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Streptomyces malaysiense sp. nov.: A novel Malaysian mangrove soil actinobacterium with antioxidative activity and cytotoxic potential against human cancer cell lines.

Ser HL, Palanisamy UD, Yin WF, Chan KG, Goh BH, Lee LH - Sci Rep (2016)

Bottom Line: Phylogenetically, highest similarity was to Streptomyces misionensis NBRC 13063(T) (99.6%) along with two other strains (>98.9% sequence similarities).The DNA-DNA relatedness between MUSC 136(T) and these type strains ranged from 22.7 ± 0.5% to 46.5 ± 0.2%.It was also found to possess high cytotoxic effect against HCT-116 cells, which probably mediated through altering p53 protein and intracellular glutathione levels.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Monash University Malaysia, 47500 Bandar Sunway, Malaysia.

ABSTRACT
Actinobacteria from the unique intertidal ecosystem of the mangroves are known to produce novel, bioactive secondary metabolites. A novel strain known as MUSC 136(T) (=DSM 100712(T) = MCCC 1K01246(T)) which was isolated from Malaysian mangrove forest soil has proven to be no exception. Assessed by a polyphasic approach, its taxonomy showed a range of phylogenetic and chemotaxonomic properties consistent with the genus of Streptomyces. Phylogenetically, highest similarity was to Streptomyces misionensis NBRC 13063(T) (99.6%) along with two other strains (>98.9% sequence similarities). The DNA-DNA relatedness between MUSC 136(T) and these type strains ranged from 22.7 ± 0.5% to 46.5 ± 0.2%. Overall, polyphasic approach studies indicated this strain represents a novel species, for which the name Streptomyces malaysiense sp. nov. is proposed. The potential bioactivities of this strain were explored by means of antioxidant and cytotoxic assays. Intriguingly, MUSC 136(T) exhibited strong antioxidative activities as evaluated by a panel of antioxidant assays. It was also found to possess high cytotoxic effect against HCT-116 cells, which probably mediated through altering p53 protein and intracellular glutathione levels. Chemical analysis of the extract using GC-MS further affirms that the strain produces chemopreventive related metabolites.

No MeSH data available.


Related in: MedlinePlus

Cytotoxic activity of MUSC 136T extract against human cancer cell lines.The measurement of cell viability was done using MTT assay. The graphs show cytotoxic effect of MUSC 136T extract against (a) HCT-116, (b) Ca Ski, (c) A549, (d) HT-29. All data are expressed as mean ± standard deviation (n = 4) and analyzed using one-way analysis of variance (ANOVA). A difference was considered statistically significant when p ≤ 0.05. (e) Morphological studies show that after treatment with the extract, cells shrunk to smaller rounder cells and detached from the surface.
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f3: Cytotoxic activity of MUSC 136T extract against human cancer cell lines.The measurement of cell viability was done using MTT assay. The graphs show cytotoxic effect of MUSC 136T extract against (a) HCT-116, (b) Ca Ski, (c) A549, (d) HT-29. All data are expressed as mean ± standard deviation (n = 4) and analyzed using one-way analysis of variance (ANOVA). A difference was considered statistically significant when p ≤ 0.05. (e) Morphological studies show that after treatment with the extract, cells shrunk to smaller rounder cells and detached from the surface.

Mentions: The cytotoxic potential of MUSC 136T extract was tested against several human derived cancer cells (HCT-116, HT-29, Ca Ski and A549). The extract was found to be most toxic against HCT-116 with cell viability at 48.8 ± 4.1% when tested at 400 μg/mL (Fig. 3a). Additionally, a dose dependent effect was also observed when it was tested against HCT-116 cells. Second to HCT-116 cells, the cervical cancer cell line, Ca Ski showed decreased in cell viability to 55.6 ± 1.2% after treatment with MUSC 136T extract (Fig. 3b). On the other hand, lung cancer cell line, A549 was found to be least sensitive to the extract treatment with cell viability at 67.1 ± 0.6% (Fig. 3c). Interestingly, differing levels of activity were seen among the colon cancer cells (HCT-116, Fig. 3a and HT-29, Fig. 3d) with a higher cytotoxic effect against HCT-116 cells.


