films on the left (Ch/starch + 0.5% geraniol and Ch/starch + 0.5% thymol) and SEM micrographs of the surface of the films on the right. Scale bar 1 μm and magnification ×10000 for surface morphologies of the film.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4834403&req=5

fig1: Photographs of chitosan composite films on the left (Ch/starch + 0.5% geraniol and Ch/starch + 0.5% thymol) and SEM micrographs of the surface of the films on the right. Scale bar 1 μm and magnification ×10000 for surface morphologies of the film.

Mentions: In an attempt to study the microstructural changes in the films, SEM was conducted to visualize the surface of prepared films. Figure 1 shows photographs of chitosan composite films (Ch/starch + 0.5% geraniol and Ch/starch + 0.5% thymol) and SEM micrographs of the surface of the films. SEM microphotographs of the surfaces indicated a compact and homogenous structure without any large pores. A smooth, continuous structure was observed for the chitosan films surface; the presence of plasticizers caused continuities associated with the presence of geraniol or thymol in the matrix.


The Antibacterial Activity of Chitosan Products Blended with Monoterpenes and Their Biofilms against Plant Pathogenic Bacteria.

Badawy ME, Rabea EI, Taktak NE, El-Nouby MA - Scientifica (Cairo) (2016)

Photographs of chitosan composite films on the left (Ch/starch + 0.5% geraniol and Ch/starch + 0.5% thymol) and SEM micrographs of the surface of the films on the right. Scale bar 1 μm and magnification ×10000 for surface morphologies of the film.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4834403&req=5

fig1: Photographs of chitosan composite films on the left (Ch/starch + 0.5% geraniol and Ch/starch + 0.5% thymol) and SEM micrographs of the surface of the films on the right. Scale bar 1 μm and magnification ×10000 for surface morphologies of the film.
Mentions: In an attempt to study the microstructural changes in the films, SEM was conducted to visualize the surface of prepared films. Figure 1 shows photographs of chitosan composite films (Ch/starch + 0.5% geraniol and Ch/starch + 0.5% thymol) and SEM micrographs of the surface of the films. SEM microphotographs of the surfaces indicated a compact and homogenous structure without any large pores. A smooth, continuous structure was observed for the chitosan films surface; the presence of plasticizers caused continuities associated with the presence of geraniol or thymol in the matrix.

Bottom Line: The antibacterial activity was evaluated in vitro by the agar dilution technique as a minimum inhibitory concentration (MIC) that was found to be dependent on the type of the microorganism tested.The most active product of chitosan was used for biofilm production enriched with geraniol and thymol (0.1 and 0.5%) and the films were also evaluated against the tested bacteria.The biological bioactivities summarized here may provide novel insights into the functions of chitosan and some monoterpenes and potentially allow their use for food protection from microbial attack.

View Article: PubMed Central - PubMed

Affiliation: Department of Pesticide Chemistry and Technology, Faculty of Agriculture, Alexandria University, El-Shatby, Alexandria 21545, Egypt.

ABSTRACT
This study focuses on the biological activities of eleven chitosan products with a viscosity-average molecular weight ranging from 22 to 846 kDa in combination with the most active monoterpenes (geraniol and thymol), out of 10 tested, against four plant pathogenic bacteria, Agrobacterium tumefaciens, Erwinia carotovora, Corynebacterium fascians, and Pseudomonas solanacearum. The antibacterial activity was evaluated in vitro by the agar dilution technique as a minimum inhibitory concentration (MIC) that was found to be dependent on the type of the microorganism tested. The most active product of chitosan was used for biofilm production enriched with geraniol and thymol (0.1 and 0.5%) and the films were also evaluated against the tested bacteria. The biological bioactivities summarized here may provide novel insights into the functions of chitosan and some monoterpenes and potentially allow their use for food protection from microbial attack.

No MeSH data available.


Related in: MedlinePlus