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A Carotenoid-Deficient Mutant in Pantoea sp. YR343, a Bacteria Isolated from the Rhizosphere of Populus deltoides, Is Defective in Root Colonization.

Bible AN, Fletcher SJ, Pelletier DA, Schadt CW, Jawdy SS, Weston DJ, Engle NL, Tschaplinski T, Masyuko R, Polisetti S, Bohn PW, Coutinho TA, Doktycz MJ, Morrell-Falvey JL - Front Microbiol (2016)

Bottom Line: The complex interactions between plants and their microbiome can have a profound effect on the health and productivity of the plant host.Finally we demonstrate that the ΔcrtB mutant shows reduced colonization of plant roots.YR343.

View Article: PubMed Central - PubMed

Affiliation: Biosciences Division, Oak Ridge National Laboratory Oak Ridge, TN, USA.

ABSTRACT
The complex interactions between plants and their microbiome can have a profound effect on the health and productivity of the plant host. A better understanding of the microbial mechanisms that promote plant health and stress tolerance will enable strategies for improving the productivity of economically important plants. Pantoea sp. YR343 is a motile, rod-shaped bacterium isolated from the roots of Populus deltoides that possesses the ability to solubilize phosphate and produce the phytohormone indole-3-acetic acid (IAA). Pantoea sp. YR343 readily colonizes plant roots and does not appear to be pathogenic when applied to the leaves or roots of selected plant hosts. To better understand the molecular mechanisms involved in plant association and rhizosphere survival by Pantoea sp. YR343, we constructed a mutant in which the crtB gene encoding phytoene synthase was deleted. Phytoene synthase is responsible for converting geranylgeranyl pyrophosphate to phytoene, an important precursor to the production of carotenoids. As predicted, the ΔcrtB mutant is defective in carotenoid production, and shows increased sensitivity to oxidative stress. Moreover, we find that the ΔcrtB mutant is impaired in biofilm formation and production of IAA. Finally we demonstrate that the ΔcrtB mutant shows reduced colonization of plant roots. Taken together, these data suggest that carotenoids are important for plant association and/or rhizosphere survival in Pantoea sp. YR343.

No MeSH data available.


Related in: MedlinePlus

Loss of carotenoids affects growth and phytohormone production. (A) Growth curves of wild type Pantoea sp. YR343 and ΔcrtB in M9 minimal medium. (B) Comparison of indole-3-acetic acid production in the wild type Pantoea sp. YR343 and the ΔcrtB mutant. The production of indoles was measured using a colorimetric assay.
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Figure 5: Loss of carotenoids affects growth and phytohormone production. (A) Growth curves of wild type Pantoea sp. YR343 and ΔcrtB in M9 minimal medium. (B) Comparison of indole-3-acetic acid production in the wild type Pantoea sp. YR343 and the ΔcrtB mutant. The production of indoles was measured using a colorimetric assay.

Mentions: Since carotenoids have also been implicated in modulating membrane properties (Huang and Haug, 1974; Chamberlain et al., 1991; Gruszecki, 1999; Gruszecki and Strzalka, 2005; Landrum, 2009; Lopez and Kolter, 2010), we next examined whether this carotenoid affected other biological behaviors, such as growth, IAA production, biofilm formation, and root colonization. Comparisons of growth in liquid cultures between wild type and the ΔcrtB mutant showed that the growth rates were very similar when cells were grown in LB or R2A medium (data not shown), but that the ΔcrtB mutant reached stationary phase at a lower cell density compared to wild type cells when grown in M9 minimal medium (Figure 5A).


A Carotenoid-Deficient Mutant in Pantoea sp. YR343, a Bacteria Isolated from the Rhizosphere of Populus deltoides, Is Defective in Root Colonization.

Bible AN, Fletcher SJ, Pelletier DA, Schadt CW, Jawdy SS, Weston DJ, Engle NL, Tschaplinski T, Masyuko R, Polisetti S, Bohn PW, Coutinho TA, Doktycz MJ, Morrell-Falvey JL - Front Microbiol (2016)

Loss of carotenoids affects growth and phytohormone production. (A) Growth curves of wild type Pantoea sp. YR343 and ΔcrtB in M9 minimal medium. (B) Comparison of indole-3-acetic acid production in the wild type Pantoea sp. YR343 and the ΔcrtB mutant. The production of indoles was measured using a colorimetric assay.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834302&req=5

Figure 5: Loss of carotenoids affects growth and phytohormone production. (A) Growth curves of wild type Pantoea sp. YR343 and ΔcrtB in M9 minimal medium. (B) Comparison of indole-3-acetic acid production in the wild type Pantoea sp. YR343 and the ΔcrtB mutant. The production of indoles was measured using a colorimetric assay.
Mentions: Since carotenoids have also been implicated in modulating membrane properties (Huang and Haug, 1974; Chamberlain et al., 1991; Gruszecki, 1999; Gruszecki and Strzalka, 2005; Landrum, 2009; Lopez and Kolter, 2010), we next examined whether this carotenoid affected other biological behaviors, such as growth, IAA production, biofilm formation, and root colonization. Comparisons of growth in liquid cultures between wild type and the ΔcrtB mutant showed that the growth rates were very similar when cells were grown in LB or R2A medium (data not shown), but that the ΔcrtB mutant reached stationary phase at a lower cell density compared to wild type cells when grown in M9 minimal medium (Figure 5A).

Bottom Line: The complex interactions between plants and their microbiome can have a profound effect on the health and productivity of the plant host.Finally we demonstrate that the ΔcrtB mutant shows reduced colonization of plant roots.YR343.

View Article: PubMed Central - PubMed

Affiliation: Biosciences Division, Oak Ridge National Laboratory Oak Ridge, TN, USA.

ABSTRACT
The complex interactions between plants and their microbiome can have a profound effect on the health and productivity of the plant host. A better understanding of the microbial mechanisms that promote plant health and stress tolerance will enable strategies for improving the productivity of economically important plants. Pantoea sp. YR343 is a motile, rod-shaped bacterium isolated from the roots of Populus deltoides that possesses the ability to solubilize phosphate and produce the phytohormone indole-3-acetic acid (IAA). Pantoea sp. YR343 readily colonizes plant roots and does not appear to be pathogenic when applied to the leaves or roots of selected plant hosts. To better understand the molecular mechanisms involved in plant association and rhizosphere survival by Pantoea sp. YR343, we constructed a mutant in which the crtB gene encoding phytoene synthase was deleted. Phytoene synthase is responsible for converting geranylgeranyl pyrophosphate to phytoene, an important precursor to the production of carotenoids. As predicted, the ΔcrtB mutant is defective in carotenoid production, and shows increased sensitivity to oxidative stress. Moreover, we find that the ΔcrtB mutant is impaired in biofilm formation and production of IAA. Finally we demonstrate that the ΔcrtB mutant shows reduced colonization of plant roots. Taken together, these data suggest that carotenoids are important for plant association and/or rhizosphere survival in Pantoea sp. YR343.

No MeSH data available.


Related in: MedlinePlus