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Application of ZnO Nanoparticles for Improving the Thermal and pH Stability of Crude Cellulase Obtained from Aspergillus fumigatus AA001.

Srivastava N, Srivastava M, Mishra PK, Ramteke PW - Front Microbiol (2016)

Bottom Line: Cellulases are the enzymes which are responsible for the hydrolysis of cellulosic biomass.The crude thermostable cellulase has been obtained from the Aspergillus fumigatus AA001 and treated with ZnO nanoparticle which shows thermal stability at 65°C up to 10 h whereas it showed pH stability in the alkaline pH range and retained its 53% of relative activity at pH 10.5.These findings may be promising in the area of biofuels production.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture Technology & SciencesAllahabad, India; Department of Chemical Engineering and Technology, Indian Institute of Technology (Banaras Hindu University)Varanasi, India.

ABSTRACT
Cellulases are the enzymes which are responsible for the hydrolysis of cellulosic biomass. In this study thermal and pH stability of crude cellulase has been investigated in the presence of zinc oxide (ZnO) nanoparticles. We synthesized ZnO nanoparticle by sol-gel method and characterized through various techniques including, X-ray Diffraction, ultraviolet-visible spectroscope, field emission scanning electron microscope and high resolution scanning electron microscope. The crude thermostable cellulase has been obtained from the Aspergillus fumigatus AA001 and treated with ZnO nanoparticle which shows thermal stability at 65°C up to 10 h whereas it showed pH stability in the alkaline pH range and retained its 53% of relative activity at pH 10.5. These findings may be promising in the area of biofuels production.

No MeSH data available.


(a) Field emission scanning electron microscope (FE-SEM) image (inset shows snap of real peanuts), (b) TEM (upper inset shows magnified image and lower inset shows SAED pattern), (c) HR-TEM micrograph and (d) histogram of ZnO nanopaticles fitted by Lorentzian function.
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Figure 2: (a) Field emission scanning electron microscope (FE-SEM) image (inset shows snap of real peanuts), (b) TEM (upper inset shows magnified image and lower inset shows SAED pattern), (c) HR-TEM micrograph and (d) histogram of ZnO nanopaticles fitted by Lorentzian function.

Mentions: Figure 2a represents FE-SEM micrograph of the ZnO nanoparticles. It can be seen that the surface morphology of ZnO resembles peanut types structure, and the particles are uniformly distributed over the entire micrograph. The grain size can be measured in the range of 0.1–1.0 μm. To observe the actual size and shape of the ZnO nanoparticles, HR-TEM micrographs are shown in Figures 2b,c. The HR-TEM micrographs show that particles are nearly spherical in shape and their size lies between 6 and 17 nm. The selected area electron diffraction pattern (SAED) (lower inset of Figure 2b) shows series of rings which define the polycrystalline nature of ZnO nanoparticles (Srivastava et al., 2013). Moreover, as presented in Figure 2c, the HR-TEM image suggest that the particles are well crystallized where the lattice fringes are clearly observed. The interplanar spacing of 0.280 nm, and 0.165 nm corresponding to (100) and (101) are in consistent with the XRD results. The quantitative measurement of the size of the particles is also done by plotting the histogram and fitted by Lorentzian function (Figure 2d). The average size of the particles from the histogram is measured to be 12.56 ± 0.26 nm (Srivastava et al., 2013).


Application of ZnO Nanoparticles for Improving the Thermal and pH Stability of Crude Cellulase Obtained from Aspergillus fumigatus AA001.

Srivastava N, Srivastava M, Mishra PK, Ramteke PW - Front Microbiol (2016)

(a) Field emission scanning electron microscope (FE-SEM) image (inset shows snap of real peanuts), (b) TEM (upper inset shows magnified image and lower inset shows SAED pattern), (c) HR-TEM micrograph and (d) histogram of ZnO nanopaticles fitted by Lorentzian function.
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Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4834293&req=5

Figure 2: (a) Field emission scanning electron microscope (FE-SEM) image (inset shows snap of real peanuts), (b) TEM (upper inset shows magnified image and lower inset shows SAED pattern), (c) HR-TEM micrograph and (d) histogram of ZnO nanopaticles fitted by Lorentzian function.
Mentions: Figure 2a represents FE-SEM micrograph of the ZnO nanoparticles. It can be seen that the surface morphology of ZnO resembles peanut types structure, and the particles are uniformly distributed over the entire micrograph. The grain size can be measured in the range of 0.1–1.0 μm. To observe the actual size and shape of the ZnO nanoparticles, HR-TEM micrographs are shown in Figures 2b,c. The HR-TEM micrographs show that particles are nearly spherical in shape and their size lies between 6 and 17 nm. The selected area electron diffraction pattern (SAED) (lower inset of Figure 2b) shows series of rings which define the polycrystalline nature of ZnO nanoparticles (Srivastava et al., 2013). Moreover, as presented in Figure 2c, the HR-TEM image suggest that the particles are well crystallized where the lattice fringes are clearly observed. The interplanar spacing of 0.280 nm, and 0.165 nm corresponding to (100) and (101) are in consistent with the XRD results. The quantitative measurement of the size of the particles is also done by plotting the histogram and fitted by Lorentzian function (Figure 2d). The average size of the particles from the histogram is measured to be 12.56 ± 0.26 nm (Srivastava et al., 2013).

Bottom Line: Cellulases are the enzymes which are responsible for the hydrolysis of cellulosic biomass.The crude thermostable cellulase has been obtained from the Aspergillus fumigatus AA001 and treated with ZnO nanoparticle which shows thermal stability at 65°C up to 10 h whereas it showed pH stability in the alkaline pH range and retained its 53% of relative activity at pH 10.5.These findings may be promising in the area of biofuels production.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture Technology & SciencesAllahabad, India; Department of Chemical Engineering and Technology, Indian Institute of Technology (Banaras Hindu University)Varanasi, India.

ABSTRACT
Cellulases are the enzymes which are responsible for the hydrolysis of cellulosic biomass. In this study thermal and pH stability of crude cellulase has been investigated in the presence of zinc oxide (ZnO) nanoparticles. We synthesized ZnO nanoparticle by sol-gel method and characterized through various techniques including, X-ray Diffraction, ultraviolet-visible spectroscope, field emission scanning electron microscope and high resolution scanning electron microscope. The crude thermostable cellulase has been obtained from the Aspergillus fumigatus AA001 and treated with ZnO nanoparticle which shows thermal stability at 65°C up to 10 h whereas it showed pH stability in the alkaline pH range and retained its 53% of relative activity at pH 10.5. These findings may be promising in the area of biofuels production.

No MeSH data available.