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Protective effect of chorioamnionitis on the development of bronchopulmonary dysplasia triggered by postnatal systemic inflammation in neonatal rats.

Choi CW, Lee J, Oh JY, Lee SH, Lee HJ, Kim BI - Pediatr. Res. (2015)

Bottom Line: LPS significantly negated the detrimental effects of postnatal i.p.Preceding exposure to i.a.LPS protects the lungs against BPD triggered by postnatal systemic inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Seoul National University College of Medicine, Seoul, Republic of Korea.

ABSTRACT

Background: Prenatal or postnatal systemic inflammation can contribute to the development of bronchopulmonary dysplasia (BPD). We investigated whether prenatal intra-amniotic (i.a.) inflammation or early postnatal systemic inflammation can induce BPD in a rat model.

Methods: One microgram of lipopolysaccharide (LPS) or vehicle was injected into the amniotic sacs 2 d before delivery (E20). After birth, 0.25 mg/kg of LPS or vehicle was injected into the peritoneum of pups on postnatal day (P)1, P3, and P5. On P7 and P14, peripheral blood (PB), bronchoalveolar lavage fluid (BALF), and lung tissue were obtained and analyzed.

Results: Postnatal i.p. injections of LPS significantly increased neutrophil counts in PB and BALF on P7 and P14. Similarly, proinflammatory cytokine and angiogenic factor transcript levels were increased in the lung by i.p. LPS on P7. Alveolar and pulmonary vascular development was markedly disrupted by i.p. LPS on P14. However, pretreatment with i.a. LPS significantly negated the detrimental effects of postnatal i.p. LPS on PB and BALF neutrophil counts and on lung proinflammatory cytokine expression and histopathological changes.

Conclusion: Exposure to early postnatal systemic LPS induces BPD, an arrest in alveolarization, in neonatal rats. Preceding exposure to i.a. LPS protects the lungs against BPD triggered by postnatal systemic inflammation.

No MeSH data available.


Related in: MedlinePlus

Bronchoalveolar lavage fluid and peripheral blood white blood cell and neutrophil counts. (a, b, d, e) Gray bars denote P7 and black bars P14. (a) The total BALF WBC counts were higher in the i.p. LPS-treated groups than in the i.p. vehicle-treated groups on P7 and P14. (b) The V+LPS group had higher BALF neutrophil counts than in other groups on P7 and in the V+V group on P14. (c) Representative light microscopic images of the cytospin BALF samples on P7. A marked increase in the number of neutrophils in the BALF cytospin sample from the V+LPS group is observed compared with the other groups. The arrows indicate neutrophils. Diff-Quik (Sysmex, Kobe, Japan) stained. Original magnification ×400. The bar indicates 50 μm. (d, e) The total PB WBC and neutrophil counts were highest in the V+LPS group on P7. On P14, the total WBC and neutrophil counts in PB were reduced compared with P7, and the V+V group had the highest number of total WBC and neutrophil counts in PB. Group abbreviation: V+V, i.a. vehicle followed by i.p. vehicle-treated group; LPS+V, i.a. LPS followed by i.p. vehicle-treated group; V+LPS, i.a. vehicle followed by i.p. LPS-treated group; LPS+LPS, i.a. LPS followed by i.p. LPS-treated group. The data are the mean ± SEM (N = 5–6 in each group). *P < 0.05 vs. V+V, ** P < 0.05 vs. LPS+V, †P < 0.05 vs. LPS+LPS, ‡P < 0.05 vs. V+LPS. BALF, bronchoalveolar lavage fluid; PB, peripheral blood; V, vehicle; WBC, white blood cell.
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fig3: Bronchoalveolar lavage fluid and peripheral blood white blood cell and neutrophil counts. (a, b, d, e) Gray bars denote P7 and black bars P14. (a) The total BALF WBC counts were higher in the i.p. LPS-treated groups than in the i.p. vehicle-treated groups on P7 and P14. (b) The V+LPS group had higher BALF neutrophil counts than in other groups on P7 and in the V+V group on P14. (c) Representative light microscopic images of the cytospin BALF samples on P7. A marked increase in the number of neutrophils in the BALF cytospin sample from the V+LPS group is observed compared with the other groups. The arrows indicate neutrophils. Diff-Quik (Sysmex, Kobe, Japan) stained. Original magnification ×400. The bar indicates 50 μm. (d, e) The total PB WBC and neutrophil counts were highest in the V+LPS group on P7. On P14, the total WBC and neutrophil counts in PB were reduced compared with P7, and the V+V group had the highest number of total WBC and neutrophil counts in PB. Group abbreviation: V+V, i.a. vehicle followed by i.p. vehicle-treated group; LPS+V, i.a. LPS followed by i.p. vehicle-treated group; V+LPS, i.a. vehicle followed by i.p. LPS-treated group; LPS+LPS, i.a. LPS followed by i.p. LPS-treated group. The data are the mean ± SEM (N = 5–6 in each group). *P < 0.05 vs. V+V, ** P < 0.05 vs. LPS+V, †P < 0.05 vs. LPS+LPS, ‡P < 0.05 vs. V+LPS. BALF, bronchoalveolar lavage fluid; PB, peripheral blood; V, vehicle; WBC, white blood cell.

