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Increased N6-methyladenosine in Human Sperm RNA as a Risk Factor for Asthenozoospermia.

Yang Y, Huang W, Huang JT, Shen F, Xiong J, Yuan EF, Qin SS, Zhang M, Feng YQ, Yuan BF, Liu SM - Sci Rep (2016)

Bottom Line: We found that m(6)A content (p = 0.033) and the mRNA expression of METTL3 (p = 0.016) and METTL14 (p = 0.025) in asthenozoospermia patients were significantly higher than those of controls.Increased m(6)A content was a risk factor for asthenozoospermia (odds ratio (OR) 3.229, 95% confidence interval (CI) 1.178 - 8.853, p = 0.023).Our data suggest that increased m(6)A content is a risk factor for asthenozoospermia and affects sperm motility.

View Article: PubMed Central - PubMed

Affiliation: Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Donghu Road 169#, Wuhan, 430071, P.R. China.

ABSTRACT
Male infertility is a worldwide medical problem. Asthenozoospermia is a common cause of infertility. Epigenetic modifications of DNA and histones have been shown to influence human infertility, but no research has explored whether N(6)-methyladenosine (m(6)A) level in RNA is associated with asthenozoospermia. Here, we collected a total of 52 semen samples, including 20 asthenozoospermia patients and 32 healthy controls. An LC-ESI-MS/MS method was used to detect m(6)A contents in sperm RNA, and real-time PCR was performed to determine the mRNA expression of demethylase (FTO, ALKBH5), methyltransferase (METTL3, METTL14, WTAP) and an m(6)A-selective-binding protein (YTHDF2). We found that m(6)A content (p = 0.033) and the mRNA expression of METTL3 (p = 0.016) and METTL14 (p = 0.025) in asthenozoospermia patients were significantly higher than those of controls. Increased m(6)A content was a risk factor for asthenozoospermia (odds ratio (OR) 3.229, 95% confidence interval (CI) 1.178 - 8.853, p = 0.023). Moreover, m(6)A content was correlated with the expression of METTL3 (r = 0.303, p = 0.032) and with sperm motility (progressive motility: r = -0.288, p = 0.038; non-progressive motility: r = -0.293, p = 0.037; immotility: r = 0.387, p = 0.005). Our data suggest that increased m(6)A content is a risk factor for asthenozoospermia and affects sperm motility. Methyltransferases, particularly METTL3, play key roles in increasing m(6)A contents in sperm RNA.

No MeSH data available.


Related in: MedlinePlus

A process of the dynamic m6A modification in asthenozoospermia.Increased mRNA expression of METTL3 and METTL14 contributes to the elevation of m6A content which affects sperm motility and further results in male infertility.
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f3: A process of the dynamic m6A modification in asthenozoospermia.Increased mRNA expression of METTL3 and METTL14 contributes to the elevation of m6A content which affects sperm motility and further results in male infertility.

Mentions: In summary, we first reported that methyltransferases (METTL3 and METTL14), particularly METTL3, play key roles in the dynamic modification of m6A in asthenozoospermia patients and that increased m6A can impair sperm motility. According to our results, we deduce a process of dynamic modification of m6A in asthenozoospermia patients (Fig. 3). The underlying mechanisms of m6A modification in asthenozoospermia still need further investigation.


Increased N6-methyladenosine in Human Sperm RNA as a Risk Factor for Asthenozoospermia.

Yang Y, Huang W, Huang JT, Shen F, Xiong J, Yuan EF, Qin SS, Zhang M, Feng YQ, Yuan BF, Liu SM - Sci Rep (2016)

A process of the dynamic m6A modification in asthenozoospermia.Increased mRNA expression of METTL3 and METTL14 contributes to the elevation of m6A content which affects sperm motility and further results in male infertility.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829835&req=5

f3: A process of the dynamic m6A modification in asthenozoospermia.Increased mRNA expression of METTL3 and METTL14 contributes to the elevation of m6A content which affects sperm motility and further results in male infertility.
Mentions: In summary, we first reported that methyltransferases (METTL3 and METTL14), particularly METTL3, play key roles in the dynamic modification of m6A in asthenozoospermia patients and that increased m6A can impair sperm motility. According to our results, we deduce a process of dynamic modification of m6A in asthenozoospermia patients (Fig. 3). The underlying mechanisms of m6A modification in asthenozoospermia still need further investigation.

Bottom Line: We found that m(6)A content (p = 0.033) and the mRNA expression of METTL3 (p = 0.016) and METTL14 (p = 0.025) in asthenozoospermia patients were significantly higher than those of controls.Increased m(6)A content was a risk factor for asthenozoospermia (odds ratio (OR) 3.229, 95% confidence interval (CI) 1.178 - 8.853, p = 0.023).Our data suggest that increased m(6)A content is a risk factor for asthenozoospermia and affects sperm motility.

View Article: PubMed Central - PubMed

Affiliation: Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Donghu Road 169#, Wuhan, 430071, P.R. China.

ABSTRACT
Male infertility is a worldwide medical problem. Asthenozoospermia is a common cause of infertility. Epigenetic modifications of DNA and histones have been shown to influence human infertility, but no research has explored whether N(6)-methyladenosine (m(6)A) level in RNA is associated with asthenozoospermia. Here, we collected a total of 52 semen samples, including 20 asthenozoospermia patients and 32 healthy controls. An LC-ESI-MS/MS method was used to detect m(6)A contents in sperm RNA, and real-time PCR was performed to determine the mRNA expression of demethylase (FTO, ALKBH5), methyltransferase (METTL3, METTL14, WTAP) and an m(6)A-selective-binding protein (YTHDF2). We found that m(6)A content (p = 0.033) and the mRNA expression of METTL3 (p = 0.016) and METTL14 (p = 0.025) in asthenozoospermia patients were significantly higher than those of controls. Increased m(6)A content was a risk factor for asthenozoospermia (odds ratio (OR) 3.229, 95% confidence interval (CI) 1.178 - 8.853, p = 0.023). Moreover, m(6)A content was correlated with the expression of METTL3 (r = 0.303, p = 0.032) and with sperm motility (progressive motility: r = -0.288, p = 0.038; non-progressive motility: r = -0.293, p = 0.037; immotility: r = 0.387, p = 0.005). Our data suggest that increased m(6)A content is a risk factor for asthenozoospermia and affects sperm motility. Methyltransferases, particularly METTL3, play key roles in increasing m(6)A contents in sperm RNA.

No MeSH data available.


Related in: MedlinePlus