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25-Hydroxyvitamin D in pregnancy and genome wide cord blood DNA methylation in two pregnancy cohorts (MoBa and ALSPAC).

Suderman M, Stene LC, Bohlin J, Page CM, Holvik K, Parr CL, Magnus MC, Håberg SE, Joubert BR, Wu MC, London SJ, Relton C, Nystad W - J. Steroid Biochem. Mol. Biol. (2016)

Bottom Line: Further exploration of a set of CpG sites in the proximity of four a priori defined candidate genes (CYP24A1, CYP27B1, CYP27A1 and CYP2R1) did not result in any associations with FDR<0.05 (56 tests).In this large genome wide assessment of the potential influence of maternal vitamin D status on DNA methylation, we did not find any convincing associations in 1416 newborns.If true associations do exist, their identification might require much larger consortium studies, expanded genomic coverage, investigation of alternative cell types or measurements of 25-hydroxyvitamin D at different gestational time points.

View Article: PubMed Central - PubMed

Affiliation: MRC Integrative Epidemiology Unit, University of Bristol, Oakfield House, Oakfield Grove, Clifton, Bristol BS8 2BN, UK. Electronic address: matthew.suderman@bristol.ac.uk.

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ALSPAC regression coefficient estimate confidence intervals typically contain zero. Estimated slope between methylation levels of offspring CpG sites and maternal vitamin D levels from the crude model for 4 candidate genes in the ALSPAC study. The horizontal axis represents the genomic positions of the CpGs, while the vertical axes represent slope estimates. The vertical lines represent ±1.96 × estimated standard error for the regression coefficient.
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fig0020: ALSPAC regression coefficient estimate confidence intervals typically contain zero. Estimated slope between methylation levels of offspring CpG sites and maternal vitamin D levels from the crude model for 4 candidate genes in the ALSPAC study. The horizontal axis represents the genomic positions of the CpGs, while the vertical axes represent slope estimates. The vertical lines represent ±1.96 × estimated standard error for the regression coefficient.

Mentions: Furthermore, detailed analysis of CpG’s linked to the four a priori defined candidate genes (CYP24A1, CYP27B1, CYP27A1 and CYP2R1) yielded weak associations with very small regression coefficient estimates (Fig. 3; FDR > 0.6 for 56 tests; see Supplementary information File 2). Repeating the analysis within the ALSPAC cohort, there were similarly only weak associations between maternal 25(OH)D and cord blood DNA methylation among 597 mother and child pairs both at the genome wide and at the four candidate loci (Fig. 4).


25-Hydroxyvitamin D in pregnancy and genome wide cord blood DNA methylation in two pregnancy cohorts (MoBa and ALSPAC).

Suderman M, Stene LC, Bohlin J, Page CM, Holvik K, Parr CL, Magnus MC, Håberg SE, Joubert BR, Wu MC, London SJ, Relton C, Nystad W - J. Steroid Biochem. Mol. Biol. (2016)

ALSPAC regression coefficient estimate confidence intervals typically contain zero. Estimated slope between methylation levels of offspring CpG sites and maternal vitamin D levels from the crude model for 4 candidate genes in the ALSPAC study. The horizontal axis represents the genomic positions of the CpGs, while the vertical axes represent slope estimates. The vertical lines represent ±1.96 × estimated standard error for the regression coefficient.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829940&req=5

fig0020: ALSPAC regression coefficient estimate confidence intervals typically contain zero. Estimated slope between methylation levels of offspring CpG sites and maternal vitamin D levels from the crude model for 4 candidate genes in the ALSPAC study. The horizontal axis represents the genomic positions of the CpGs, while the vertical axes represent slope estimates. The vertical lines represent ±1.96 × estimated standard error for the regression coefficient.
Mentions: Furthermore, detailed analysis of CpG’s linked to the four a priori defined candidate genes (CYP24A1, CYP27B1, CYP27A1 and CYP2R1) yielded weak associations with very small regression coefficient estimates (Fig. 3; FDR > 0.6 for 56 tests; see Supplementary information File 2). Repeating the analysis within the ALSPAC cohort, there were similarly only weak associations between maternal 25(OH)D and cord blood DNA methylation among 597 mother and child pairs both at the genome wide and at the four candidate loci (Fig. 4).

Bottom Line: Further exploration of a set of CpG sites in the proximity of four a priori defined candidate genes (CYP24A1, CYP27B1, CYP27A1 and CYP2R1) did not result in any associations with FDR<0.05 (56 tests).In this large genome wide assessment of the potential influence of maternal vitamin D status on DNA methylation, we did not find any convincing associations in 1416 newborns.If true associations do exist, their identification might require much larger consortium studies, expanded genomic coverage, investigation of alternative cell types or measurements of 25-hydroxyvitamin D at different gestational time points.

View Article: PubMed Central - PubMed

Affiliation: MRC Integrative Epidemiology Unit, University of Bristol, Oakfield House, Oakfield Grove, Clifton, Bristol BS8 2BN, UK. Electronic address: matthew.suderman@bristol.ac.uk.

Show MeSH
Related in: MedlinePlus