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Systematics and biology of some species of Micrurapteryx Spuler (Lepidoptera, Gracillariidae) from the Holarctic Region, with re-description of M. caraganella (Hering) from Siberia.

Kirichenko N, Triberti P, Mutanen M, Magnoux E, Landry JF, Lopez-Vaamonde C - Zookeys (2016)

Bottom Line: In addition, based on both morphological and molecular evidence as well as examination of type specimens, the North American Parectopa occulta Braun, 1922 and Parectopa albicostella Braun, 1925 are transferred to Micrurapteryx as Micrurapteryx occulta (Braun, 1922), comb. n. with albicostella as its junior synonym (syn. n.).Characters used to distinguish Micrurapteryx from Parectopa are presented and illustrated.These findings provide another example of the potential of DNA barcoding to reveal overlooked species and illuminate nomenclatural problems.

View Article: PubMed Central - HTML - PubMed

Affiliation: Sukachev Institute of Forest SB RAS, Akademgorodok 50/28, 660036, Krasnoyarsk, Russia; Siberian Federal University, 79 Svobodny pr., 660041, Krasnoyarsk, Russia; INRA, UR0633 Zoologie Forestière, F-45075 Orléans, France.

ABSTRACT
During a DNA barcoding campaign of leaf-mining insects from Siberia, a genetically divergent lineage of a gracillariid belonging to the genus Micrurapteryx was discovered, whose larvae developed on Caragana Fabr. and Medicago L. (Fabaceae). Specimens from Siberia showed similar external morphology to the Palearctic Micrurapteryx gradatella and the Nearctic Parectopa occulta but differed in male genitalia, DNA barcodes, and nuclear genes histone H3 and 28S. Members of this lineage are re-described here as Micrurapteryx caraganella (Hering, 1957), comb. n., an available name published with only a brief description of its larva and leaf mine. Micrurapteryx caraganella is widely distributed throughout Siberia, from Tyumen oblast in the West to Transbaikalia in the East. Occasionally it may severely affect its main host, Caragana arborescens Lam. This species has been confused in the past with Micrurapteryx gradatella in Siberia, but field observations confirm that Micrurapteryx gradatella exists in Siberia and is sympatric with Micrurapteryx caraganella, at least in the Krasnoyarsk region, where it feeds on different host plants (Vicia amoena Fisch. and Vicia sp.). In addition, based on both morphological and molecular evidence as well as examination of type specimens, the North American Parectopa occulta Braun, 1922 and Parectopa albicostella Braun, 1925 are transferred to Micrurapteryx as Micrurapteryx occulta (Braun, 1922), comb. n. with albicostella as its junior synonym (syn. n.). Characters used to distinguish Micrurapteryx from Parectopa are presented and illustrated. These findings provide another example of the potential of DNA barcoding to reveal overlooked species and illuminate nomenclatural problems.

No MeSH data available.


Related in: MedlinePlus

The Neighbor-joining trees, based on fragment of nuclear genes histone H3 and 28S, generated under the K2P nucleotide substitution model, of the studied taxa. Branch lengths represent genetic K2P divergences between the taxa according to the scale. Host plants are indicated for those specimens, which were bred from mines. Genetic divergence between Micrurapteryxcaraganella and Micrurapteryxgradatella is due to three mutations in the histoneH3 gene (0.92% interspecific distance) and two mutations in the 28S gene (0.20 % interspecific distance).
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Figure 2: The Neighbor-joining trees, based on fragment of nuclear genes histone H3 and 28S, generated under the K2P nucleotide substitution model, of the studied taxa. Branch lengths represent genetic K2P divergences between the taxa according to the scale. Host plants are indicated for those specimens, which were bred from mines. Genetic divergence between Micrurapteryxcaraganella and Micrurapteryxgradatella is due to three mutations in the histoneH3 gene (0.92% interspecific distance) and two mutations in the 28S gene (0.20 % interspecific distance).

Mentions: We obtained sequences of the nuclear gene histone H3 and 28S rRNA D1-D3 for 23 specimens (17 specimens of Micrurapteryxcaraganella and 6 specimens of Micrurapteryxgradatella, Table 1). Both H3 and 28S unequivocally delimit two distinct species with 3 and 2 diagnostic nucleotide substitutions respectively (Fig. 2; Suppl. material 1: Table S4). Sequencing these two genes confirm the presence of Micrurapteryxcaraganella on both Caragana and Medicago in Siberia. No evidence of mitochondrial introgression between Micrurapteryxcaraganella and Micrurapteryxgradatella was recorded.


