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Morphological and molecular characterisation, and phylogenetic position of X. browni sp. n., X. penevi sp. n. and two known species of Xiphinema americanum-group (Nematoda, Longidoridae).

Lazarova S, Peneva V, Kumari S - Zookeys (2016)

Bottom Line: The first species is most similar to Xiphinema parasimile and is a member of Xiphinema simile species complex.Also, a separate "Xiphinema simile-subgroup", outside the Xiphinema pachtaicum-subgroup and so far consisting only of the parthenogenetic species Xiphinema simile, Xiphinema parasimile, Xiphinema browni sp. n. and probably Xiphinema vallense was formed.New primers for amplification and sequencing of part of the nad4 mitochondrial gene were designed and used.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Animal Biodiversity and Resources, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences 2, Gagarin Street, 1113 Sofia, Bulgaria.

ABSTRACT
Using ribosomal (18S, ITS1, ITS2, D2-D3 expansion segments of 28S rDNA) and mitochondrial (partial cox1 and nad4) DNA markers in a study of several populations of Xiphinema americanum-group from Europe and Morocco, two cryptic species Xiphinema browni sp. n. (formerly reported as Xiphinema pachtaicum) and Xiphinema penevi sp. n. were revealed. The species are described, illustrated and their phylogenetic relationships discussed. The first species is most similar to Xiphinema parasimile and is a member of Xiphinema simile species complex. The phylogenetic reconstructions inferred from three molecular markers (18S, D2-D3 28S rDNA and cox1) showed that Xiphinema penevi sp. n. is part of Xiphinema pachtaicum-subgroup and is closely related to Xiphinema incertum, Xiphinema pachtaicum, Xiphinema parapachydermum, Xiphinema plesiopachtaicum, Xiphinema astaregiense and Xiphinema pachydermum. Also, a separate "Xiphinema simile-subgroup", outside the Xiphinema pachtaicum-subgroup and so far consisting only of the parthenogenetic species Xiphinema simile, Xiphinema parasimile, Xiphinema browni sp. n. and probably Xiphinema vallense was formed. New primers for amplification and sequencing of part of the nad4 mitochondrial gene were designed and used.

No MeSH data available.


Hypothesis of the phylogenetic relationships of Xiphinemabrowni sp. n. and Xiphinemaparasimile based on cox1 inferred from a Bayesian analysis using GTR+G model and Xiphinemaitaliae Mayl, 1953 and Xiphinemadiversicaudatum (Micoletzky, 1927), Thorne, 1939 as an outgroup. Posterior probabilities higher than 0.8 are presented.
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Figure 12: Hypothesis of the phylogenetic relationships of Xiphinemabrowni sp. n. and Xiphinemaparasimile based on cox1 inferred from a Bayesian analysis using GTR+G model and Xiphinemaitaliae Mayl, 1953 and Xiphinemadiversicaudatum (Micoletzky, 1927), Thorne, 1939 as an outgroup. Posterior probabilities higher than 0.8 are presented.

Mentions: In all three phylogeny reconstructions (18S, D2-D3 and cox1) Xiphinemaparasimile from Bulgaria was a sister species of Xiphinemabrowni sp. n. and both species were part of a well supported clade with other European populations of Xiphinemasimile (Figs 10–12). The recently described species Xiphinemavallense Archidona-Yuste, Navas-Cortes, Cantalapiedra-Navarrete, Palomares-Rius & Castillo, 2016 presented only with D2-D3 and ITS1 rDNA sequences seems also to be evolutionary very closely related (Figs 11 and 13), however amplifying additional sequences for other molecular markers (e.g. 18S and cox1) could help to better clarify its relationships. The position of the new species in the phylogeny trees based on ITS1 and ITS2 sequences was unstable (Figs 13 and 14). The analyses resulted in various tree topologies when using different alignment algorithms and reconstruction methods (ML and BI) and because of the absence of homologous sequences from closely related species. In most cases Xiphinemabrowni sp. n. was part of a clade of European Xiphinemaamericanum-group species considered as group II in a previous publication (Archidona-Yuste et al. 2016). Due to insufficient number of nad4 sequences of species belonging to the Xiphinemaamericanum-group at NCBI no phylogenetic reconstructions are presented.


