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Streptomyces malaysiense sp. nov.: A novel Malaysian mangrove soil actinobacterium with antioxidative activity and cytotoxic potential against human cancer cell lines.

Ser HL, Palanisamy UD, Yin WF, Chan KG, Goh BH, Lee LH - Sci Rep (2016)

Bottom Line: Phylogenetically, highest similarity was to Streptomyces misionensis NBRC 13063(T) (99.6%) along with two other strains (>98.9% sequence similarities).The DNA-DNA relatedness between MUSC 136(T) and these type strains ranged from 22.7 ± 0.5% to 46.5 ± 0.2%.It was also found to possess high cytotoxic effect against HCT-116 cells, which probably mediated through altering p53 protein and intracellular glutathione levels.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Monash University Malaysia, 47500 Bandar Sunway, Malaysia.

ABSTRACT
Actinobacteria from the unique intertidal ecosystem of the mangroves are known to produce novel, bioactive secondary metabolites. A novel strain known as MUSC 136(T) (=DSM 100712(T) = MCCC 1K01246(T)) which was isolated from Malaysian mangrove forest soil has proven to be no exception. Assessed by a polyphasic approach, its taxonomy showed a range of phylogenetic and chemotaxonomic properties consistent with the genus of Streptomyces. Phylogenetically, highest similarity was to Streptomyces misionensis NBRC 13063(T) (99.6%) along with two other strains (>98.9% sequence similarities). The DNA-DNA relatedness between MUSC 136(T) and these type strains ranged from 22.7 ± 0.5% to 46.5 ± 0.2%. Overall, polyphasic approach studies indicated this strain represents a novel species, for which the name Streptomyces malaysiense sp. nov. is proposed. The potential bioactivities of this strain were explored by means of antioxidant and cytotoxic assays. Intriguingly, MUSC 136(T) exhibited strong antioxidative activities as evaluated by a panel of antioxidant assays. It was also found to possess high cytotoxic effect against HCT-116 cells, which probably mediated through altering p53 protein and intracellular glutathione levels. Chemical analysis of the extract using GC-MS further affirms that the strain produces chemopreventive related metabolites.

No MeSH data available.


Related in: MedlinePlus

Scanning electron microscope of Streptomyces malaysiense MUSC 136T.
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f1: Scanning electron microscope of Streptomyces malaysiense MUSC 136T.

Mentions: Strain MUSC 136T grew well on ISP 2, ISP 5, ISP 6, ISP 7 agar, actinomycetes isolation agar, nutrient agar and starch casein agar after 7–14 days at 28 °C, moderately on Streptomyces agar and ISP 3 agar, and not at all on ISP 4 agar. The colors of the aerial and substrate mycelium were media-dependent (See Supplementary Table S1). The morphological observation of a 15-day-old culture grown on ISP 2 agar revealed abundant growth of both aerial and vegetative hyphae, which were well developed and not fragmented. These morphological features are consistent with assignment of the strain to the genus Streptomyces18 (Fig. 1, Table 1). Growth was found to occur at pH 6.0–7.0 (optimum pH 7.0), with 0–6% NaCl tolerance (optimum 0–2%) and at 26–40 °C (optimum 28–32 °C). Cells were found to be positive for catalase but negative for hemolytic activity and melanoid pigment production. Hydrolysis of soluble starch, tributyrin (lipase) and casein were found to be positive, but negative for hydrolysis of carboxymethylcellulose, chitin and xylan. Strain MUSC 136T can be differentiated from closely related members of the genus Streptomyces using a range of phenotypic properties (Table 1). In chemical sensitivity assays, cells are resistant to aztreonam, fusidic acid, guanine HCl, lincomycin, lithium chloride, minocycline, nalidixic acid, niaproof 4, potassium tellurite, rifamycin RV, sodium bromate, sodium butyrate, 1% sodium lactate, tetrazolium blue, tetrazolium violet, troleandomycin and vancomycin.


