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Evaluation of in vitro and in vivo biocompatibility of a myo-inositol hexakisphosphate gelated polyaniline hydrogel in a rat model.

Sun KH, Liu Z, Liu C, Yu T, Shang T, Huang C, Zhou M, Liu C, Ran F, Li Y, Shi Y, Pan L - Sci Rep (2016)

Bottom Line: In this study, we investigated the biocompatibility of a myo-inositol hexakisphosphate gelated polyaniline hydrogel using in vitro and in vivo experiments in a rat model.The polyaniline hydrogel was used to coat a polycaprolactone scaffold and was cultured with rat endothelial progenitor cells differentiated from rat adipose-derived stem cells.In implant studies, the polyaniline hydrogel sample induced milder inflammatory responses than did the control at the same time points.

View Article: PubMed Central - PubMed

Affiliation: Department of Vascular Surgery, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

ABSTRACT
Recent advances in understanding the interaction between electricity and cells/biomolecules have generated great interest in developing biocompatible electrically conductive materials. In this study, we investigated the biocompatibility of a myo-inositol hexakisphosphate gelated polyaniline hydrogel using in vitro and in vivo experiments in a rat model. The polyaniline hydrogel was used to coat a polycaprolactone scaffold and was cultured with rat endothelial progenitor cells differentiated from rat adipose-derived stem cells. Compared with the control sample on a pristine polycaprolactone scaffold, the treated polyaniline hydrogel had the same non-poisonous/cytotoxicity grade, enhanced cell adhesion, and a higher cell proliferation/growth rate. In implant studies, the polyaniline hydrogel sample induced milder inflammatory responses than did the control at the same time points. Combining the advantages of a biocompatible hydrogel and an organic conductor, the inositol phosphate-gelated polyaniline hydrogel could be used in bioelectronics applications such as biosensors, neural probes, cell stimulators, medical electrodes, tissue engineering, and electro-controlled drug delivery.

No MeSH data available.


Related in: MedlinePlus

Average diameter of cells after seeding and culturing on the PCPS and BPS surfaces.(A) PCPS (1 day), (B) PCPS (3 days), (C) PCPS (7 days), (D) BPS (1 day), (E) BPS (3 days), and (F) BPS (7 days).
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f6: Average diameter of cells after seeding and culturing on the PCPS and BPS surfaces.(A) PCPS (1 day), (B) PCPS (3 days), (C) PCPS (7 days), (D) BPS (1 day), (E) BPS (3 days), and (F) BPS (7 days).

Mentions: SEM images of rEPCs grown on the PCPS and BPS surfaces are shown in Fig. 5. At each time point, the PCPS group exhibited better cell adhesion, development and proliferation (Fig. 5A–C) than the BPS group (Fig. 5D–F). The cells grown on PCPS (Fig. 5A–C) were plump and had a much larger cell diameter compared with that of the BPS group (Fig. 5D–F). The average cell diameters of the PCPS and BPS groups are presented in Fig. 6. The average cell diameters of the PCPS and BPS groups were 21.52 μm vs. 12.35 μm (1 day), 21.37 μm vs. 12.45 μm (3 days) and 25.16 μm vs. 11.85 μm (7 days), respectively. The quantity of cells in unit area of the PCPS and BPS group are presented in Fig. 7. The quantity of cells in unit area of the PCPS and BPS group were 718 cells/mm2 vs. 513 cells/mm2 (1 day), 1428 cells/mm2 vs. 1300 cells/mm2 (3 day) and 1505 cells/mm2 vs. 1411 cells/mm2 (7 day), respectively.


Evaluation of in vitro and in vivo biocompatibility of a myo-inositol hexakisphosphate gelated polyaniline hydrogel in a rat model.

Sun KH, Liu Z, Liu C, Yu T, Shang T, Huang C, Zhou M, Liu C, Ran F, Li Y, Shi Y, Pan L - Sci Rep (2016)

Average diameter of cells after seeding and culturing on the PCPS and BPS surfaces.(A) PCPS (1 day), (B) PCPS (3 days), (C) PCPS (7 days), (D) BPS (1 day), (E) BPS (3 days), and (F) BPS (7 days).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829851&req=5

f6: Average diameter of cells after seeding and culturing on the PCPS and BPS surfaces.(A) PCPS (1 day), (B) PCPS (3 days), (C) PCPS (7 days), (D) BPS (1 day), (E) BPS (3 days), and (F) BPS (7 days).
Mentions: SEM images of rEPCs grown on the PCPS and BPS surfaces are shown in Fig. 5. At each time point, the PCPS group exhibited better cell adhesion, development and proliferation (Fig. 5A–C) than the BPS group (Fig. 5D–F). The cells grown on PCPS (Fig. 5A–C) were plump and had a much larger cell diameter compared with that of the BPS group (Fig. 5D–F). The average cell diameters of the PCPS and BPS groups are presented in Fig. 6. The average cell diameters of the PCPS and BPS groups were 21.52 μm vs. 12.35 μm (1 day), 21.37 μm vs. 12.45 μm (3 days) and 25.16 μm vs. 11.85 μm (7 days), respectively. The quantity of cells in unit area of the PCPS and BPS group are presented in Fig. 7. The quantity of cells in unit area of the PCPS and BPS group were 718 cells/mm2 vs. 513 cells/mm2 (1 day), 1428 cells/mm2 vs. 1300 cells/mm2 (3 day) and 1505 cells/mm2 vs. 1411 cells/mm2 (7 day), respectively.

Bottom Line: In this study, we investigated the biocompatibility of a myo-inositol hexakisphosphate gelated polyaniline hydrogel using in vitro and in vivo experiments in a rat model.The polyaniline hydrogel was used to coat a polycaprolactone scaffold and was cultured with rat endothelial progenitor cells differentiated from rat adipose-derived stem cells.In implant studies, the polyaniline hydrogel sample induced milder inflammatory responses than did the control at the same time points.

View Article: PubMed Central - PubMed

Affiliation: Department of Vascular Surgery, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

ABSTRACT
Recent advances in understanding the interaction between electricity and cells/biomolecules have generated great interest in developing biocompatible electrically conductive materials. In this study, we investigated the biocompatibility of a myo-inositol hexakisphosphate gelated polyaniline hydrogel using in vitro and in vivo experiments in a rat model. The polyaniline hydrogel was used to coat a polycaprolactone scaffold and was cultured with rat endothelial progenitor cells differentiated from rat adipose-derived stem cells. Compared with the control sample on a pristine polycaprolactone scaffold, the treated polyaniline hydrogel had the same non-poisonous/cytotoxicity grade, enhanced cell adhesion, and a higher cell proliferation/growth rate. In implant studies, the polyaniline hydrogel sample induced milder inflammatory responses than did the control at the same time points. Combining the advantages of a biocompatible hydrogel and an organic conductor, the inositol phosphate-gelated polyaniline hydrogel could be used in bioelectronics applications such as biosensors, neural probes, cell stimulators, medical electrodes, tissue engineering, and electro-controlled drug delivery.

No MeSH data available.


Related in: MedlinePlus