Systematics and biology of some species of Micrurapteryx Spuler (Lepidoptera, Gracillariidae) from the Holarctic Region, with re-description of M. caraganella (Hering) from Siberia.

Kirichenko N, Triberti P, Mutanen M, Magnoux E, Landry JF, Lopez-Vaamonde C - Zookeys (2016)

The Neighbor-joining trees, based on fragment of nuclear genes histone H3 and 28S, generated under the K2P nucleotide substitution model, of the studied taxa. Branch lengths represent genetic K2P divergences between the taxa according to the scale. Host plants are indicated for those specimens, which were bred from mines. Genetic divergence between Micrurapteryxcaraganella and Micrurapteryxgradatella is due to three mutations in the histoneH3 gene (0.92% interspecific distance) and two mutations in the 28S gene (0.20 % interspecific distance).
© Copyright Policy - creative-commons-attribution
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829971&req=5

Figure 2: The Neighbor-joining trees, based on fragment of nuclear genes histone H3 and 28S, generated under the K2P nucleotide substitution model, of the studied taxa. Branch lengths represent genetic K2P divergences between the taxa according to the scale. Host plants are indicated for those specimens, which were bred from mines. Genetic divergence between Micrurapteryxcaraganella and Micrurapteryxgradatella is due to three mutations in the histoneH3 gene (0.92% interspecific distance) and two mutations in the 28S gene (0.20 % interspecific distance).
Mentions: We obtained sequences of the nuclear gene histone H3 and 28S rRNA D1-D3 for 23 specimens (17 specimens of Micrurapteryxcaraganella and 6 specimens of Micrurapteryxgradatella, Table 1). Both H3 and 28S unequivocally delimit two distinct species with 3 and 2 diagnostic nucleotide substitutions respectively (Fig. 2; Suppl. material 1: Table S4). Sequencing these two genes confirm the presence of Micrurapteryxcaraganella on both Caragana and Medicago in Siberia. No evidence of mitochondrial introgression between Micrurapteryxcaraganella and Micrurapteryxgradatella was recorded.

Bottom Line: In addition, based on both morphological and molecular evidence as well as examination of type specimens, the North American Parectopa occulta Braun, 1922 and Parectopa albicostella Braun, 1925 are transferred to Micrurapteryx as Micrurapteryx occulta (Braun, 1922), comb. n. with albicostella as its junior synonym (syn. n.).Characters used to distinguish Micrurapteryx from Parectopa are presented and illustrated.These findings provide another example of the potential of DNA barcoding to reveal overlooked species and illuminate nomenclatural problems.

View Article: PubMed Central - HTML - PubMed

Affiliation: Sukachev Institute of Forest SB RAS, Akademgorodok 50/28, 660036, Krasnoyarsk, Russia; Siberian Federal University, 79 Svobodny pr., 660041, Krasnoyarsk, Russia; INRA, UR0633 Zoologie Forestière, F-45075 Orléans, France.

ABSTRACT
During a DNA barcoding campaign of leaf-mining insects from Siberia, a genetically divergent lineage of a gracillariid belonging to the genus Micrurapteryx was discovered, whose larvae developed on Caragana Fabr. and Medicago L. (Fabaceae). Specimens from Siberia showed similar external morphology to the Palearctic Micrurapteryx gradatella and the Nearctic Parectopa occulta but differed in male genitalia, DNA barcodes, and nuclear genes histone H3 and 28S. Members of this lineage are re-described here as Micrurapteryx caraganella (Hering, 1957), comb. n., an available name published with only a brief description of its larva and leaf mine. Micrurapteryx caraganella is widely distributed throughout Siberia, from Tyumen oblast in the West to Transbaikalia in the East. Occasionally it may severely affect its main host, Caragana arborescens Lam. This species has been confused in the past with Micrurapteryx gradatella in Siberia, but field observations confirm that Micrurapteryx gradatella exists in Siberia and is sympatric with Micrurapteryx caraganella, at least in the Krasnoyarsk region, where it feeds on different host plants (Vicia amoena Fisch. and Vicia sp.). In addition, based on both morphological and molecular evidence as well as examination of type specimens, the North American Parectopa occulta Braun, 1922 and Parectopa albicostella Braun, 1925 are transferred to Micrurapteryx as Micrurapteryx occulta (Braun, 1922), comb. n. with albicostella as its junior synonym (syn. n.). Characters used to distinguish Micrurapteryx from Parectopa are presented and illustrated. These findings provide another example of the potential of DNA barcoding to reveal overlooked species and illuminate nomenclatural problems.

No MeSH data available.


Related in: MedlinePlus