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A novel and highly sensitive nanocatalytic surface plasmon resonance-scattering analytical platform for detection of trace Pb ions.

Ye L, Wen G, Ouyang H, Liu Q, Liang A, Jiang Z - Sci Rep (2016)

Bottom Line: The DNAzyme strand hybridized with the substrate strand to form double-stranded DNA (dsDNA) which couldn't protect AuNPc to aggregate to AuNPc aggregations, having strong RRS effect.The increased RRS intensity responds linearly with Pb(2+) concentration over 16.7-666.7 nmol/L.The SERS intensity responded linearly with the concentration of Pb(2+) over 50-500 nmol/L.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Normal University, Guilin 541004, China.

ABSTRACT
Gold nanoparticles (AuNP) have catalysis on the reaction of HAuCl4-H2O2. The produced AuNP have strong resonance Rayleigh scattering (RRS) effect and surface-enhanced resonance Raman scattering (SERS) effect when Victoria blue B (VBB) and rhodamine S (RhS) were used as probes. The increased RRS/SERS intensity respond linearly with the concentration of gold nanoparticles (AuNPB) which synthesized by NaBH4 over 0.038-76 ng/mL, 19-285 ng/mL, 3.8-456 ng/mL respectively. Four kinds of tested nanoparticles have catalysis on the HAuCl4-H2O2 particles reaction. Thus, a novel nanocatalysis surface plasmon resonance-scattering (SPR-S) analytical platform was developed for AuNP. The DNAzyme strand hybridized with the substrate strand to form double-stranded DNA (dsDNA) which couldn't protect AuNPc to aggregate to AuNPc aggregations, having strong RRS effect. Upon addition of Pb(2+), dsDNA could be cracked by Pb(2+) to produce single-stranded DNA (ssDNA) that adsorbed on the AuNPc surface to form AuNPc-ssDNA conjugates. The conjugates have strong catalysis on HAuCl4-H2O2 reaction. With increased Pb(2+) concentration, the concentration of AuNPc-ssDNA increased and lead to the catalytic activity stronger. The increased RRS intensity responds linearly with Pb(2+) concentration over 16.7-666.7 nmol/L. The SERS intensity responded linearly with the concentration of Pb(2+) over 50-500 nmol/L.

No MeSH data available.


RRS spectrum of AuNPc-HAuCl4-H2O2 system.(a) 4.48 μmol/L HAuCl4+0.67 mmol/L HCl+3.33 mmol/L H2O2 (b) a+1.9 ng/mL AuNPc; (c) a+3.8 ng/mL AuNPc; (d) a+7.6 ng/mL AuNPc; (e) a+19 ng/mL AuNPc; (f) a+34.2 ng/mL AuNPc; (g) a+47.ng/mL AuNPc; (h) a+85.5 ng/mL AuNPc; (i) a+133 ng/mL AuNPc; (j) a+161.5 ng/mL AuNPc.
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f1: RRS spectrum of AuNPc-HAuCl4-H2O2 system.(a) 4.48 μmol/L HAuCl4+0.67 mmol/L HCl+3.33 mmol/L H2O2 (b) a+1.9 ng/mL AuNPc; (c) a+3.8 ng/mL AuNPc; (d) a+7.6 ng/mL AuNPc; (e) a+19 ng/mL AuNPc; (f) a+34.2 ng/mL AuNPc; (g) a+47.ng/mL AuNPc; (h) a+85.5 ng/mL AuNPc; (i) a+133 ng/mL AuNPc; (j) a+161.5 ng/mL AuNPc.

Mentions: The RRS signals of small particle size gold, silver, platinum and palladium nanosol are very weak. Different concentrations of AgNO3 were added to preparation of AuNR1, AuNR2 and AuNR3, with the diameter of 32 nm, 37 nm and 43 nm respectively that RRS values gradually reduced (Fig. S1). With the increase of AuNR concentration, the RRS peak linear increased at 370 nm (Fig. S1D). Nanoparticles can catalytic hydrogen peroxide reduction of HAuCl4 under the condition of 0.67 mmol/L HCl, and with the increase of nanogold solution concentration, the RRS intensity of system linear increase at 370 nm (Fig. 1, Fig. S2). The catalytic activity of AuNPB was better than that of AuNPc because the particle size of AuNPB was smaller than AuNPc, which lead to the surface energy more larger and the surface of AuNPB nanoparticles can absorb more HAuCl4. Different particle size of AuNR was added as catalyst, with the AuNR concentration increased , the RRS peak linear increased at 370 nm (Figs S3–S5). When AgNPs, PdNPs, PtNPs nanosol solution was used as catalyst, with the increase of nanoparticles concentration, the RRS peak linear increased at 370 nm (Fig. S6). It can be used to quantitative of HAuCl4 and H2O2 through this catalytic system, with the increase of HAuCl4 concentration, the RRS peak of system linear increased and color from colorless gradually became red (Fig. S7), with the increase of H2O2 concentration, the RRS peak of system linear increased (Fig. S8). When AuNPc-ssDNA solution was used as catalyst, AuNPc modified by aptamer catalytic activity is stronger than AuNPc solution, with the increase of AuNPc-ssDNA concentration, the RRS peak linear increased at 370 nm (Fig. S9).