Streptomyces malaysiense sp. nov.: A novel Malaysian mangrove soil actinobacterium with antioxidative activity and cytotoxic potential against human cancer cell lines.

Ser HL, Palanisamy UD, Yin WF, Chan KG, Goh BH, Lee LH - Sci Rep (2016)

Scanning electron microscope of Streptomyces malaysiense MUSC 136T.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829849&req=5

f1: Scanning electron microscope of Streptomyces malaysiense MUSC 136T.
Mentions: Strain MUSC 136T grew well on ISP 2, ISP 5, ISP 6, ISP 7 agar, actinomycetes isolation agar, nutrient agar and starch casein agar after 7–14 days at 28 °C, moderately on Streptomyces agar and ISP 3 agar, and not at all on ISP 4 agar. The colors of the aerial and substrate mycelium were media-dependent (See Supplementary Table S1). The morphological observation of a 15-day-old culture grown on ISP 2 agar revealed abundant growth of both aerial and vegetative hyphae, which were well developed and not fragmented. These morphological features are consistent with assignment of the strain to the genus Streptomyces18 (Fig. 1, Table 1). Growth was found to occur at pH 6.0–7.0 (optimum pH 7.0), with 0–6% NaCl tolerance (optimum 0–2%) and at 26–40 °C (optimum 28–32 °C). Cells were found to be positive for catalase but negative for hemolytic activity and melanoid pigment production. Hydrolysis of soluble starch, tributyrin (lipase) and casein were found to be positive, but negative for hydrolysis of carboxymethylcellulose, chitin and xylan. Strain MUSC 136T can be differentiated from closely related members of the genus Streptomyces using a range of phenotypic properties (Table 1). In chemical sensitivity assays, cells are resistant to aztreonam, fusidic acid, guanine HCl, lincomycin, lithium chloride, minocycline, nalidixic acid, niaproof 4, potassium tellurite, rifamycin RV, sodium bromate, sodium butyrate, 1% sodium lactate, tetrazolium blue, tetrazolium violet, troleandomycin and vancomycin.

Bottom Line: Phylogenetically, highest similarity was to Streptomyces misionensis NBRC 13063(T) (99.6%) along with two other strains (>98.9% sequence similarities).The DNA-DNA relatedness between MUSC 136(T) and these type strains ranged from 22.7 ± 0.5% to 46.5 ± 0.2%.It was also found to possess high cytotoxic effect against HCT-116 cells, which probably mediated through altering p53 protein and intracellular glutathione levels.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, Monash University Malaysia, 47500 Bandar Sunway, Malaysia.

ABSTRACT
Actinobacteria from the unique intertidal ecosystem of the mangroves are known to produce novel, bioactive secondary metabolites. A novel strain known as MUSC 136(T) (=DSM 100712(T) = MCCC 1K01246(T)) which was isolated from Malaysian mangrove forest soil has proven to be no exception. Assessed by a polyphasic approach, its taxonomy showed a range of phylogenetic and chemotaxonomic properties consistent with the genus of Streptomyces. Phylogenetically, highest similarity was to Streptomyces misionensis NBRC 13063(T) (99.6%) along with two other strains (>98.9% sequence similarities). The DNA-DNA relatedness between MUSC 136(T) and these type strains ranged from 22.7 ± 0.5% to 46.5 ± 0.2%. Overall, polyphasic approach studies indicated this strain represents a novel species, for which the name Streptomyces malaysiense sp. nov. is proposed. The potential bioactivities of this strain were explored by means of antioxidant and cytotoxic assays. Intriguingly, MUSC 136(T) exhibited strong antioxidative activities as evaluated by a panel of antioxidant assays. It was also found to possess high cytotoxic effect against HCT-116 cells, which probably mediated through altering p53 protein and intracellular glutathione levels. Chemical analysis of the extract using GC-MS further affirms that the strain produces chemopreventive related metabolites.

No MeSH data available.


Related in: MedlinePlus