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High expression of microRNA-183/182/96 cluster as a prognostic biomarker for breast cancer.

Song C, Zhang L, Wang J, Huang Z, Li X, Wu M, Li S, Tang H, Xie X - Sci Rep (2016)

Bottom Line: More sensitive and effective diagnostic markers for the detection of breast cancer are urgently needed.The miR-183/182/96 cluster levels were significantly higher in breast cancer tissues than in control tissues.An increased miR-183/182/96 cluster level was correlated with local relapse, distant metastasis and poor clinical outcomes.

View Article: PubMed Central - PubMed

Affiliation: Department of Breast Oncology, Sun Yat-Sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangdong, China.

ABSTRACT
More sensitive and effective diagnostic markers for the detection of breast cancer are urgently needed. The microRNA-183/182/96 cluster has been reported to be involved in tumorigenesis and progression in a variety of cancers, and it is a promising cancer prognostic biomarker. The goal of this study was to determine the expression levels of the miR-183/182/96 cluster in breast cancer tissues and evaluate its prognostic role in breast cancer. Real-time quantitative polymerase chain reaction analysis (qRT-PCR) was used to detect the expression levels of the miR-183/182/96 cluster in 41 breast cancer tissues and adjacent normal tissues (control tissues) and also in different mammary cell lines. In situ hybridization (ISH) of the miR-183/182/96 cluster on 131 tissue microarrays (TMAs) was used to statistically analyze its prognostic role. The miR-183/182/96 cluster levels were significantly higher in breast cancer tissues than in control tissues. The miR-183/182/96 cluster was also upregulated in human breast cancer cell lines. An increased miR-183/182/96 cluster level was correlated with local relapse, distant metastasis and poor clinical outcomes. Our findings improve our understanding of the expression level of the miR-183/182/96 cluster in breast cancer and clarify the role of the miR-183/182/96 cluster as a novel prognostic biomarker for breast cancer.

No MeSH data available.


Related in: MedlinePlus

Increased miR-183/182/96 level was correlated with poor prognosis.(A) OS curves for 131 patients with low or high miR-183, miR-182, miR-96 and miR-183/182/96 cluster expression. Kaplan-Meier and log-rank analyses were used. High levels of the miR-183/182/96 cluster were markedly correlated with shorter overall survival. (B) DFS curves for 131 patients with low or high miR-183, miR-182, miR-96 and miR-183/182/96 cluster expression. A high level of the miR-183/182/96 cluster was markedly correlated with shorter disease-free survival. All data are shown as the mean ± SE ***P < 0.01.
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f3: Increased miR-183/182/96 level was correlated with poor prognosis.(A) OS curves for 131 patients with low or high miR-183, miR-182, miR-96 and miR-183/182/96 cluster expression. Kaplan-Meier and log-rank analyses were used. High levels of the miR-183/182/96 cluster were markedly correlated with shorter overall survival. (B) DFS curves for 131 patients with low or high miR-183, miR-182, miR-96 and miR-183/182/96 cluster expression. A high level of the miR-183/182/96 cluster was markedly correlated with shorter disease-free survival. All data are shown as the mean ± SE ***P < 0.01.

Mentions: The results above revealed that miR-183/182/96 level was higher in breast cancer and was positively correlated with some clinical pathological parameters, indicating that miR-183/182/96 may be vital for the pathogenesis of breast cancer. To further explore the prognostic significance of miR-183/182/96, we used Kaplan-Meier survival analysis to generate patient overall survival (OS) and disease-free survival (DFS) curves. The results demonstrated that patients with higher miR-183, miR-182 and miR-96 expression levels had shorter mean OS and DFS than patients with lower expression levels (P = 0.000, 0.000 and 0.022 for OS, respectively and P = 0.000, 0.004 and 0.011 for DFS, respectively, Fig. 3A,B). Further more, when the expression levels of all three members of the miR-183/182/96 cluster were high, OS and DFS were much shorter than when zero, one, or two members of the miR-183/182/96 cluster were highly expressed (all P for OS = 0.000 and all P for DFS = 0.004). These results demonstrated that miR-183/182/96 expression was significantly associated with patient OS and DFS. The miR-183/182/96 cluster is a potential prognostic biomarker for breast cancer.


