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Application of ZnO Nanoparticles for Improving the Thermal and pH Stability of Crude Cellulase Obtained from Aspergillus fumigatus AA001.

Srivastava N, Srivastava M, Mishra PK, Ramteke PW - Front Microbiol (2016)

Bottom Line: Cellulases are the enzymes which are responsible for the hydrolysis of cellulosic biomass.The crude thermostable cellulase has been obtained from the Aspergillus fumigatus AA001 and treated with ZnO nanoparticle which shows thermal stability at 65°C up to 10 h whereas it showed pH stability in the alkaline pH range and retained its 53% of relative activity at pH 10.5.These findings may be promising in the area of biofuels production.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture Technology & SciencesAllahabad, India; Department of Chemical Engineering and Technology, Indian Institute of Technology (Banaras Hindu University)Varanasi, India.

ABSTRACT
Cellulases are the enzymes which are responsible for the hydrolysis of cellulosic biomass. In this study thermal and pH stability of crude cellulase has been investigated in the presence of zinc oxide (ZnO) nanoparticles. We synthesized ZnO nanoparticle by sol-gel method and characterized through various techniques including, X-ray Diffraction, ultraviolet-visible spectroscope, field emission scanning electron microscope and high resolution scanning electron microscope. The crude thermostable cellulase has been obtained from the Aspergillus fumigatus AA001 and treated with ZnO nanoparticle which shows thermal stability at 65°C up to 10 h whereas it showed pH stability in the alkaline pH range and retained its 53% of relative activity at pH 10.5. These findings may be promising in the area of biofuels production.

No MeSH data available.


(A) X-ray Diffraction (XRD) pattern of the synthesized product, (B) UV-Vis spectrum of the ZnO nanoparticles and inset shows Tauc plot.
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Figure 1: (A) X-ray Diffraction (XRD) pattern of the synthesized product, (B) UV-Vis spectrum of the ZnO nanoparticles and inset shows Tauc plot.

Mentions: The XRD pattern of the synthesized product is shown in Figure 1A. The XRD pattern exhibits nine well defined diffraction peaks and all the peaks are indexed with the help of joint committee on powder diffraction standards (JCPDS card no. 36-1451) (Deng et al., 2016). These peaks are corresponding to (100), (002), (101), (102), (110), (103), (200), (112), and (201) planes which suggest the formation of wurtzite type crystal structure. Besides, no peak corresponding to any impurity could be observed and confirm the formation of single phase ZnO.


Application of ZnO Nanoparticles for Improving the Thermal and pH Stability of Crude Cellulase Obtained from Aspergillus fumigatus AA001.

Srivastava N, Srivastava M, Mishra PK, Ramteke PW - Front Microbiol (2016)

(A) X-ray Diffraction (XRD) pattern of the synthesized product, (B) UV-Vis spectrum of the ZnO nanoparticles and inset shows Tauc plot.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834293&req=5

Figure 1: (A) X-ray Diffraction (XRD) pattern of the synthesized product, (B) UV-Vis spectrum of the ZnO nanoparticles and inset shows Tauc plot.
Mentions: The XRD pattern of the synthesized product is shown in Figure 1A. The XRD pattern exhibits nine well defined diffraction peaks and all the peaks are indexed with the help of joint committee on powder diffraction standards (JCPDS card no. 36-1451) (Deng et al., 2016). These peaks are corresponding to (100), (002), (101), (102), (110), (103), (200), (112), and (201) planes which suggest the formation of wurtzite type crystal structure. Besides, no peak corresponding to any impurity could be observed and confirm the formation of single phase ZnO.

Bottom Line: Cellulases are the enzymes which are responsible for the hydrolysis of cellulosic biomass.The crude thermostable cellulase has been obtained from the Aspergillus fumigatus AA001 and treated with ZnO nanoparticle which shows thermal stability at 65°C up to 10 h whereas it showed pH stability in the alkaline pH range and retained its 53% of relative activity at pH 10.5.These findings may be promising in the area of biofuels production.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture Technology & SciencesAllahabad, India; Department of Chemical Engineering and Technology, Indian Institute of Technology (Banaras Hindu University)Varanasi, India.

ABSTRACT
Cellulases are the enzymes which are responsible for the hydrolysis of cellulosic biomass. In this study thermal and pH stability of crude cellulase has been investigated in the presence of zinc oxide (ZnO) nanoparticles. We synthesized ZnO nanoparticle by sol-gel method and characterized through various techniques including, X-ray Diffraction, ultraviolet-visible spectroscope, field emission scanning electron microscope and high resolution scanning electron microscope. The crude thermostable cellulase has been obtained from the Aspergillus fumigatus AA001 and treated with ZnO nanoparticle which shows thermal stability at 65°C up to 10 h whereas it showed pH stability in the alkaline pH range and retained its 53% of relative activity at pH 10.5. These findings may be promising in the area of biofuels production.

No MeSH data available.