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In vitro antibacterial activity of ZnO and Nd doped ZnO nanoparticles against ESBL producing Escherichia coli and Klebsiella pneumoniae.

Hameed AS, Karthikeyan C, Ahamed AP, Thajuddin N, Alharbi NS, Alharbi SA, Ravi G - Sci Rep (2016)

Bottom Line: The FT-IR spectra confirmed the Zn-O stretching bands at 422 and 451 cm(-1) for ZnO and Nd doped ZnO NPs respectively.From the UV-VIS spectroscopic measurement, the excitonic peaks were found around 373 nm and 380 nm for the respective samples.From confocal laser scanning microscopic (CLSM) analysis, the apoptotic nature of the cells was confirmed by the cell shrinkage, disorganization of cell wall and cell membrane and dead cell of the bacteria.

View Article: PubMed Central - PubMed

Affiliation: PG and Research Department of Physics, Jamal Mohamed College, Tiruchirappalli-620020, Tamil Nadu, India.

ABSTRACT
Pure ZnO and Neodymium (Nd) doped ZnO nanoparticles (NPs) were synthesized by the co-precipitation method. The synthesized nanoparticles retained the wurtzite hexagonal structure. From FESEM studies, ZnO and Nd doped ZnO NPs showed nanorod and nanoflower like morphology respectively. The FT-IR spectra confirmed the Zn-O stretching bands at 422 and 451 cm(-1) for ZnO and Nd doped ZnO NPs respectively. From the UV-VIS spectroscopic measurement, the excitonic peaks were found around 373 nm and 380 nm for the respective samples. The photoluminescence measurements revealed that the broad emission was composed of ten different bands due to zinc vacancies, oxygen vacancies and surface defects. The antibacterial studies performed against extended spectrum β-lactamases (ESBLs) producing strains of Escherichia coli and Klebsiella pneumoniae showed that the Nd doped ZnO NPs possessed a greater antibacterial effect than the pure ZnO NPs. From confocal laser scanning microscopic (CLSM) analysis, the apoptotic nature of the cells was confirmed by the cell shrinkage, disorganization of cell wall and cell membrane and dead cell of the bacteria. SEM analysis revealed the existence of bacterial loss of viability due to an impairment of cell membrane integrity, which was highly consistent with the damage of cell walls.

No MeSH data available.


Related in: MedlinePlus

E-Test by triple detection strip- clear cut ESBL positive ceftazidime, cefotaxime, cefepime (Mix)/ceftazidime, cefotaxime, cefepime and clavulanic acid (Mix+) MIC value R/0.38 mm = >8 mm.
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f4: E-Test by triple detection strip- clear cut ESBL positive ceftazidime, cefotaxime, cefepime (Mix)/ceftazidime, cefotaxime, cefepime and clavulanic acid (Mix+) MIC value R/0.38 mm = >8 mm.

Mentions: The two isolates tested for their resistance pattern in this E. coli were susceptible to novobiocin, rifampicin and erythromycin, which affirmed its resistance to remaining tested antibiotics. The strain K. pneumonia showed 100% of resistance to all the tested antibiotics and was susceptible only to amikacin. Applying CLSI ESBL typing of E. coli and K. pneumonia (Table 1), in the combination disc method showed that the isolates had “positive” result (Fig. 3). According to CLSI ESBL typing44 (triple detection strip), MIC criteria showed similar results (Fig. 4). Instead of cepfodoxime, fourth generation cepefime was used in the strip test as a marker for high level ESBL production.


In vitro antibacterial activity of ZnO and Nd doped ZnO nanoparticles against ESBL producing Escherichia coli and Klebsiella pneumoniae.

Hameed AS, Karthikeyan C, Ahamed AP, Thajuddin N, Alharbi NS, Alharbi SA, Ravi G - Sci Rep (2016)

E-Test by triple detection strip- clear cut ESBL positive ceftazidime, cefotaxime, cefepime (Mix)/ceftazidime, cefotaxime, cefepime and clavulanic acid (Mix+) MIC value R/0.38 mm = >8 mm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829841&req=5

f4: E-Test by triple detection strip- clear cut ESBL positive ceftazidime, cefotaxime, cefepime (Mix)/ceftazidime, cefotaxime, cefepime and clavulanic acid (Mix+) MIC value R/0.38 mm = >8 mm.
Mentions: The two isolates tested for their resistance pattern in this E. coli were susceptible to novobiocin, rifampicin and erythromycin, which affirmed its resistance to remaining tested antibiotics. The strain K. pneumonia showed 100% of resistance to all the tested antibiotics and was susceptible only to amikacin. Applying CLSI ESBL typing of E. coli and K. pneumonia (Table 1), in the combination disc method showed that the isolates had “positive” result (Fig. 3). According to CLSI ESBL typing44 (triple detection strip), MIC criteria showed similar results (Fig. 4). Instead of cepfodoxime, fourth generation cepefime was used in the strip test as a marker for high level ESBL production.

Bottom Line: The FT-IR spectra confirmed the Zn-O stretching bands at 422 and 451 cm(-1) for ZnO and Nd doped ZnO NPs respectively.From the UV-VIS spectroscopic measurement, the excitonic peaks were found around 373 nm and 380 nm for the respective samples.From confocal laser scanning microscopic (CLSM) analysis, the apoptotic nature of the cells was confirmed by the cell shrinkage, disorganization of cell wall and cell membrane and dead cell of the bacteria.

View Article: PubMed Central - PubMed

Affiliation: PG and Research Department of Physics, Jamal Mohamed College, Tiruchirappalli-620020, Tamil Nadu, India.

ABSTRACT
Pure ZnO and Neodymium (Nd) doped ZnO nanoparticles (NPs) were synthesized by the co-precipitation method. The synthesized nanoparticles retained the wurtzite hexagonal structure. From FESEM studies, ZnO and Nd doped ZnO NPs showed nanorod and nanoflower like morphology respectively. The FT-IR spectra confirmed the Zn-O stretching bands at 422 and 451 cm(-1) for ZnO and Nd doped ZnO NPs respectively. From the UV-VIS spectroscopic measurement, the excitonic peaks were found around 373 nm and 380 nm for the respective samples. The photoluminescence measurements revealed that the broad emission was composed of ten different bands due to zinc vacancies, oxygen vacancies and surface defects. The antibacterial studies performed against extended spectrum β-lactamases (ESBLs) producing strains of Escherichia coli and Klebsiella pneumoniae showed that the Nd doped ZnO NPs possessed a greater antibacterial effect than the pure ZnO NPs. From confocal laser scanning microscopic (CLSM) analysis, the apoptotic nature of the cells was confirmed by the cell shrinkage, disorganization of cell wall and cell membrane and dead cell of the bacteria. SEM analysis revealed the existence of bacterial loss of viability due to an impairment of cell membrane integrity, which was highly consistent with the damage of cell walls.

No MeSH data available.


Related in: MedlinePlus