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Streptomyces malaysiense sp. nov.: A novel Malaysian mangrove soil actinobacterium with antioxidative activity and cytotoxic potential against human cancer cell lines.

Ser HL, Palanisamy UD, Yin WF, Chan KG, Goh BH, Lee LH - Sci Rep (2016)

Bottom Line: Phylogenetically, highest similarity was to Streptomyces misionensis NBRC 13063(T) (99.6%) along with two other strains (>98.9% sequence similarities).The DNA-DNA relatedness between MUSC 136(T) and these type strains ranged from 22.7 ± 0.5% to 46.5 ± 0.2%.It was also found to possess high cytotoxic effect against HCT-116 cells, which probably mediated through altering p53 protein and intracellular glutathione levels.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Monash University Malaysia, 47500 Bandar Sunway, Malaysia.

ABSTRACT
Actinobacteria from the unique intertidal ecosystem of the mangroves are known to produce novel, bioactive secondary metabolites. A novel strain known as MUSC 136(T) (=DSM 100712(T) = MCCC 1K01246(T)) which was isolated from Malaysian mangrove forest soil has proven to be no exception. Assessed by a polyphasic approach, its taxonomy showed a range of phylogenetic and chemotaxonomic properties consistent with the genus of Streptomyces. Phylogenetically, highest similarity was to Streptomyces misionensis NBRC 13063(T) (99.6%) along with two other strains (>98.9% sequence similarities). The DNA-DNA relatedness between MUSC 136(T) and these type strains ranged from 22.7 ± 0.5% to 46.5 ± 0.2%. Overall, polyphasic approach studies indicated this strain represents a novel species, for which the name Streptomyces malaysiense sp. nov. is proposed. The potential bioactivities of this strain were explored by means of antioxidant and cytotoxic assays. Intriguingly, MUSC 136(T) exhibited strong antioxidative activities as evaluated by a panel of antioxidant assays. It was also found to possess high cytotoxic effect against HCT-116 cells, which probably mediated through altering p53 protein and intracellular glutathione levels. Chemical analysis of the extract using GC-MS further affirms that the strain produces chemopreventive related metabolites.

No MeSH data available.


Related in: MedlinePlus

Neighbour-joining phylogenetic tree based on almost complete 16S rRNA sequences (1489 nucleotides) showing the relationship between strain MUSC 136T and representatives of some other related taxa.Numbers at nodes indicate percentages of 1000 bootstrap re-samplings, only values above 50% are shown. Bar, 0.005 substitutions per site. Asterisks indicate that the corresponding nodes were also recovered using the maximum-likelihood tree-making algorithm. Streptacidiphilus albus NBRC 100918T was used as an outgroup.
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f2: Neighbour-joining phylogenetic tree based on almost complete 16S rRNA sequences (1489 nucleotides) showing the relationship between strain MUSC 136T and representatives of some other related taxa.Numbers at nodes indicate percentages of 1000 bootstrap re-samplings, only values above 50% are shown. Bar, 0.005 substitutions per site. Asterisks indicate that the corresponding nodes were also recovered using the maximum-likelihood tree-making algorithm. Streptacidiphilus albus NBRC 100918T was used as an outgroup.

Mentions: The nearly complete 16S rRNA gene sequence was obtained for strain MUSC 136T (1489 bp; GenBank/EMBL/DDBJ accession number KJ632663) and phylogenetic trees were reconstructed to determine the phylogenetic position of this strain (Fig. 2 & See Supplementary Fig. S1). Phylogenetic analysis revealed that strain MUSC 136T is closely related to Streptomyces phaeoluteichromatogenes NRRL 5799T (99.6% sequence similarity) and Streptomyces misionensis NBRC 13063T (99.6%), as they formed a distinct clade (Fig. 2). The 16S rRNA gene sequence analysis of strain MUSC 136T showed the highest similarity to that of Streptomyces misionensis NBRC 13063T (99.6%), followed by Streptomyces phaeoluteichromatogenes NRRL 5799T (99.6%) and Streptomyces rutgersensis NBRC 12819T (98.9%); sequences similarities of less than 98.9% were obtained with the type strains of other species of the genus Streptomyces. The DNA–DNA relatedness values between strain MUSC 136T and Streptomyces misionensis NBRC 13063T (46.5 ± 0.2%), S. phaeoluteichromatogenes DSM 41898T (44.5 ± 0.4%) and Streptomyces rutgersensis NBRC 12819T (22.7 ± 0.5%) were significantly below 70%, the threshold value for the delineation of bacterial species19.


