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The role of VipAlbumin(®) as an immunostimulatory agent for controlling homeostasis and proliferation of lymphoid cells.

Dwijayanti DR, Djati MS, Rifa'I M - Cent Eur J Immunol (2016)

Bottom Line: The concentrations of VipAlbumin (®) used were 0 µg/ml; 0.33 µg/ml; 33.3 µg/ml; and 3333.3 µg/ml.VipAlbumin can significantly increase the relative number of CD4(+)CD62L(+) T cell, regulatory T cell, and B220+ cell (p < 0.05) compared to control.This study gives scientific evidence that VipAlbumin can be used as an immunostimulant which accelerates immunocompetent cells growth.

View Article: PubMed Central - PubMed

Affiliation: Biology Department, Brawijaya University, Malang, Indonesia.

ABSTRACT
VipAlbumin(®) is a supplement from snakehead fish (Ophiocephalus striatus) which has high content of albumin that is very important to develop new cells. The aims of this study were to know the effect of VipAlbumin(®) to cell proliferation, expression level of CD4(+)CD62L(+) T cell, regulatory T cell, and B220+ cell, and immunocompetent cell cycle. Cell isolated from spleen of pathogen free mice were cultured in RPMI 1640 with 10% FBS, 1% Pen/Strep 10×, 2-Mercaptoetanol, anti-CD3 and LPS. The concentrations of VipAlbumin (®) used were 0 µg/ml; 0.33 µg/ml; 33.3 µg/ml; and 3333.3 µg/ml. The cell was incubated in CO2 5% incubator 37°C for 3 days for cell cycle and 5 days for proliferation analysis and cell expression. FACS analysis was done to know cell proliferation profile, status of cell, and cell cycle. Concentration 33.3 µg/ml and 3333.3 µg/ml significantly can increase cell proliferation and induce cell enter G2/M phase (p < 0.05) compared to control. VipAlbumin can significantly increase the relative number of CD4(+)CD62L(+) T cell, regulatory T cell, and B220+ cell (p < 0.05) compared to control. This study gives scientific evidence that VipAlbumin can be used as an immunostimulant which accelerates immunocompetent cells growth.

No MeSH data available.


Related in: MedlinePlus

Stimulation cells from spleen by using VipAlbumin® for 5 days showed the increase of cell proliferation relative number. Spleen cells were cultured in RPMI medium with 10% FBS, anti-CD3 and LPS for five days. On day 5, cell cultures were harvested and analyzed by flow cytometry and tabulated into Microsoft Excel. The cell proliferation was presented in relative number and obtained from all living cells. Data were mean ± SD in each group with p value ≤ 0.05
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Figure 0001: Stimulation cells from spleen by using VipAlbumin® for 5 days showed the increase of cell proliferation relative number. Spleen cells were cultured in RPMI medium with 10% FBS, anti-CD3 and LPS for five days. On day 5, cell cultures were harvested and analyzed by flow cytometry and tabulated into Microsoft Excel. The cell proliferation was presented in relative number and obtained from all living cells. Data were mean ± SD in each group with p value ≤ 0.05

Mentions: Immunocompetent cell labeled with CFSE showed higher fission activity when stimulated with VipAlbumin®in vitro for 5 days. Cells that divide were on the left peak on flow cytometry analysis because it showed a decrease in CFSE luminescence. As shown in Fig. 1, control treatment did not show two different peaks which meant cells in control treatment did not proliferate maximally. Whereas, VipAlbumin® treatment especially D2 and D3 could make two different peaks in FACS result proving that VipAlbumin® was able to increasing cell immunocompetent cell proliferation. Figure 1 also showed that D2 and D3 of VipAlbumin® treatment were able to increase the cell proliferation became 36.67% and 42.28% significantly higher (p < 0.05) than control (28.64%) whereas D1 (33.61%) did not significant (p > 0.05).


The role of VipAlbumin(®) as an immunostimulatory agent for controlling homeostasis and proliferation of lymphoid cells.

Dwijayanti DR, Djati MS, Rifa'I M - Cent Eur J Immunol (2016)

Stimulation cells from spleen by using VipAlbumin® for 5 days showed the increase of cell proliferation relative number. Spleen cells were cultured in RPMI medium with 10% FBS, anti-CD3 and LPS for five days. On day 5, cell cultures were harvested and analyzed by flow cytometry and tabulated into Microsoft Excel. The cell proliferation was presented in relative number and obtained from all living cells. Data were mean ± SD in each group with p value ≤ 0.05
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829819&req=5

Figure 0001: Stimulation cells from spleen by using VipAlbumin® for 5 days showed the increase of cell proliferation relative number. Spleen cells were cultured in RPMI medium with 10% FBS, anti-CD3 and LPS for five days. On day 5, cell cultures were harvested and analyzed by flow cytometry and tabulated into Microsoft Excel. The cell proliferation was presented in relative number and obtained from all living cells. Data were mean ± SD in each group with p value ≤ 0.05
Mentions: Immunocompetent cell labeled with CFSE showed higher fission activity when stimulated with VipAlbumin®in vitro for 5 days. Cells that divide were on the left peak on flow cytometry analysis because it showed a decrease in CFSE luminescence. As shown in Fig. 1, control treatment did not show two different peaks which meant cells in control treatment did not proliferate maximally. Whereas, VipAlbumin® treatment especially D2 and D3 could make two different peaks in FACS result proving that VipAlbumin® was able to increasing cell immunocompetent cell proliferation. Figure 1 also showed that D2 and D3 of VipAlbumin® treatment were able to increase the cell proliferation became 36.67% and 42.28% significantly higher (p < 0.05) than control (28.64%) whereas D1 (33.61%) did not significant (p > 0.05).

Bottom Line: The concentrations of VipAlbumin (®) used were 0 µg/ml; 0.33 µg/ml; 33.3 µg/ml; and 3333.3 µg/ml.VipAlbumin can significantly increase the relative number of CD4(+)CD62L(+) T cell, regulatory T cell, and B220+ cell (p < 0.05) compared to control.This study gives scientific evidence that VipAlbumin can be used as an immunostimulant which accelerates immunocompetent cells growth.

View Article: PubMed Central - PubMed

Affiliation: Biology Department, Brawijaya University, Malang, Indonesia.

ABSTRACT
VipAlbumin(®) is a supplement from snakehead fish (Ophiocephalus striatus) which has high content of albumin that is very important to develop new cells. The aims of this study were to know the effect of VipAlbumin(®) to cell proliferation, expression level of CD4(+)CD62L(+) T cell, regulatory T cell, and B220+ cell, and immunocompetent cell cycle. Cell isolated from spleen of pathogen free mice were cultured in RPMI 1640 with 10% FBS, 1% Pen/Strep 10×, 2-Mercaptoetanol, anti-CD3 and LPS. The concentrations of VipAlbumin (®) used were 0 µg/ml; 0.33 µg/ml; 33.3 µg/ml; and 3333.3 µg/ml. The cell was incubated in CO2 5% incubator 37°C for 3 days for cell cycle and 5 days for proliferation analysis and cell expression. FACS analysis was done to know cell proliferation profile, status of cell, and cell cycle. Concentration 33.3 µg/ml and 3333.3 µg/ml significantly can increase cell proliferation and induce cell enter G2/M phase (p < 0.05) compared to control. VipAlbumin can significantly increase the relative number of CD4(+)CD62L(+) T cell, regulatory T cell, and B220+ cell (p < 0.05) compared to control. This study gives scientific evidence that VipAlbumin can be used as an immunostimulant which accelerates immunocompetent cells growth.

No MeSH data available.


Related in: MedlinePlus