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Identification and Expression Analysis of BURP Domain-Containing Genes in Medicago truncatula.

Li Y, Chen X, Chen Z, Cai R, Zhang H, Xiang Y - Front Plant Sci (2016)

Bottom Line: BURP domain-containing proteins belong to a newly identified protein class that is unique to plants and plays an important role in plant development and metabolism.These genes have diverse structures and were distributed on chromosome 1-8 except 7.Motif and exon-intron organization, stress-related cis-elements in promoter regions and microarray analysis of MtBURPs were also performed.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Modern Biotechnology, School of Forestry and Landscape Architecture, Anhui Agricultural University Hefei, China.

ABSTRACT
BURP domain-containing proteins belong to a newly identified protein class that is unique to plants and plays an important role in plant development and metabolism. Although systematic characterization of BURP domain-containing proteins have been carried out in many species, such as rice, poplar and maize, little is known about BURP domain-containing proteins in Medicago. In this study, multiple bioinformatics approaches were employed to identify all the members of BURP family genes in Medicago. A complete set of 39 BURP family genes were identified. These genes have diverse structures and were distributed on chromosome 1-8 except 7. According to phylogenetic analysis, these BURP family genes could be classified into eight classes. Motif and exon-intron organization, stress-related cis-elements in promoter regions and microarray analysis of MtBURPs were also performed. Furthermore, transcript level analysis of MtBURP genes in response to drought stress revealed that all of the 39 BURP genes were regulated by drought stress. The results of this study reveal a comprehensive overview of the Medicago BURP gene family and provide the first step toward the selection of MtBURP genes for cloning and functional analysis of the BURP gene family in Medicago truncatula.

No MeSH data available.


Related in: MedlinePlus

The structure of exon-introns in MtBURP genes. Exons, intron and UTRs are indicated with different colored lines. The length of each MtBURP gene can be estimated using the scale at the bottom of the figure.
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Figure 3: The structure of exon-introns in MtBURP genes. Exons, intron and UTRs are indicated with different colored lines. The length of each MtBURP gene can be estimated using the scale at the bottom of the figure.

Mentions: The exon-intron structural analysis of the MtBURP genes was also performed by the authors. Each CDS of the MtBURP genes is disrupted by one or more exons (Figure 3), indicating that the BURP protein diversity. The number of exons in each gene varies widely among the MtBURP genes. The largest number is 4, such as MtBURP31, -36 and -39. By contrast, the MtBURP38, -18 and -34 contain only one exon.


Identification and Expression Analysis of BURP Domain-Containing Genes in Medicago truncatula.

Li Y, Chen X, Chen Z, Cai R, Zhang H, Xiang Y - Front Plant Sci (2016)

The structure of exon-introns in MtBURP genes. Exons, intron and UTRs are indicated with different colored lines. The length of each MtBURP gene can be estimated using the scale at the bottom of the figure.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829796&req=5

Figure 3: The structure of exon-introns in MtBURP genes. Exons, intron and UTRs are indicated with different colored lines. The length of each MtBURP gene can be estimated using the scale at the bottom of the figure.
Mentions: The exon-intron structural analysis of the MtBURP genes was also performed by the authors. Each CDS of the MtBURP genes is disrupted by one or more exons (Figure 3), indicating that the BURP protein diversity. The number of exons in each gene varies widely among the MtBURP genes. The largest number is 4, such as MtBURP31, -36 and -39. By contrast, the MtBURP38, -18 and -34 contain only one exon.

Bottom Line: BURP domain-containing proteins belong to a newly identified protein class that is unique to plants and plays an important role in plant development and metabolism.These genes have diverse structures and were distributed on chromosome 1-8 except 7.Motif and exon-intron organization, stress-related cis-elements in promoter regions and microarray analysis of MtBURPs were also performed.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Modern Biotechnology, School of Forestry and Landscape Architecture, Anhui Agricultural University Hefei, China.

ABSTRACT
BURP domain-containing proteins belong to a newly identified protein class that is unique to plants and plays an important role in plant development and metabolism. Although systematic characterization of BURP domain-containing proteins have been carried out in many species, such as rice, poplar and maize, little is known about BURP domain-containing proteins in Medicago. In this study, multiple bioinformatics approaches were employed to identify all the members of BURP family genes in Medicago. A complete set of 39 BURP family genes were identified. These genes have diverse structures and were distributed on chromosome 1-8 except 7. According to phylogenetic analysis, these BURP family genes could be classified into eight classes. Motif and exon-intron organization, stress-related cis-elements in promoter regions and microarray analysis of MtBURPs were also performed. Furthermore, transcript level analysis of MtBURP genes in response to drought stress revealed that all of the 39 BURP genes were regulated by drought stress. The results of this study reveal a comprehensive overview of the Medicago BURP gene family and provide the first step toward the selection of MtBURP genes for cloning and functional analysis of the BURP gene family in Medicago truncatula.

No MeSH data available.


Related in: MedlinePlus