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Evaluation of in vitro and in vivo biocompatibility of a myo-inositol hexakisphosphate gelated polyaniline hydrogel in a rat model.

Sun KH, Liu Z, Liu C, Yu T, Shang T, Huang C, Zhou M, Liu C, Ran F, Li Y, Shi Y, Pan L - Sci Rep (2016)

Bottom Line: In this study, we investigated the biocompatibility of a myo-inositol hexakisphosphate gelated polyaniline hydrogel using in vitro and in vivo experiments in a rat model.The polyaniline hydrogel was used to coat a polycaprolactone scaffold and was cultured with rat endothelial progenitor cells differentiated from rat adipose-derived stem cells.In implant studies, the polyaniline hydrogel sample induced milder inflammatory responses than did the control at the same time points.

View Article: PubMed Central - PubMed

Affiliation: Department of Vascular Surgery, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

ABSTRACT
Recent advances in understanding the interaction between electricity and cells/biomolecules have generated great interest in developing biocompatible electrically conductive materials. In this study, we investigated the biocompatibility of a myo-inositol hexakisphosphate gelated polyaniline hydrogel using in vitro and in vivo experiments in a rat model. The polyaniline hydrogel was used to coat a polycaprolactone scaffold and was cultured with rat endothelial progenitor cells differentiated from rat adipose-derived stem cells. Compared with the control sample on a pristine polycaprolactone scaffold, the treated polyaniline hydrogel had the same non-poisonous/cytotoxicity grade, enhanced cell adhesion, and a higher cell proliferation/growth rate. In implant studies, the polyaniline hydrogel sample induced milder inflammatory responses than did the control at the same time points. Combining the advantages of a biocompatible hydrogel and an organic conductor, the inositol phosphate-gelated polyaniline hydrogel could be used in bioelectronics applications such as biosensors, neural probes, cell stimulators, medical electrodes, tissue engineering, and electro-controlled drug delivery.

No MeSH data available.


Related in: MedlinePlus

Immunofluorescence images of rEPCs seeded on the PCPS and BPS surfaces and then cultured for 1, 3 and 7 days (VWF positive staining) (×200).(A) PCPS (1 day), (B) PCPS (3 days), (C) PCPS (7 days), (D) BPS (1 day), (E) BPS (3 days), and (F) BPS (7 days).
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f3: Immunofluorescence images of rEPCs seeded on the PCPS and BPS surfaces and then cultured for 1, 3 and 7 days (VWF positive staining) (×200).(A) PCPS (1 day), (B) PCPS (3 days), (C) PCPS (7 days), (D) BPS (1 day), (E) BPS (3 days), and (F) BPS (7 days).

Mentions: Immunofluorescence microscopy and scanning electron microscopy (SEM) were used to investigate the morphology of the rEPCs that grew on the PCPS and BPS surfaces. The immunofluorescence images are presented in Fig. 3. The quantity of cells on the surfaces of the scaffolds increased with the duration of cultivation in the same group. Notably, compared with the BPS group (Fig. 3D–F), the PCPS group (Fig. 3A–C) exhibited greatly enhanced adhesion and proliferation. The area specific cell density (defined as the area covered with cells relative to the surface area of the substrate) values were 25–28% vs. 5–6% on day 1, 45–51% vs. 26–29% on day 3, and 53–60% vs. 42–47% on day 7 for the PCPS groups vs. the BPS group (Fig. 4).


Evaluation of in vitro and in vivo biocompatibility of a myo-inositol hexakisphosphate gelated polyaniline hydrogel in a rat model.

Sun KH, Liu Z, Liu C, Yu T, Shang T, Huang C, Zhou M, Liu C, Ran F, Li Y, Shi Y, Pan L - Sci Rep (2016)

Immunofluorescence images of rEPCs seeded on the PCPS and BPS surfaces and then cultured for 1, 3 and 7 days (VWF positive staining) (×200).(A) PCPS (1 day), (B) PCPS (3 days), (C) PCPS (7 days), (D) BPS (1 day), (E) BPS (3 days), and (F) BPS (7 days).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829851&req=5

f3: Immunofluorescence images of rEPCs seeded on the PCPS and BPS surfaces and then cultured for 1, 3 and 7 days (VWF positive staining) (×200).(A) PCPS (1 day), (B) PCPS (3 days), (C) PCPS (7 days), (D) BPS (1 day), (E) BPS (3 days), and (F) BPS (7 days).
Mentions: Immunofluorescence microscopy and scanning electron microscopy (SEM) were used to investigate the morphology of the rEPCs that grew on the PCPS and BPS surfaces. The immunofluorescence images are presented in Fig. 3. The quantity of cells on the surfaces of the scaffolds increased with the duration of cultivation in the same group. Notably, compared with the BPS group (Fig. 3D–F), the PCPS group (Fig. 3A–C) exhibited greatly enhanced adhesion and proliferation. The area specific cell density (defined as the area covered with cells relative to the surface area of the substrate) values were 25–28% vs. 5–6% on day 1, 45–51% vs. 26–29% on day 3, and 53–60% vs. 42–47% on day 7 for the PCPS groups vs. the BPS group (Fig. 4).

Bottom Line: In this study, we investigated the biocompatibility of a myo-inositol hexakisphosphate gelated polyaniline hydrogel using in vitro and in vivo experiments in a rat model.The polyaniline hydrogel was used to coat a polycaprolactone scaffold and was cultured with rat endothelial progenitor cells differentiated from rat adipose-derived stem cells.In implant studies, the polyaniline hydrogel sample induced milder inflammatory responses than did the control at the same time points.

View Article: PubMed Central - PubMed

Affiliation: Department of Vascular Surgery, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

ABSTRACT
Recent advances in understanding the interaction between electricity and cells/biomolecules have generated great interest in developing biocompatible electrically conductive materials. In this study, we investigated the biocompatibility of a myo-inositol hexakisphosphate gelated polyaniline hydrogel using in vitro and in vivo experiments in a rat model. The polyaniline hydrogel was used to coat a polycaprolactone scaffold and was cultured with rat endothelial progenitor cells differentiated from rat adipose-derived stem cells. Compared with the control sample on a pristine polycaprolactone scaffold, the treated polyaniline hydrogel had the same non-poisonous/cytotoxicity grade, enhanced cell adhesion, and a higher cell proliferation/growth rate. In implant studies, the polyaniline hydrogel sample induced milder inflammatory responses than did the control at the same time points. Combining the advantages of a biocompatible hydrogel and an organic conductor, the inositol phosphate-gelated polyaniline hydrogel could be used in bioelectronics applications such as biosensors, neural probes, cell stimulators, medical electrodes, tissue engineering, and electro-controlled drug delivery.

No MeSH data available.


Related in: MedlinePlus