Mentions: Postnatal i.p. LPS significantly increased the total white blood cell (WBC) counts in the bronchoalveolar lavage fluid (BALF) at P7 and P14 (Figure 3a). The total BALF neutrophil counts on P7 were significantly higher in the V+LPS group compared with the other groups (Figure 3b,c). The total BALF neutrophil counts were markedly decreased in all groups on P14 compared with those at P7. However, the V+LPS group had a significantly higher number of BALF neutrophils than the V+V group. Similar findings were observed with the total WBC or neutrophil counts in peripheral blood (PB) on P7 (Figure 3d,e). Notably, the V+V group had the highest total PB WBC and neutrophil counts on P14 among the groups.


Protective effect of chorioamnionitis on the development of bronchopulmonary dysplasia triggered by postnatal systemic inflammation in neonatal rats.

Choi CW, Lee J, Oh JY, Lee SH, Lee HJ, Kim BI - Pediatr. Res. (2015)

Bronchoalveolar lavage fluid and peripheral blood white blood cell and neutrophil counts. (a, b, d, e) Gray bars denote P7 and black bars P14. (a) The total BALF WBC counts were higher in the i.p. LPS-treated groups than in the i.p. vehicle-treated groups on P7 and P14. (b) The V+LPS group had higher BALF neutrophil counts than in other groups on P7 and in the V+V group on P14. (c) Representative light microscopic images of the cytospin BALF samples on P7. A marked increase in the number of neutrophils in the BALF cytospin sample from the V+LPS group is observed compared with the other groups. The arrows indicate neutrophils. Diff-Quik (Sysmex, Kobe, Japan) stained. Original magnification ×400. The bar indicates 50 μm. (d, e) The total PB WBC and neutrophil counts were highest in the V+LPS group on P7. On P14, the total WBC and neutrophil counts in PB were reduced compared with P7, and the V+V group had the highest number of total WBC and neutrophil counts in PB. Group abbreviation: V+V, i.a. vehicle followed by i.p. vehicle-treated group; LPS+V, i.a. LPS followed by i.p. vehicle-treated group; V+LPS, i.a. vehicle followed by i.p. LPS-treated group; LPS+LPS, i.a. LPS followed by i.p. LPS-treated group. The data are the mean ± SEM (N = 5–6 in each group). *P < 0.05 vs. V+V, ** P < 0.05 vs. LPS+V, †P < 0.05 vs. LPS+LPS, ‡P < 0.05 vs. V+LPS. BALF, bronchoalveolar lavage fluid; PB, peripheral blood; V, vehicle; WBC, white blood cell.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig3: Bronchoalveolar lavage fluid and peripheral blood white blood cell and neutrophil counts. (a, b, d, e) Gray bars denote P7 and black bars P14. (a) The total BALF WBC counts were higher in the i.p. LPS-treated groups than in the i.p. vehicle-treated groups on P7 and P14. (b) The V+LPS group had higher BALF neutrophil counts than in other groups on P7 and in the V+V group on P14. (c) Representative light microscopic images of the cytospin BALF samples on P7. A marked increase in the number of neutrophils in the BALF cytospin sample from the V+LPS group is observed compared with the other groups. The arrows indicate neutrophils. Diff-Quik (Sysmex, Kobe, Japan) stained. Original magnification ×400. The bar indicates 50 μm. (d, e) The