Morphological and molecular characterisation, and phylogenetic position of X. browni sp. n., X. penevi sp. n. and two known species of Xiphinema americanum-group (Nematoda, Longidoridae).

Lazarova S, Peneva V, Kumari S - Zookeys (2016)

Hypothesis of the phylogenetic relationships of Xiphinemabrowni sp. n. and Xiphinemaparasimile based on cox1 inferred from a Bayesian analysis using GTR+G model and Xiphinemaitaliae Mayl, 1953 and Xiphinemadiversicaudatum (Micoletzky, 1927), Thorne, 1939 as an outgroup. Posterior probabilities higher than 0.8 are presented.
© Copyright Policy - creative-commons-attribution
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829900&req=5

Figure 12: Hypothesis of the phylogenetic relationships of Xiphinemabrowni sp. n. and Xiphinemaparasimile based on cox1 inferred from a Bayesian analysis using GTR+G model and Xiphinemaitaliae Mayl, 1953 and Xiphinemadiversicaudatum (Micoletzky, 1927), Thorne, 1939 as an outgroup. Posterior probabilities higher than 0.8 are presented.
Mentions: In all three phylogeny reconstructions (18S, D2-D3 and cox1) Xiphinemaparasimile from Bulgaria was a sister species of Xiphinemabrowni sp. n. and both species were part of a well supported clade with other European populations of Xiphinemasimile (Figs 10–12). The recently described species Xiphinemavallense Archidona-Yuste, Navas-Cortes, Cantalapiedra-Navarrete, Palomares-Rius & Castillo, 2016 presented only with D2-D3 and ITS1 rDNA sequences seems also to be evolutionary very closely related (Figs 11 and 13), however amplifying additional sequences for other molecular markers (e.g. 18S and cox1) could help to better clarify its relationships. The position of the new species in the phylogeny trees based on ITS1 and ITS2 sequences was unstable (Figs 13 and 14). The analyses resulted in various tree topologies when using different alignment algorithms and reconstruction methods (ML and BI) and because of the absence of homologous sequences from closely related species. In most cases Xiphinemabrowni sp. n. was part of a clade of European Xiphinemaamericanum-group species considered as group II in a previous publication (Archidona-Yuste et al. 2016). Due to insufficient number of nad4 sequences of species belonging to the Xiphinemaamericanum-group at NCBI no phylogenetic reconstructions are presented.

Bottom Line: The first species is most similar to Xiphinema parasimile and is a member of Xiphinema simile species complex.Also, a separate "Xiphinema simile-subgroup", outside the Xiphinema pachtaicum-subgroup and so far consisting only of the parthenogenetic species Xiphinema simile, Xiphinema parasimile, Xiphinema browni sp. n. and probably Xiphinema vallense was formed.New primers for amplification and sequencing of part of the nad4 mitochondrial gene were designed and used.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Animal Biodiversity and Resources, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences 2, Gagarin Street, 1113 Sofia, Bulgaria.

ABSTRACT
Using ribosomal (18S, ITS1, ITS2, D2-D3 expansion segments of 28S rDNA) and mitochondrial (partial cox1 and nad4) DNA markers in a study of several populations of Xiphinema americanum-group from Europe and Morocco, two cryptic species Xiphinema browni sp. n. (formerly reported as Xiphinema pachtaicum) and Xiphinema penevi sp. n. were revealed. The species are described, illustrated and their phylogenetic relationships discussed. The first species is most similar to Xiphinema parasimile and is a member of Xiphinema simile species complex. The phylogenetic reconstructions inferred from three molecular markers (18S, D2-D3 28S rDNA and cox1) showed that Xiphinema penevi sp. n. is part of Xiphinema pachtaicum-subgroup and is closely related to Xiphinema incertum, Xiphinema pachtaicum, Xiphinema parapachydermum, Xiphinema plesiopachtaicum, Xiphinema astaregiense and Xiphinema pachydermum. Also, a separate "Xiphinema simile-subgroup", outside the Xiphinema pachtaicum-subgroup and so far consisting only of the parthenogenetic species Xiphinema simile, Xiphinema parasimile, Xiphinema browni sp. n. and probably Xiphinema vallense was formed. New primers for amplification and sequencing of part of the nad4 mitochondrial gene were designed and used.

No MeSH data available.