A novel and highly sensitive nanocatalytic surface plasmon resonance-scattering analytical platform for detection of trace Pb ions.

Ye L, Wen G, Ouyang H, Liu Q, Liang A, Jiang Z - Sci Rep (2016)

RRS spectrum of AuNPc-HAuCl4-H2O2 system.(a) 4.48 μmol/L HAuCl4+0.67 mmol/L HCl+3.33 mmol/L H2O2 (b) a+1.9 ng/mL AuNPc; (c) a+3.8 ng/mL AuNPc; (d) a+7.6 ng/mL AuNPc; (e) a+19 ng/mL AuNPc; (f) a+34.2 ng/mL AuNPc; (g) a+47.ng/mL AuNPc; (h) a+85.5 ng/mL AuNPc; (i) a+133 ng/mL AuNPc; (j) a+161.5 ng/mL AuNPc.
© Copyright Policy - open-access
Related In: Results  -  Collection

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f1: RRS spectrum of AuNPc-HAuCl4-H2O2 system.(a) 4.48 μmol/L HAuCl4+0.67 mmol/L HCl+3.33 mmol/L H2O2 (b) a+1.9 ng/mL AuNPc; (c) a+3.8 ng/mL AuNPc; (d) a+7.6 ng/mL AuNPc; (e) a+19 ng/mL AuNPc; (f) a+34.2 ng/mL AuNPc; (g) a+47.ng/mL AuNPc; (h) a+85.5 ng/mL AuNPc; (i) a+133 ng/mL AuNPc; (j) a+161.5 ng/mL AuNPc.
Mentions: The RRS signals of small particle size gold, silver, platinum and palladium nanosol are very weak. Different concentrations of AgNO3 were added to preparation of AuNR1, AuNR2 and AuNR3, with the diameter of 32 nm, 37 nm and 43 nm respectively that RRS values gradually reduced (Fig. S1). With the increase of AuNR concentration, the RRS peak linear increased at 370 nm (Fig. S1D). Nanoparticles can catalytic hydrogen peroxide reduction of HAuCl4 under the condition of 0.67 mmol/L HCl, and with the increase of nanogold solution concentration, the RRS intensity of system linear increase at 370 nm (Fig. 1, Fig. S2). The catalytic activity of AuNPB was better than that of AuNPc because the particle size of AuNPB was smaller than AuNPc, which lead to the surface energy more larger and the surface of AuNPB nanoparticles can absorb more HAuCl4. Different particle size of AuNR was added as catalyst, with the AuNR concentration increased , the RRS peak linear increased at 370 nm (Figs S3–S5). When AgNPs, PdNPs, PtNPs nanosol solution was used as catalyst, with the increase of nanoparticles concentration, the RRS peak linear increased at 370 nm (Fig. S6). It can be used to quantitative of HAuCl4 and H2O2 through this catalytic system, with the increase of HAuCl4 concentration, the RRS peak of system linear increased and color from colorless gradually became red (Fig. S7), with the increase of H2O2 concentration, the RRS peak of system linear increased (Fig. S8). When AuNPc-ssDNA solution was used as catalyst, AuNPc modified by aptamer catalytic activity is stronger than AuNPc solution, with the increase of AuNPc-ssDNA concentration, the RRS peak linear increased at 370 nm (Fig. S9).

Bottom Line: The DNAzyme strand hybridized with the substrate strand to form double-stranded DNA (dsDNA) which couldn't protect AuNPc to aggregate to AuNPc aggregations, having strong RRS effect.The increased RRS intensity responds linearly with Pb(2+) concentration over 16.7-666.7 nmol/L.The SERS intensity responded linearly with the concentration of Pb(2+) over 50-500 nmol/L.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of Ministry Education, Guangxi Normal University, Guilin 541004, China.

ABSTRACT
Gold nanoparticles (AuNP) have catalysis on the reaction of HAuCl4-H2O2. The produced AuNP have strong resonance Rayleigh scattering (RRS) effect and surface-enhanced resonance Raman scattering (SERS) effect when Victoria blue B (VBB) and rhodamine S (RhS) were used as probes. The increased RRS/SERS intensity respond linearly with the concentration of gold nanoparticles (AuNPB) which synthesized by NaBH4 over 0.038-76 ng/mL, 19-285 ng/mL, 3.8-456 ng/mL respectively. Four kinds of tested nanoparticles have catalysis on the HAuCl4-H2O2 particles reaction. Thus, a novel nanocatalysis surface plasmon resonance-scattering (SPR-S) analytical platform was developed for AuNP. The DNAzyme strand hybridized with the substrate strand to form double-stranded DNA (dsDNA) which couldn't protect AuNPc to aggregate to AuNPc aggregations, having strong RRS effect. Upon addition of Pb(2+), dsDNA could be cracked by Pb(2+) to produce single-stranded DNA (ssDNA) that adsorbed on the AuNPc surface to form AuNPc-ssDNA conjugates. The conjugates have strong catalysis on HAuCl4-H2O2 reaction. With increased Pb(2+) concentration, the concentration of AuNPc-ssDNA increased and lead to the catalytic activity stronger. The increased RRS intensity responds linearly with Pb(2+) concentration over 16.7-666.7 nmol/L. The SERS intensity responded linearly with the concentration of Pb(2+) over 50-500 nmol/L.

No MeSH data available.