total PB WBC and neutrophil counts were highest in the V+LPS group on P7. On P14, the total WBC and neutrophil counts in PB were reduced compared with P7, and the V+V group had the highest number of total WBC and neutrophil counts in PB. Group abbreviation: V+V, i.a. vehicle followed by i.p. vehicle-treated group; LPS+V, i.a. LPS followed by i.p. vehicle-treated group; V+LPS, i.a. vehicle followed by i.p. LPS-treated group; LPS+LPS, i.a. LPS followed by i.p. LPS-treated group. The data are the mean ± SEM (N = 5–6 in each group). *P < 0.05 vs. V+V, ** P < 0.05 vs. LPS+V, †P < 0.05 vs. LPS+LPS, ‡P < 0.05 vs. V+LPS. BALF, bronchoalveolar lavage fluid; PB, peripheral blood; V, vehicle; WBC, white blood cell.
Mentions: Postnatal i.p. LPS significantly increased the total white blood cell (WBC) counts in the bronchoalveolar lavage fluid (BALF) at P7 and P14 (Figure 3a). The total BALF neutrophil counts on P7 were significantly higher in the V+LPS group compared with the other groups (Figure 3b,c). The total BALF neutrophil counts were markedly decreased in all groups on P14 compared with those at P7. However, the V+LPS group had a significantly higher number of BALF neutrophils than the V+V group. Similar findings were observed with the total WBC or neutrophil counts in peripheral blood (PB) on P7 (Figure 3d,e). Notably, the V+V group had the highest total PB WBC and neutrophil counts on P14 among the groups.

Bottom Line: LPS significantly negated the detrimental effects of postnatal i.p.Preceding exposure to i.a.LPS protects the lungs against BPD triggered by postnatal systemic inflammation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Seoul National University College of Medicine, Seoul, Republic of Korea.

ABSTRACT

Background: Prenatal or postnatal systemic inflammation can contribute to the development of bronchopulmonary dysplasia (BPD). We investigated whether prenatal intra-amniotic (i.a.) inflammation or early postnatal systemic inflammation can induce BPD in a rat model.

Methods: One microgram of lipopolysaccharide (LPS) or vehicle was injected into the amniotic sacs 2 d before delivery (E20). After birth, 0.25 mg/kg of LPS or vehicle was injected into the peritoneum of pups on postnatal day (P)1, P3, and P5. On P7 and P14, peripheral blood (PB), bronchoalveolar lavage fluid (BALF), and lung tissue were obtained and analyzed.

Results: Postnatal i.p. injections of LPS significantly increased neutrophil counts in PB and BALF on P7 and P14. Similarly, proinflammatory cytokine and angiogenic factor transcript levels were increased in the lung by i.p. LPS on P7. Alveolar and pulmonary vascular development was markedly disrupted by i.p. LPS on P14. However, pretreatment with i.a. LPS significantly negated the detrimental effects of postnatal i.p. LPS on PB and BALF neutrophil counts and on lung proinflammatory cytokine expression and histopathological changes.

Conclusion: Exposure to early postnatal systemic LPS induces BPD, an arrest in alveolarization, in neonatal rats. Preceding exposure to i.a. LPS protects the lungs against BPD triggered by postnatal systemic inflammation.

No MeSH data available.


Related in: MedlinePlus