Limits...
In vitro antibacterial activity of ZnO and Nd doped ZnO nanoparticles against ESBL producing Escherichia coli and Klebsiella pneumoniae.

Hameed AS, Karthikeyan C, Ahamed AP, Thajuddin N, Alharbi NS, Alharbi SA, Ravi G - Sci Rep (2016)

Bottom Line: The FT-IR spectra confirmed the Zn-O stretching bands at 422 and 451 cm(-1) for ZnO and Nd doped ZnO NPs respectively.From the UV-VIS spectroscopic measurement, the excitonic peaks were found around 373 nm and 380 nm for the respective samples.From confocal laser scanning microscopic (CLSM) analysis, the apoptotic nature of the cells was confirmed by the cell shrinkage, disorganization of cell wall and cell membrane and dead cell of the bacteria.

View Article: PubMed Central - PubMed

Affiliation: PG and Research Department of Physics, Jamal Mohamed College, Tiruchirappalli-620020, Tamil Nadu, India.

ABSTRACT
Pure ZnO and Neodymium (Nd) doped ZnO nanoparticles (NPs) were synthesized by the co-precipitation method. The synthesized nanoparticles retained the wurtzite hexagonal structure. From FESEM studies, ZnO and Nd doped ZnO NPs showed nanorod and nanoflower like morphology respectively. The FT-IR spectra confirmed the Zn-O stretching bands at 422 and 451 cm(-1) for ZnO and Nd doped ZnO NPs respectively. From the UV-VIS spectroscopic measurement, the excitonic peaks were found around 373 nm and 380 nm for the respective samples. The photoluminescence measurements revealed that the broad emission was composed of ten different bands due to zinc vacancies, oxygen vacancies and surface defects. The antibacterial studies performed against extended spectrum β-lactamases (ESBLs) producing strains of Escherichia coli and Klebsiella pneumoniae showed that the Nd doped ZnO NPs possessed a greater antibacterial effect than the pure ZnO NPs. From confocal laser scanning microscopic (CLSM) analysis, the apoptotic nature of the cells was confirmed by the cell shrinkage, disorganization of cell wall and cell membrane and dead cell of the bacteria. SEM analysis revealed the existence of bacterial loss of viability due to an impairment of cell membrane integrity, which was highly consistent with the damage of cell walls.

No MeSH data available.


Related in: MedlinePlus

MBC results for the entire test against ESBLs producing (a) E. coli treated with ZnO NPs, (b) K. pneumoniae treated with ZnO NPs, (c) E. coli treated with Nd doped ZnO NPs (d) K. pneumoniae treated with Nd doped ZnO NPs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4829841&req=5

f7: MBC results for the entire test against ESBLs producing (a) E. coli treated with ZnO NPs, (b) K. pneumoniae treated with ZnO NPs, (c) E. coli treated with Nd doped ZnO NPs (d) K. pneumoniae treated with Nd doped ZnO NPs.

Mentions: Generally, bactericidal agents are much preferred in the clinical field because bactericides lead to rapid and better recovery from bacterial infections and also minimize the possibility of the emergence of drug resistance51. Since the undoped and Nd doped ZnO NPs exhibited relatively superior activity against all the pathogens, its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) are ascertained. The MIC and MBC results for all of the test pathogens are shown in Fig. 7(a,d). Table 2 shows a comparison between present and reported MIC values of various metals doped ZnO NPs required to inhibit the growth of E. coli. Table 3 shows the MIC results for the entire test against ESBLs producing E. coli and K. pneumoniae for pure ZnO and Nd doped ZnO NPs. Table 3 showed that the control bacterial culture possessed 100% of cell growth. While increasing the concentration (50, 150, 250, 350, 500, 650, 800 and 1000 μg/mL) of ZnO and Nd doped ZnO NPs, the bacterial cell growth percentage decreased. The 800 μg/mL of Nd doped ZnO samples treated with E. coli strain resulted 100% of cell death as shown in Table 3. Thus, we conclude that from the present study, the minimal quantity of the Nd-doped ZnO NPs sample required to inhibit the bacterial growth is found to be 800 μg/mL for E. coli, which is mentioned in Table 2 for comparison.