Streptomyces malaysiense sp. nov.: A novel Malaysian mangrove soil actinobacterium with antioxidative activity and cytotoxic potential against human cancer cell lines.

Ser HL, Palanisamy UD, Yin WF, Chan KG, Goh BH, Lee LH - Sci Rep (2016)

Neighbour-joining phylogenetic tree based on almost complete 16S rRNA sequences (1489 nucleotides) showing the relationship between strain MUSC 136T and representatives of some other related taxa.Numbers at nodes indicate percentages of 1000 bootstrap re-samplings, only values above 50% are shown. Bar, 0.005 substitutions per site. Asterisks indicate that the corresponding nodes were also recovered using the maximum-likelihood tree-making algorithm. Streptacidiphilus albus NBRC 100918T was used as an outgroup.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829849&req=5

f2: Neighbour-joining phylogenetic tree based on almost complete 16S rRNA sequences (1489 nucleotides) showing the relationship between strain MUSC 136T and representatives of some other related taxa.Numbers at nodes indicate percentages of 1000 bootstrap re-samplings, only values above 50% are shown. Bar, 0.005 substitutions per site. Asterisks indicate that the corresponding nodes were also recovered using the maximum-likelihood tree-making algorithm. Streptacidiphilus albus NBRC 100918T was used as an outgroup.
Mentions: The nearly complete 16S rRNA gene sequence was obtained for strain MUSC 136T (1489 bp; GenBank/EMBL/DDBJ accession number KJ632663) and phylogenetic trees were reconstructed to determine the phylogenetic position of this strain (Fig. 2 & See Supplementary Fig. S1). Phylogenetic analysis revealed that strain MUSC 136T is closely related to Streptomyces phaeoluteichromatogenes NRRL 5799T (99.6% sequence similarity) and Streptomyces misionensis NBRC 13063T (99.6%), as they formed a distinct clade (Fig. 2). The 16S rRNA gene sequence analysis of strain MUSC 136T showed the highest similarity to that of Streptomyces misionensis NBRC 13063T (99.6%), followed by Streptomyces phaeoluteichromatogenes NRRL 5799T (99.6%) and Streptomyces rutgersensis NBRC 12819T (98.9%); sequences similarities of less than 98.9% were obtained with the type strains of other species of the genus Streptomyces. The DNA–DNA relatedness values between strain MUSC 136T and Streptomyces misionensis NBRC 13063T (46.5 ± 0.2%), S. phaeoluteichromatogenes DSM 41898T (44.5 ± 0.4%) and Streptomyces rutgersensis NBRC 12819T (22.7 ± 0.5%) were significantly below 70%, the threshold value for the delineation of bacterial species19.

Bottom Line: Phylogenetically, highest similarity was to Streptomyces misionensis NBRC 13063(T) (99.6%) along with two other strains (>98.9% sequence similarities).The DNA-DNA relatedness between MUSC 136(T) and these type strains ranged from 22.7 ± 0.5% to 46.5 ± 0.2%.It was also found to possess high cytotoxic effect against HCT-116 cells, which probably mediated through altering p53 protein and intracellular glutathione levels.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Monash University Malaysia, 47500 Bandar Sunway, Malaysia.

ABSTRACT
Actinobacteria from the unique intertidal ecosystem of the mangroves are known to produce novel, bioactive secondary metabolites. A novel strain known as MUSC 136(T) (=DSM 100712(T) = MCCC 1K01246(T)) which was isolated from Malaysian mangrove forest soil has proven to be no exception. Assessed by a polyphasic approach, its taxonomy showed a range of phylogenetic and chemotaxonomic properties consistent with the genus of Streptomyces. Phylogenetically, highest similarity was to Streptomyces misionensis NBRC 13063(T) (99.6%) along with two other strains (>98.9% sequence similarities). The DNA-DNA relatedness between MUSC 136(T) and these type strains ranged from 22.7 ± 0.5% to 46.5 ± 0.2%. Overall, polyphasic approach studies indicated this strain represents a novel species, for which the name Streptomyces malaysiense sp. nov. is proposed. The potential bioactivities of this strain were explored by means of antioxidant and cytotoxic assays. Intriguingly, MUSC 136(T) exhibited strong antioxidative activities as evaluated by a panel of antioxidant assays. It was also found to possess high cytotoxic effect against HCT-116 cells, which probably mediated through altering p53 protein and intracellular glutathione levels. Chemical analysis of the extract using GC-MS further affirms that the strain produces chemopreventive related metabolites.

No MeSH data available.


Related in: MedlinePlus