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In vitro antibacterial activity of ZnO and Nd doped ZnO nanoparticles against ESBL producing Escherichia coli and Klebsiella pneumoniae.

Hameed AS, Karthikeyan C, Ahamed AP, Thajuddin N, Alharbi NS, Alharbi SA, Ravi G - Sci Rep (2016)

Bottom Line: The FT-IR spectra confirmed the Zn-O stretching bands at 422 and 451 cm(-1) for ZnO and Nd doped ZnO NPs respectively.From the UV-VIS spectroscopic measurement, the excitonic peaks were found around 373 nm and 380 nm for the respective samples.From confocal laser scanning microscopic (CLSM) analysis, the apoptotic nature of the cells was confirmed by the cell shrinkage, disorganization of cell wall and cell membrane and dead cell of the bacteria.

View Article: PubMed Central - PubMed

Affiliation: PG and Research Department of Physics, Jamal Mohamed College, Tiruchirappalli-620020, Tamil Nadu, India.

ABSTRACT
Pure ZnO and Neodymium (Nd) doped ZnO nanoparticles (NPs) were synthesized by the co-precipitation method. The synthesized nanoparticles retained the wurtzite hexagonal structure. From FESEM studies, ZnO and Nd doped ZnO NPs showed nanorod and nanoflower like morphology respectively. The FT-IR spectra confirmed the Zn-O stretching bands at 422 and 451 cm(-1) for ZnO and Nd doped ZnO NPs respectively. From the UV-VIS spectroscopic measurement, the excitonic peaks were found around 373 nm and 380 nm for the respective samples. The photoluminescence measurements revealed that the broad emission was composed of ten different bands due to zinc vacancies, oxygen vacancies and surface defects. The antibacterial studies performed against extended spectrum β-lactamases (ESBLs) producing strains of Escherichia coli and Klebsiella pneumoniae showed that the Nd doped ZnO NPs possessed a greater antibacterial effect than the pure ZnO NPs. From confocal laser scanning microscopic (CLSM) analysis, the apoptotic nature of the cells was confirmed by the cell shrinkage, disorganization of cell wall and cell membrane and dead cell of the bacteria. SEM analysis revealed the existence of bacterial loss of viability due to an impairment of cell membrane integrity, which was highly consistent with the damage of cell walls.

No MeSH data available.


Related in: MedlinePlus

SEM image of (a) Control, (b) E. coli and (c) K. pneumoniae treated with MIC of Nd doped ZnO NPs slurries for 4 h.
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f10: SEM image of (a) Control, (b) E. coli and (c) K. pneumoniae treated with MIC of Nd doped ZnO NPs slurries for 4 h.

Mentions: Figure 10(a–c) shows that the activity of Nd doped ZnO NPs on the bacterial cell and both bacterial strains were examined using SEM analysis to look for structural changes in outer-membrane of the cells. Figure 10a shows the untreated control cell marked by a circle and these cells of bacterial species did not exhibit injury to the cell membrane. In the case of Nd doped ZnO NPs treated with E. coli, the cells are marked by the ellipses (Fig. 10b) whereas for the Nd doped ZnO NPs treated with K. pneumoniae, the cells are marked by parallel straight lines (Fig. 10c). The Nd doped ZnO NPs are on the surface of bacteria, leading to the disruption and disorganization of membranes. The bacterial loss of viability is correlated to an impairment of cell membrane integrity, which is highly consistent with the damage cell walls for both strains, revealed by the SEM analysis.


In vitro antibacterial activity of ZnO and Nd doped ZnO nanoparticles against ESBL producing Escherichia coli and Klebsiella pneumoniae.

Hameed AS, Karthikeyan C, Ahamed AP, Thajuddin N, Alharbi NS, Alharbi SA, Ravi G - Sci Rep (2016)

SEM image of (a) Control, (b) E. coli and (c) K. pneumoniae treated with MIC of Nd doped ZnO NPs slurries for 4 h.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829841&req=5

f10: SEM image of (a) Control, (b) E. coli and (c) K. pneumoniae treated with MIC of Nd doped ZnO NPs slurries for 4 h.
Mentions: Figure 10(a–c) shows that the activity of Nd doped ZnO NPs on the bacterial cell and both bacterial strains were examined using SEM analysis to look for structural changes in outer-membrane of the cells. Figure 10a shows the untreated control cell marked by a circle and these cells of bacterial species did not exhibit injury to the cell membrane. In the case of Nd doped ZnO NPs treated with E. coli, the cells are marked by the ellipses (Fig. 10b) whereas for the Nd doped ZnO NPs treated with K. pneumoniae, the cells are marked by parallel straight lines (Fig. 10c). The Nd doped ZnO NPs are on the surface of bacteria, leading to the disruption and disorganization of membranes. The bacterial loss of viability is correlated to an impairment of cell membrane integrity, which is highly consistent with the damage cell walls for both strains, revealed by the SEM analysis.

Bottom Line: The FT-IR spectra confirmed the Zn-O stretching bands at 422 and 451 cm(-1) for ZnO and Nd doped ZnO NPs respectively.From the UV-VIS spectroscopic measurement, the excitonic peaks were found around 373 nm and 380 nm for the respective samples.From confocal laser scanning microscopic (CLSM) analysis, the apoptotic nature of the cells was confirmed by the cell shrinkage, disorganization of cell wall and cell membrane and dead cell of the bacteria.

View Article: PubMed Central - PubMed

Affiliation: PG and Research Department of Physics, Jamal Mohamed College, Tiruchirappalli-620020, Tamil Nadu, India.

ABSTRACT
Pure ZnO and Neodymium (Nd) doped ZnO nanoparticles (NPs) were synthesized by the co-precipitation method. The synthesized nanoparticles retained the wurtzite hexagonal structure. From FESEM studies, ZnO and Nd doped ZnO NPs showed nanorod and nanoflower like morphology respectively. The FT-IR spectra confirmed the Zn-O stretching bands at 422 and 451 cm(-1) for ZnO and Nd doped ZnO NPs respectively. From the UV-VIS spectroscopic measurement, the excitonic peaks were found around 373 nm and 380 nm for the respective samples. The photoluminescence measurements revealed that the broad emission was composed of ten different bands due to zinc vacancies, oxygen vacancies and surface defects. The antibacterial studies performed against extended spectrum β-lactamases (ESBLs) producing strains of Escherichia coli and Klebsiella pneumoniae showed that the Nd doped ZnO NPs possessed a greater antibacterial effect than the pure ZnO NPs. From confocal laser scanning microscopic (CLSM) analysis, the apoptotic nature of the cells was confirmed by the cell shrinkage, disorganization of cell wall and cell membrane and dead cell of the bacteria. SEM analysis revealed the existence of bacterial loss of viability due to an impairment of cell membrane integrity, which was highly consistent with the damage of cell walls.

No MeSH data available.


Related in: MedlinePlus