In vitro antibacterial activity of ZnO and Nd doped ZnO nanoparticles against ESBL producing Escherichia coli and Klebsiella pneumoniae.

Hameed AS, Karthikeyan C, Ahamed AP, Thajuddin N, Alharbi NS, Alharbi SA, Ravi G - Sci Rep (2016)

MBC results for the entire test against ESBLs producing (a) E. coli treated with ZnO NPs, (b) K. pneumoniae treated with ZnO NPs, (c) E. coli treated with Nd doped ZnO NPs (d) K. pneumoniae treated with Nd doped ZnO NPs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829841&req=5

f7: MBC results for the entire test against ESBLs producing (a) E. coli treated with ZnO NPs, (b) K. pneumoniae treated with ZnO NPs, (c) E. coli treated with Nd doped ZnO NPs (d) K. pneumoniae treated with Nd doped ZnO NPs.
Mentions: Generally, bactericidal agents are much preferred in the clinical field because bactericides lead to rapid and better recovery from bacterial infections and also minimize the possibility of the emergence of drug resistance51. Since the undoped and Nd doped ZnO NPs exhibited relatively superior activity against all the pathogens, its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) are ascertained. The MIC and MBC results for all of the test pathogens are shown in Fig. 7(a,d). Table 2 shows a comparison between present and reported MIC values of various metals doped ZnO NPs required to inhibit the growth of E. coli. Table 3 shows the MIC results for the entire test against ESBLs producing E. coli and K. pneumoniae for pure ZnO and Nd doped ZnO NPs. Table 3 showed that the control bacterial culture possessed 100% of cell growth. While increasing the concentration (50, 150, 250, 350, 500, 650, 800 and 1000 μg/mL) of ZnO and Nd doped ZnO NPs, the bacterial cell growth percentage decreased. The 800 μg/mL of Nd doped ZnO samples treated with E. coli strain resulted 100% of cell death as shown in Table 3. Thus, we conclude that from the present study, the minimal quantity of the Nd-doped ZnO NPs sample required to inhibit the bacterial growth is found to be 800 μg/mL for E. coli, which is mentioned in Table 2 for comparison.

Bottom Line: The FT-IR spectra confirmed the Zn-O stretching bands at 422 and 451 cm(-1) for ZnO and Nd doped ZnO NPs respectively.From the UV-VIS spectroscopic measurement, the excitonic peaks were found around 373 nm and 380 nm for the respective samples.From confocal laser scanning microscopic (CLSM) analysis, the apoptotic nature of the cells was confirmed by the cell shrinkage, disorganization of cell wall and cell membrane and dead cell of the bacteria.

View Article: PubMed Central - PubMed

Affiliation: PG and Research Department of Physics, Jamal Mohamed College, Tiruchirappalli-620020, Tamil Nadu, India.

ABSTRACT
Pure ZnO and Neodymium (Nd) doped ZnO nanoparticles (NPs) were synthesized by the co-precipitation method. The synthesized nanoparticles retained the wurtzite hexagonal structure. From FESEM studies, ZnO and Nd doped ZnO NPs showed nanorod and nanoflower like morphology respectively. The FT-IR spectra confirmed the Zn-O stretching bands at 422 and 451 cm(-1) for ZnO and Nd doped ZnO NPs respectively. From the UV-VIS spectroscopic measurement, the excitonic peaks were found around 373 nm and 380 nm for the respective samples. The photoluminescence measurements revealed that the broad emission was composed of ten different bands due to zinc vacancies, oxygen vacancies and surface defects. The antibacterial studies performed against extended spectrum β-lactamases (ESBLs) producing strains of Escherichia coli and Klebsiella pneumoniae showed that the Nd doped ZnO NPs possessed a greater antibacterial effect than the pure ZnO NPs. From confocal laser scanning microscopic (CLSM) analysis, the apoptotic nature of the cells was confirmed by the cell shrinkage, disorganization of cell wall and cell membrane and dead cell of the bacteria. SEM analysis revealed the existence of bacterial loss of viability due to an impairment of cell membrane integrity, which was highly consistent with the damage of cell walls.

No MeSH data available.


Related in: MedlinePlus