Limits...
TNFα and IL-6 Responses to Particulate Matter in Vitro: Variation According to PM Size, Season, and Polycyclic Aromatic Hydrocarbon and Soil Content.

Manzano-León N, Serrano-Lomelin J, Sánchez BN, Quintana-Belmares R, Vega E, Vázquez-López I, Rojas-Bracho L, López-Villegas MT, Vadillo-Ortega F, De Vizcaya-Ruiz A, Perez IR, O'Neill MS, Osornio-Vargas AR - Environ. Health Perspect. (2015)

Bottom Line: Elevated levels of cytokine production were associated with PM10 and C2 (rainy-warm season), whereas reduced levels of cytokine production were associated with PM2.5 and C1 (dry-cold season).The results of the IL-6 assays suggested more complex interactions between PM components and particle size.These results suggest that the mixture of components in PM explains some seasonal differences in associations between health outcomes and PM in epidemiologic studies.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Investigación Básica, Instituto Nacional de Cancerología, México, DF, México.

ABSTRACT

Background: Observed seasonal differences in particulate matter (PM) associations with human health may be due to their composition and to toxicity-related seasonal interactions.

Objectives: We assessed seasonality in PM composition and in vitro PM pro-inflammatory potential using multiple PM samples.

Methods: We collected 90 weekly PM10 and PM2.5 samples during the rainy-warm and dry-cold seasons in five urban areas with different pollution sources. The elements, polycyclic aromatic hydrocarbons (PAHs), and endotoxins identified in the samples were subjected to principal component analysis (PCA). We tested the potential of the PM to induce tumor necrosis factor alpha (TNFα) and interleukin 6 (IL-6) secretion in cultured human monocytes (THP-1), and we modeled pro-inflammatory responses using the component scores.

Results: PM composition varied by size and by season. PCA identified two main components that varied by season. Combustion-related constituents (e.g., vanadium, benzo[a]pyrene, benzo[a]anthracene) mainly comprised component 1 (C1). Soil-related constituents (e.g., endotoxins, silicon, aluminum) mainly comprised component 2 (C2). PM from the rainy-warm season was high in C2. PM (particularly PM2.5) from the dry-cold season was rich in C1. Elevated levels of cytokine production were associated with PM10 and C2 (rainy-warm season), whereas reduced levels of cytokine production were associated with PM2.5 and C1 (dry-cold season). TNFα secretion was increased following exposure to PM with high (vs. low) C2 content, but TNFα secretion in response to PM was decreased following exposure to samples containing ≥ 0.1% of C1-related PAHs, regardless of C2 content. The results of the IL-6 assays suggested more complex interactions between PM components and particle size.

Conclusions: Variations in PM soil and PAH content underlie seasonal and PM size-related patterns in TNFα secretion. These results suggest that the mixture of components in PM explains some seasonal differences in associations between health outcomes and PM in epidemiologic studies.

Citation: Manzano-León N, Serrano-Lomelin J, Sánchez BN, Quintana-Belmares R, Vega E, Vázquez-López I, Rojas-Bracho L, López-Villegas MT, Vadillo-Ortega F, De Vizcaya-Ruiz A, Rosas Perez I, O'Neill MS, Osornio-Vargas AR. 2016. TNFα and IL-6 responses to particulate matter in vitro: variation according to PM size, season, and polycyclic aromatic hydrocarbon and soil content. Environ Health Perspect 124:406-412; http://dx.doi.org/10.1289/ehp.1409287.

No MeSH data available.


Related in: MedlinePlus

C1, C2 component scores plot according to PM size and season. Samples (n = 90) according to season and PM size (groups) are well differentiated in their content of C1 and C2. Rainy-warm PM10 had high concentrations of C2 and moderate concentrations of C1; dry-cold PM10 presented moderate C1 concentrations and moderate-high concentrations of C2; rainy-warm PM2.5 generally had low concentrations of C1 and C2; dry-cold PM2.5 showed high concentrations of C1 and low concentrations of C2. The zeroes on both axes correspond to the mean of all samples, and integer values are based on the component loading of the observation and the standardized value of the original variable, summed over all variables.
© Copyright Policy - public-domain
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4829995&req=5

f1: C1, C2 component scores plot according to PM size and season. Samples (n = 90) according to season and PM size (groups) are well differentiated in their content of C1 and C2. Rainy-warm PM10 had high concentrations of C2 and moderate concentrations of C1; dry-cold PM10 presented moderate C1 concentrations and moderate-high concentrations of C2; rainy-warm PM2.5 generally had low concentrations of C1 and C2; dry-cold PM2.5 showed high concentrations of C1 and low concentrations of C2. The zeroes on both axes correspond to the mean of all samples, and integer values are based on the component loading of the observation and the standardized value of the original variable, summed over all variables.

Mentions: There were four distinct patterns of distribution of C1 and C2 by size and season in the 90 PM samples: a) PM2.5 from the rainy-warm season had low C1 and C2 content; b) PM10 from the dry-cold season had approximately average C1 content and just above average C2 content; c) PM10 from the rainy-warm season had average C1 content and high C2 content; and d) PM2.5 from the dry-cold season had low C2 content and high C1 content (Figure 1).