High expression of microRNA-183/182/96 cluster as a prognostic biomarker for breast cancer.

Song C, Zhang L, Wang J, Huang Z, Li X, Wu M, Li S, Tang H, Xie X - Sci Rep (2016)

Increased miR-183/182/96 level was correlated with poor prognosis.(A) OS curves for 131 patients with low or high miR-183, miR-182, miR-96 and miR-183/182/96 cluster expression. Kaplan-Meier and log-rank analyses were used. High levels of the miR-183/182/96 cluster were markedly correlated with shorter overall survival. (B) DFS curves for 131 patients with low or high miR-183, miR-182, miR-96 and miR-183/182/96 cluster expression. A high level of the miR-183/182/96 cluster was markedly correlated with shorter disease-free survival. All data are shown as the mean ± SE ***P < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829912&req=5

f3: Increased miR-183/182/96 level was correlated with poor prognosis.(A) OS curves for 131 patients with low or high miR-183, miR-182, miR-96 and miR-183/182/96 cluster expression. Kaplan-Meier and log-rank analyses were used. High levels of the miR-183/182/96 cluster were markedly correlated with shorter overall survival. (B) DFS curves for 131 patients with low or high miR-183, miR-182, miR-96 and miR-183/182/96 cluster expression. A high level of the miR-183/182/96 cluster was markedly correlated with shorter disease-free survival. All data are shown as the mean ± SE ***P < 0.01.
Mentions: The results above revealed that miR-183/182/96 level was higher in breast cancer and was positively correlated with some clinical pathological parameters, indicating that miR-183/182/96 may be vital for the pathogenesis of breast cancer. To further explore the prognostic significance of miR-183/182/96, we used Kaplan-Meier survival analysis to generate patient overall survival (OS) and disease-free survival (DFS) curves. The results demonstrated that patients with higher miR-183, miR-182 and miR-96 expression levels had shorter mean OS and DFS than patients with lower expression levels (P = 0.000, 0.000 and 0.022 for OS, respectively and P = 0.000, 0.004 and 0.011 for DFS, respectively, Fig. 3A,B). Further more, when the expression levels of all three members of the miR-183/182/96 cluster were high, OS and DFS were much shorter than when zero, one, or two members of the miR-183/182/96 cluster were highly expressed (all P for OS = 0.000 and all P for DFS = 0.004). These results demonstrated that miR-183/182/96 expression was significantly associated with patient OS and DFS. The miR-183/182/96 cluster is a potential prognostic biomarker for breast cancer.

Bottom Line: More sensitive and effective diagnostic markers for the detection of breast cancer are urgently needed.The miR-183/182/96 cluster levels were significantly higher in breast cancer tissues than in control tissues.An increased miR-183/182/96 cluster level was correlated with local relapse, distant metastasis and poor clinical outcomes.

View Article: PubMed Central - PubMed

Affiliation: Department of Breast Oncology, Sun Yat-Sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, Guangdong, China.

ABSTRACT
More sensitive and effective diagnostic markers for the detection of breast cancer are urgently needed. The microRNA-183/182/96 cluster has been reported to be involved in tumorigenesis and progression in a variety of cancers, and it is a promising cancer prognostic biomarker. The goal of this study was to determine the expression levels of the miR-183/182/96 cluster in breast cancer tissues and evaluate its prognostic role in breast cancer. Real-time quantitative polymerase chain reaction analysis (qRT-PCR) was used to detect the expression levels of the miR-183/182/96 cluster in 41 breast cancer tissues and adjacent normal tissues (control tissues) and also in different mammary cell lines. In situ hybridization (ISH) of the miR-183/182/96 cluster on 131 tissue microarrays (TMAs) was used to statistically analyze its prognostic role. The miR-183/182/96 cluster levels were significantly higher in breast cancer tissues than in control tissues. The miR-183/182/96 cluster was also upregulated in human breast cancer cell lines. An increased miR-183/182/96 cluster level was correlated with local relapse, distant metastasis and poor clinical outcomes. Our findings improve our understanding of the expression level of the miR-183/182/96 cluster in breast cancer and clarify the role of the miR-183/182/96 cluster as a novel prognostic biomarker for breast cancer.

No MeSH data available.


Related in: MedlinePlus