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Colocalization of Mating-Induced Fos and D2-Like Dopamine Receptors in the Medial Preoptic Area: Influence of Sexual Experience.

Nutsch VL, Will RG, Robison CL, Martz JR, Tobiansky DJ, Dominguez JM - Front Behav Neurosci (2016)

Bottom Line: Sexually experienced animals also had significantly more D2-positive cells.Sexually inexperienced animals copulating for the first time had a larger percentage of D2-positive cells containing Fos, when compared to sexually experienced animals.Finally, regardless of experience, animals that had sex prior to sacrifice had significantly more D2-positive cells that contained Fos, vs. animals that did not copulate.

View Article: PubMed Central - PubMed

Affiliation: Institute for Neuroscience, The University of Texas at Austin Austin, TX, USA.

ABSTRACT
Dopamine in the medial preoptic area (mPOA) stimulates sexual activity in males. This is evidenced by microdialysis and microinjection experiments revealing that dopamine receptor antagonists in the mPOA inhibit sexual activity, whereas agonists facilitate behavior. Microdialysis experiments similarly show a facilitative role for dopamine, as levels of dopamine in the mPOA increase with mating. While the majority of evidence suggests an important role for dopamine receptors in the mPOA in the regulation of male sexual behaviors, whether sexual activity or sexual experience influence dopamine receptor function in the mPOA has not been previously shown. Here we used immunohistochemical assays to determine whether varying levels of sexual activity or experience influence the number of cells containing Fos or D2 receptor immunoreactivity. Results show that sexual experience facilitated subsequent behavior, namely experience decreased latencies. Moreover, the number of cells with immunoreactivity for Fos or D2 correlated with levels of sexual experience and sexual activity. Sexual activity increased Fos immunoreactivity. Sexually experienced animals also had significantly more D2-positive cells. Sexually inexperienced animals copulating for the first time had a larger percentage of D2-positive cells containing Fos, when compared to sexually experienced animals. Finally, regardless of experience, animals that had sex prior to sacrifice had significantly more D2-positive cells that contained Fos, vs. animals that did not copulate. These findings are noteworthy because sexually experienced animals display increased sexual efficiency. The differences in activation of D2 and changes in receptor density may play a role in this efficiency and other behavioral changes across sexual experience.

No MeSH data available.


Related in: MedlinePlus

Effects of sexual activity and sexual experience on D2- and Fos-immunoreactivity in the mPOA of male rats. (A) Animals who copulated prior to sacrifice had significantly more Fos-positive cells. (B) Animals with sexual experience had significantly more D2-immunopositive cells compared to naïve animals. (C) There was an interaction between sex and experience on the percentage of D2 cells that contained Fos; specifically, naïve animals copulating for the first time had a significantly higher percent of D2 cells that expressed Fos. (*p < 0.05).
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Figure 2: Effects of sexual activity and sexual experience on D2- and Fos-immunoreactivity in the mPOA of male rats. (A) Animals who copulated prior to sacrifice had significantly more Fos-positive cells. (B) Animals with sexual experience had significantly more D2-immunopositive cells compared to naïve animals. (C) There was an interaction between sex and experience on the percentage of D2 cells that contained Fos; specifically, naïve animals copulating for the first time had a significantly higher percent of D2 cells that expressed Fos. (*p < 0.05).

Mentions: A two way ANOVA revealed a significant main effect of sex on the number of Fos-positive cells in the mPOA (F(1,40) = 178.915, p < 0.001), whereby animals that had sex prior to sacrifice had significantly more Fos-ir cells. However, there was not a main effect of experience (F(1,40) = 0.800, p = 0.376), nor was there a sex by experience interaction (F(1,40) = 0.562, p = 0.4579; Figure 2A). A heat map depicting the relationship between the number of Fos-positive cells and sexual behavior is presented in Figure 3A.


Colocalization of Mating-Induced Fos and D2-Like Dopamine Receptors in the Medial Preoptic Area: Influence of Sexual Experience.

Nutsch VL, Will RG, Robison CL, Martz JR, Tobiansky DJ, Dominguez JM - Front Behav Neurosci (2016)

Effects of sexual activity and sexual experience on D2- and Fos-immunoreactivity in the mPOA of male rats. (A) Animals who copulated prior to sacrifice had significantly more Fos-positive cells. (B) Animals with sexual experience had significantly more D2-immunopositive cells compared to naïve animals. (C) There was an interaction between sex and experience on the percentage of D2 cells that contained Fos; specifically, naïve animals copulating for the first time had a significantly higher percent of D2 cells that expressed Fos. (*p < 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834303&req=5

Figure 2: Effects of sexual activity and sexual experience on D2- and Fos-immunoreactivity in the mPOA of male rats. (A) Animals who copulated prior to sacrifice had significantly more Fos-positive cells. (B) Animals with sexual experience had significantly more D2-immunopositive cells compared to naïve animals. (C) There was an interaction between sex and experience on the percentage of D2 cells that contained Fos; specifically, naïve animals copulating for the first time had a significantly higher percent of D2 cells that expressed Fos. (*p < 0.05).
Mentions: A two way ANOVA revealed a significant main effect of sex on the number of Fos-positive cells in the mPOA (F(1,40) = 178.915, p < 0.001), whereby animals that had sex prior to sacrifice had significantly more Fos-ir cells. However, there was not a main effect of experience (F(1,40) = 0.800, p = 0.376), nor was there a sex by experience interaction (F(1,40) = 0.562, p = 0.4579; Figure 2A). A heat map depicting the relationship between the number of Fos-positive cells and sexual behavior is presented in Figure 3A.

Bottom Line: Sexually experienced animals also had significantly more D2-positive cells.Sexually inexperienced animals copulating for the first time had a larger percentage of D2-positive cells containing Fos, when compared to sexually experienced animals.Finally, regardless of experience, animals that had sex prior to sacrifice had significantly more D2-positive cells that contained Fos, vs. animals that did not copulate.

View Article: PubMed Central - PubMed

Affiliation: Institute for Neuroscience, The University of Texas at Austin Austin, TX, USA.

ABSTRACT
Dopamine in the medial preoptic area (mPOA) stimulates sexual activity in males. This is evidenced by microdialysis and microinjection experiments revealing that dopamine receptor antagonists in the mPOA inhibit sexual activity, whereas agonists facilitate behavior. Microdialysis experiments similarly show a facilitative role for dopamine, as levels of dopamine in the mPOA increase with mating. While the majority of evidence suggests an important role for dopamine receptors in the mPOA in the regulation of male sexual behaviors, whether sexual activity or sexual experience influence dopamine receptor function in the mPOA has not been previously shown. Here we used immunohistochemical assays to determine whether varying levels of sexual activity or experience influence the number of cells containing Fos or D2 receptor immunoreactivity. Results show that sexual experience facilitated subsequent behavior, namely experience decreased latencies. Moreover, the number of cells with immunoreactivity for Fos or D2 correlated with levels of sexual experience and sexual activity. Sexual activity increased Fos immunoreactivity. Sexually experienced animals also had significantly more D2-positive cells. Sexually inexperienced animals copulating for the first time had a larger percentage of D2-positive cells containing Fos, when compared to sexually experienced animals. Finally, regardless of experience, animals that had sex prior to sacrifice had significantly more D2-positive cells that contained Fos, vs. animals that did not copulate. These findings are noteworthy because sexually experienced animals display increased sexual efficiency. The differences in activation of D2 and changes in receptor density may play a role in this efficiency and other behavioral changes across sexual experience.

No MeSH data available.


Related in: MedlinePlus