Limits...
Angiomodulatory properties of some antibiotics and Tołpa Peat Preparation.

Radomska-Leśniewska DM, Skopińska-Różewska E, Jóźwiak J, Demkow U, Joanna Bałan B - Cent Eur J Immunol (2016)

Bottom Line: Amikacin did not influence the results of angiogenesis tests.Studied antibiotics (15 mg/kg), except rifamycine, inhibit the angiostimulatory effect of the tested immunomodulator.TPP should be applied after antibiotic therapy to maintain its stimulatory effect and restore proper host immune function.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology, Biostructure Centre, Medical University of Warsaw, Warsaw, Poland.

ABSTRACT

Unlabelled: Deterioration of the immune system due to antibiotic therapy can be restored by immunomodulator application. In this paper we estimate the effect of ampicillin, amikacin, doxycycline, rifampicin, rifamycine and immunomodulator Tołpa Peat Preparation (TPP) on neovascular reaction induced in murine skin by human mononuclear cells (MNC) injection. MNC originating from 15 healthy volunteers were injected intradermally to Balb/c mice. Antibiotics (3, 15, or 75 mg/kg of body weight) alone or with TPP (10 mg/kg of body weight) were administrated subcutaneously to mice on three consecutive days. The number of newly formed blood vessels was measured in dissection microscope 72 hours after cell injection.

Results: TPP stimulated angiogenic activity of MNC at the dose 5 and 10 mg/kg. Rifamycine exerted strong stimulatory action, ampicillin slightly stimulated immune response, while doxycycline and rifampicin downregulated it. Amikacin did not influence the results of angiogenesis tests. Studied antibiotics (15 mg/kg), except rifamycine, inhibit the angiostimulatory effect of the tested immunomodulator. TPP should be applied after antibiotic therapy to maintain its stimulatory effect and restore proper host immune function.

No MeSH data available.


Related in: MedlinePlus

The influence of various doses of Tołpa Peat Preparation (series: OHP G9R) on angiogenic activity of mononuclear cells
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4829815&req=5

Figure 0001: The influence of various doses of Tołpa Peat Preparation (series: OHP G9R) on angiogenic activity of mononuclear cells

Mentions: The results are shown in Figs. 1–5.


Angiomodulatory properties of some antibiotics and Tołpa Peat Preparation.

Radomska-Leśniewska DM, Skopińska-Różewska E, Jóźwiak J, Demkow U, Joanna Bałan B - Cent Eur J Immunol (2016)

The influence of various doses of Tołpa Peat Preparation (series: OHP G9R) on angiogenic activity of mononuclear cells
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829815&req=5

Figure 0001: The influence of various doses of Tołpa Peat Preparation (series: OHP G9R) on angiogenic activity of mononuclear cells
Mentions: The results are shown in Figs. 1–5.

Bottom Line: Amikacin did not influence the results of angiogenesis tests.Studied antibiotics (15 mg/kg), except rifamycine, inhibit the angiostimulatory effect of the tested immunomodulator.TPP should be applied after antibiotic therapy to maintain its stimulatory effect and restore proper host immune function.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology, Biostructure Centre, Medical University of Warsaw, Warsaw, Poland.

ABSTRACT

Unlabelled: Deterioration of the immune system due to antibiotic therapy can be restored by immunomodulator application. In this paper we estimate the effect of ampicillin, amikacin, doxycycline, rifampicin, rifamycine and immunomodulator Tołpa Peat Preparation (TPP) on neovascular reaction induced in murine skin by human mononuclear cells (MNC) injection. MNC originating from 15 healthy volunteers were injected intradermally to Balb/c mice. Antibiotics (3, 15, or 75 mg/kg of body weight) alone or with TPP (10 mg/kg of body weight) were administrated subcutaneously to mice on three consecutive days. The number of newly formed blood vessels was measured in dissection microscope 72 hours after cell injection.

Results: TPP stimulated angiogenic activity of MNC at the dose 5 and 10 mg/kg. Rifamycine exerted strong stimulatory action, ampicillin slightly stimulated immune response, while doxycycline and rifampicin downregulated it. Amikacin did not influence the results of angiogenesis tests. Studied antibiotics (15 mg/kg), except rifamycine, inhibit the angiostimulatory effect of the tested immunomodulator. TPP should be applied after antibiotic therapy to maintain its stimulatory effect and restore proper host immune function.

No MeSH data available.


Related in: MedlinePlus