TNFα and IL-6 Responses to Particulate Matter in Vitro: Variation According to PM Size, Season, and Polycyclic Aromatic Hydrocarbon and Soil Content.

Manzano-León N, Serrano-Lomelin J, Sánchez BN, Quintana-Belmares R, Vega E, Vázquez-López I, Rojas-Bracho L, López-Villegas MT, Vadillo-Ortega F, De Vizcaya-Ruiz A, Perez IR, O'Neill MS, Osornio-Vargas AR - Environ. Health Perspect. (2015)

C1, C2 component scores plot according to PM size and season. Samples (n = 90) according to season and PM size (groups) are well differentiated in their content of C1 and C2. Rainy-warm PM10 had high concentrations of C2 and moderate concentrations of C1; dry-cold PM10 presented moderate C1 concentrations and moderate-high concentrations of C2; rainy-warm PM2.5 generally had low concentrations of C1 and C2; dry-cold PM2.5 showed high concentrations of C1 and low concentrations of C2. The zeroes on both axes correspond to the mean of all samples, and integer values are based on the component loading of the observation and the standardized value of the original variable, summed over all variables.
© Copyright Policy - public-domain
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829995&req=5

f1: C1, C2 component scores plot according to PM size and season. Samples (n = 90) according to season and PM size (groups) are well differentiated in their content of C1 and C2. Rainy-warm PM10 had high concentrations of C2 and moderate concentrations of C1; dry-cold PM10 presented moderate C1 concentrations and moderate-high concentrations of C2; rainy-warm PM2.5 generally had low concentrations of C1 and C2; dry-cold PM2.5 showed high concentrations of C1 and low concentrations of C2. The zeroes on both axes correspond to the mean of all samples, and integer values are based on the component loading of the observation and the standardized value of the original variable, summed over all variables.
Mentions: There were four distinct patterns of distribution of C1 and C2 by size and season in the 90 PM samples: a) PM2.5 from the rainy-warm season had low C1 and C2 content; b) PM10 from the dry-cold season had approximately average C1 content and just above average C2 content; c) PM10 from the rainy-warm season had average C1 content and high C2 content; and d) PM2.5 from the dry-cold season had low C2 content and high C1 content (Figure 1).

Bottom Line: Elevated levels of cytokine production were associated with PM10 and C2 (rainy-warm season), whereas reduced levels of cytokine production were associated with PM2.5 and C1 (dry-cold season).The results of the IL-6 assays suggested more complex interactions between PM components and particle size.These results suggest that the mixture of components in PM explains some seasonal differences in associations between health outcomes and PM in epidemiologic studies.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Investigación Básica, Instituto Nacional de Cancerología, México, DF, México.

ABSTRACT

Background: Observed seasonal differences in particulate matter (PM) associations with human health may be due to their composition and to toxicity-related seasonal interactions.

Objectives: We assessed seasonality in PM composition and in vitro PM pro-inflammatory potential using multiple PM samples.

Methods: We collected 90 weekly PM10 and PM2.5 samples during the rainy-warm and dry-cold seasons in five urban areas with different pollution sources. The elements, polycyclic aromatic hydrocarbons (PAHs), and endotoxins identified in the samples were subjected to principal component analysis (PCA). We tested the potential of the PM to induce tumor necrosis factor alpha (TNFα) and interleukin 6 (IL-6) secretion in cultured human monocytes (THP-1), and we modeled pro-inflammatory responses using the component scores.

Results: PM composition varied by size and by season. PCA identified two main components that varied by season. Combustion-related constituents (e.g., vanadium, benzo[a]pyrene, benzo[a]anthracene) mainly comprised component 1 (C1). Soil-related constituents (e.g., endotoxins, silicon, aluminum) mainly comprised component 2 (C2). PM from the rainy-warm season was high in C2. PM (particularly PM2.5) from the dry-cold season was rich in C1. Elevated levels of cytokine production were associated with PM10 and C2 (rainy-warm season), whereas reduced levels of cytokine production were associated with PM2.5 and C1 (dry-cold season). TNFα secretion was increased following exposure to PM with high (vs. low) C2 content, but TNFα secretion in response to PM was decreased following exposure to samples containing ≥ 0.1% of C1-related PAHs, regardless of C2 content. The results of the IL-6 assays suggested more complex interactions between PM components and particle size.

Conclusions: Variations in PM soil and PAH content underlie seasonal and PM size-related patterns in TNFα secretion. These results suggest that the mixture of components in PM explains some seasonal differences in associations between health outcomes and PM in epidemiologic studies.

Citation: Manzano-León N, Serrano-Lomelin J, Sánchez BN, Quintana-Belmares R, Vega E, Vázquez-López I, Rojas-Bracho L, López-Villegas MT, Vadillo-Ortega F, De Vizcaya-Ruiz A, Rosas Perez I, O'Neill MS, Osornio-Vargas AR. 2016. TNFα and IL-6 responses to particulate matter in vitro: variation according to PM size, season, and polycyclic aromatic hydrocarbon and soil content. Environ Health Perspect 124:406-412; http://dx.doi.org/10.1289/ehp.1409287.

No MeSH data available.


Related in: MedlinePlus