Limits...
A novel open-porous magnesium scaffold with controllable microstructures and properties for bone regeneration.

Cheng MQ, Wahafu T, Jiang GF, Liu W, Qiao YQ, Peng XC, Cheng T, Zhang XL, He G, Liu XY - Sci Rep (2016)

Bottom Line: The porosity and pore size can be easily, precisely and individually controlled, as well as the mechanical properties also can be regulated to be within the range of human cancellous bone by changing the orientation of pores without sacrifice the requisite porous structures.In vitro cell tests indicate that the scaffolds have good cytocompatibility and osteoblastic differentiation properties.In conclusion, a new method is introduced to develop an open-porous magnesium scaffold with controllable microstructures and mechanical properties, which has great potential clinical application for bone reconstruction in the future.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China.

ABSTRACT
The traditional production methods of porous magnesium scaffolds are difficult to accurately control the pore morphologies and simultaneously obtain appropriate mechanical properties. In this work, two open-porous magnesium scaffolds with different pore size but in the nearly same porosity are successfully fabricated with high-purity Mg ingots through the titanium wire space holder (TWSH) method. The porosity and pore size can be easily, precisely and individually controlled, as well as the mechanical properties also can be regulated to be within the range of human cancellous bone by changing the orientation of pores without sacrifice the requisite porous structures. In vitro cell tests indicate that the scaffolds have good cytocompatibility and osteoblastic differentiation properties. In vivo findings demonstrate that both scaffolds exhibit acceptable inflammatory responses and can be almost fully degraded and replaced by newly formed bone. More importantly, under the same porosity, the scaffolds with larger pore size can promote early vascularization and up-regulate collagen type 1 and OPN expression, leading to higher bone mass and more mature bone formation. In conclusion, a new method is introduced to develop an open-porous magnesium scaffold with controllable microstructures and mechanical properties, which has great potential clinical application for bone reconstruction in the future.

No MeSH data available.


Related in: MedlinePlus

(a) H&E staining of subcutaneous and muscular tissue in contact with 250-PMg and 400-PMg scaffold, the pentagrams represent the fibrous tissues formed around the scaffolds; (b) Percentage changes in serum magnesium levels before and after implantation; (c) Representative sections from important internal organs of the rabbits after implantation with 250-PMg and 400-PMg scaffold for 16 weeks.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4829853&req=5

f5: (a) H&E staining of subcutaneous and muscular tissue in contact with 250-PMg and 400-PMg scaffold, the pentagrams represent the fibrous tissues formed around the scaffolds; (b) Percentage changes in serum magnesium levels before and after implantation; (c) Representative sections from important internal organs of the rabbits after implantation with 250-PMg and 400-PMg scaffold for 16 weeks.

Mentions: Figure 5a shows the histological images of the localized sites of implants harvested from subcutaneous and intramuscular tissues surrounding 250-PMg and 400-PMg scaffolds after 8 weeks. Although fibrous tissues containing a mild population of agglomerated cells and macrophages were observed surrounding the two scaffolds, the parameters for inflammation examined for all implants in this study did not exceed the moderate level.


A novel open-porous magnesium scaffold with controllable microstructures and properties for bone regeneration.

Cheng MQ, Wahafu T, Jiang GF, Liu W, Qiao YQ, Peng XC, Cheng T, Zhang XL, He G, Liu XY - Sci Rep (2016)

(a) H&E staining of subcutaneous and muscular tissue in contact with 250-PMg and 400-PMg scaffold, the pentagrams represent the fibrous tissues formed around the scaffolds; (b) Percentage changes in serum magnesium levels before and after implantation; (c) Representative sections from important internal organs of the rabbits after implantation with 250-PMg and 400-PMg scaffold for 16 weeks.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829853&req=5

f5: (a) H&E staining of subcutaneous and muscular tissue in contact with 250-PMg and 400-PMg scaffold, the pentagrams represent the fibrous tissues formed around the scaffolds; (b) Percentage changes in serum magnesium levels before and after implantation; (c) Representative sections from important internal organs of the rabbits after implantation with 250-PMg and 400-PMg scaffold for 16 weeks.
Mentions: Figure 5a shows the histological images of the localized sites of implants harvested from subcutaneous and intramuscular tissues surrounding 250-PMg and 400-PMg scaffolds after 8 weeks. Although fibrous tissues containing a mild population of agglomerated cells and macrophages were observed surrounding the two scaffolds, the parameters for inflammation examined for all implants in this study did not exceed the moderate level.

Bottom Line: The porosity and pore size can be easily, precisely and individually controlled, as well as the mechanical properties also can be regulated to be within the range of human cancellous bone by changing the orientation of pores without sacrifice the requisite porous structures.In vitro cell tests indicate that the scaffolds have good cytocompatibility and osteoblastic differentiation properties.In conclusion, a new method is introduced to develop an open-porous magnesium scaffold with controllable microstructures and mechanical properties, which has great potential clinical application for bone reconstruction in the future.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China.

ABSTRACT
The traditional production methods of porous magnesium scaffolds are difficult to accurately control the pore morphologies and simultaneously obtain appropriate mechanical properties. In this work, two open-porous magnesium scaffolds with different pore size but in the nearly same porosity are successfully fabricated with high-purity Mg ingots through the titanium wire space holder (TWSH) method. The porosity and pore size can be easily, precisely and individually controlled, as well as the mechanical properties also can be regulated to be within the range of human cancellous bone by changing the orientation of pores without sacrifice the requisite porous structures. In vitro cell tests indicate that the scaffolds have good cytocompatibility and osteoblastic differentiation properties. In vivo findings demonstrate that both scaffolds exhibit acceptable inflammatory responses and can be almost fully degraded and replaced by newly formed bone. More importantly, under the same porosity, the scaffolds with larger pore size can promote early vascularization and up-regulate collagen type 1 and OPN expression, leading to higher bone mass and more mature bone formation. In conclusion, a new method is introduced to develop an open-porous magnesium scaffold with controllable microstructures and mechanical properties, which has great potential clinical application for bone reconstruction in the future.

No MeSH data available.


Related in: MedlinePlus