Streptomyces malaysiense sp. nov.: A novel Malaysian mangrove soil actinobacterium with antioxidative activity and cytotoxic potential against human cancer cell lines.

Ser HL, Palanisamy UD, Yin WF, Chan KG, Goh BH, Lee LH - Sci Rep (2016)

Cytotoxic activity of MUSC 136T extract against human cancer cell lines.The measurement of cell viability was done using MTT assay. The graphs show cytotoxic effect of MUSC 136T extract against (a) HCT-116, (b) Ca Ski, (c) A549, (d) HT-29. All data are expressed as mean ± standard deviation (n = 4) and analyzed using one-way analysis of variance (ANOVA). A difference was considered statistically significant when p ≤ 0.05. (e) Morphological studies show that after treatment with the extract, cells shrunk to smaller rounder cells and detached from the surface.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829849&req=5

f3: Cytotoxic activity of MUSC 136T extract against human cancer cell lines.The measurement of cell viability was done using MTT assay. The graphs show cytotoxic effect of MUSC 136T extract against (a) HCT-116, (b) Ca Ski, (c) A549, (d) HT-29. All data are expressed as mean ± standard deviation (n = 4) and analyzed using one-way analysis of variance (ANOVA). A difference was considered statistically significant when p ≤ 0.05. (e) Morphological studies show that after treatment with the extract, cells shrunk to smaller rounder cells and detached from the surface.
Mentions: The cytotoxic potential of MUSC 136T extract was tested against several human derived cancer cells (HCT-116, HT-29, Ca Ski and A549). The extract was found to be most toxic against HCT-116 with cell viability at 48.8 ± 4.1% when tested at 400 μg/mL (Fig. 3a). Additionally, a dose dependent effect was also observed when it was tested against HCT-116 cells. Second to HCT-116 cells, the cervical cancer cell line, Ca Ski showed decreased in cell viability to 55.6 ± 1.2% after treatment with MUSC 136T extract (Fig. 3b). On the other hand, lung cancer cell line, A549 was found to be least sensitive to the extract treatment with cell viability at 67.1 ± 0.6% (Fig. 3c). Interestingly, differing levels of activity were seen among the colon cancer cells (HCT-116, Fig. 3a and HT-29, Fig. 3d) with a higher cytotoxic effect against HCT-116 cells.

Bottom Line: Phylogenetically, highest similarity was to Streptomyces misionensis NBRC 13063(T) (99.6%) along with two other strains (>98.9% sequence similarities).The DNA-DNA relatedness between MUSC 136(T) and these type strains ranged from 22.7 ± 0.5% to 46.5 ± 0.2%.It was also found to possess high cytotoxic effect against HCT-116 cells, which probably mediated through altering p53 protein and intracellular glutathione levels.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Monash University Malaysia, 47500 Bandar Sunway, Malaysia.

ABSTRACT
Actinobacteria from the unique intertidal ecosystem of the mangroves are known to produce novel, bioactive secondary metabolites. A novel strain known as MUSC 136(T) (=DSM 100712(T) = MCCC 1K01246(T)) which was isolated from Malaysian mangrove forest soil has proven to be no exception. Assessed by a polyphasic approach, its taxonomy showed a range of phylogenetic and chemotaxonomic properties consistent with the genus of Streptomyces. Phylogenetically, highest similarity was to Streptomyces misionensis NBRC 13063(T) (99.6%) along with two other strains (>98.9% sequence similarities). The DNA-DNA relatedness between MUSC 136(T) and these type strains ranged from 22.7 ± 0.5% to 46.5 ± 0.2%. Overall, polyphasic approach studies indicated this strain represents a novel species, for which the name Streptomyces malaysiense sp. nov. is proposed. The potential bioactivities of this strain were explored by means of antioxidant and cytotoxic assays. Intriguingly, MUSC 136(T) exhibited strong antioxidative activities as evaluated by a panel of antioxidant assays. It was also found to possess high cytotoxic effect against HCT-116 cells, which probably mediated through altering p53 protein and intracellular glutathione levels. Chemical analysis of the extract using GC-MS further affirms that the strain produces chemopreventive related metabolites.

No MeSH data available.


Related in: MedlinePlus