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In vivo anticancer and immunomodulating activities of mannogalactoglucan-type polysaccharides from Lentinus edodes (Berkeley) Singer.

Jeff IB, Fan E, Tian M, Song C, Yan J, Zhou Y - Cent Eur J Immunol (2016)

Bottom Line: Our results indicated that immunomodulating activity occurred through host mediation in response to lymphocyte proliferation, macrophage phagocytosis and induction of NO production while the antitumor activity occurred through direct cytotoxicity.Our research provides essential data to a better understanding of L. edodes bioactive compounds, especially polysaccharides.Our results also confirm the key role of β-linkages in the antitumor and immunomodulating effects of polysaccharides.

View Article: PubMed Central - PubMed

Affiliation: Northeast Normal University, University of Kinshasa, Changchun, City, Jilin Province, PR China.

ABSTRACT
There is considerable interest in the potential of mushrooms in modulating the immune system and/or suppressing tumor growth. Among the studied bioactive compounds in mushrooms, polysaccharides are the most important. Nontoxic fungal polysaccharides have a more important role in immunomodulating and antitumor activities which are related to their effects to act of immune effecter cells such as lymphocytes, macrophages, dendritic cells, and natural killer cells involved in the innate and adaptive immunity. Two mannogalactoglucan-type polysaccharides (WPLE-N-2 and WPLE-A0.5-2), purified from the fruiting bodies of Lentinus edodes, were evaluated for their effects on the cellular immune response of Sarcoma 180 (S-180)-bearing mice. Mice were treated with 100 mg/kg body weight of the polysaccharides for 10 days. Significant tumor regressions of the polysaccharide groups' mice were observed compared to the control group. These polysaccharides could induce an increase in nitrite oxide (NO) production in peritoneal macrophages, significantly increase macrophage phagocytosis of tumor-bearing mice and augment concanavalin (ConA) and lipopolysaccharide (LPS)-induced splenocytes proliferation. Our results indicated that immunomodulating activity occurred through host mediation in response to lymphocyte proliferation, macrophage phagocytosis and induction of NO production while the antitumor activity occurred through direct cytotoxicity. Our findings suggest that mannogalactoglucan-type polysaccharides from L. edodes can be explored as novel potential immunostimulants. Our research provides essential data to a better understanding of L. edodes bioactive compounds, especially polysaccharides. Our results also confirm the key role of β-linkages in the antitumor and immunomodulating effects of polysaccharides.

No MeSH data available.


Related in: MedlinePlus

Effect of the mannogalactoglucan-type polysaccharides WPLE-N-2 and WPLE-A0.5-2 on NO production in macrophages. Values are means ± SD of 6 mice; *p < 0.05 and **p < 0.01 vs. control
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Figure 0003: Effect of the mannogalactoglucan-type polysaccharides WPLE-N-2 and WPLE-A0.5-2 on NO production in macrophages. Values are means ± SD of 6 mice; *p < 0.05 and **p < 0.01 vs. control

Mentions: Nitrite oxide is one of the major reactive oxygen species (ROS) which is produced by macrophage for destroying the foreign body. Since the final product of NO is either nitrite or nitrate, the sum of nitrite and nitrate level provides an indirect measurement of NO level. Macrophages were incubated with mannogalactoglucan-type polysaccharides for 24 hours and NO concentrations in the culture supernatants were assessed by the Griess reagent. The polysaccharides presented a higher effect on NO production at the dose of 100 mg/kg body weight when compared with the control. The effect of WPLE-A0.5-2 was slightly higher than that of WPLE-N-2, but the difference was not significant as shown in Fig. 3.


In vivo anticancer and immunomodulating activities of mannogalactoglucan-type polysaccharides from Lentinus edodes (Berkeley) Singer.

Jeff IB, Fan E, Tian M, Song C, Yan J, Zhou Y - Cent Eur J Immunol (2016)

Effect of the mannogalactoglucan-type polysaccharides WPLE-N-2 and WPLE-A0.5-2 on NO production in macrophages. Values are means ± SD of 6 mice; *p < 0.05 and **p < 0.01 vs. control
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829809&req=5

Figure 0003: Effect of the mannogalactoglucan-type polysaccharides WPLE-N-2 and WPLE-A0.5-2 on NO production in macrophages. Values are means ± SD of 6 mice; *p < 0.05 and **p < 0.01 vs. control
Mentions: Nitrite oxide is one of the major reactive oxygen species (ROS) which is produced by macrophage for destroying the foreign body. Since the final product of NO is either nitrite or nitrate, the sum of nitrite and nitrate level provides an indirect measurement of NO level. Macrophages were incubated with mannogalactoglucan-type polysaccharides for 24 hours and NO concentrations in the culture supernatants were assessed by the Griess reagent. The polysaccharides presented a higher effect on NO production at the dose of 100 mg/kg body weight when compared with the control. The effect of WPLE-A0.5-2 was slightly higher than that of WPLE-N-2, but the difference was not significant as shown in Fig. 3.

Bottom Line: Our results indicated that immunomodulating activity occurred through host mediation in response to lymphocyte proliferation, macrophage phagocytosis and induction of NO production while the antitumor activity occurred through direct cytotoxicity.Our research provides essential data to a better understanding of L. edodes bioactive compounds, especially polysaccharides.Our results also confirm the key role of β-linkages in the antitumor and immunomodulating effects of polysaccharides.

View Article: PubMed Central - PubMed

Affiliation: Northeast Normal University, University of Kinshasa, Changchun, City, Jilin Province, PR China.

ABSTRACT
There is considerable interest in the potential of mushrooms in modulating the immune system and/or suppressing tumor growth. Among the studied bioactive compounds in mushrooms, polysaccharides are the most important. Nontoxic fungal polysaccharides have a more important role in immunomodulating and antitumor activities which are related to their effects to act of immune effecter cells such as lymphocytes, macrophages, dendritic cells, and natural killer cells involved in the innate and adaptive immunity. Two mannogalactoglucan-type polysaccharides (WPLE-N-2 and WPLE-A0.5-2), purified from the fruiting bodies of Lentinus edodes, were evaluated for their effects on the cellular immune response of Sarcoma 180 (S-180)-bearing mice. Mice were treated with 100 mg/kg body weight of the polysaccharides for 10 days. Significant tumor regressions of the polysaccharide groups' mice were observed compared to the control group. These polysaccharides could induce an increase in nitrite oxide (NO) production in peritoneal macrophages, significantly increase macrophage phagocytosis of tumor-bearing mice and augment concanavalin (ConA) and lipopolysaccharide (LPS)-induced splenocytes proliferation. Our results indicated that immunomodulating activity occurred through host mediation in response to lymphocyte proliferation, macrophage phagocytosis and induction of NO production while the antitumor activity occurred through direct cytotoxicity. Our findings suggest that mannogalactoglucan-type polysaccharides from L. edodes can be explored as novel potential immunostimulants. Our research provides essential data to a better understanding of L. edodes bioactive compounds, especially polysaccharides. Our results also confirm the key role of β-linkages in the antitumor and immunomodulating effects of polysaccharides.

No MeSH data available.


Related in: MedlinePlus