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Bioactive Components of Chinese Propolis Water Extract on Antitumor Activity and Quality Control.

Xuan H, Wang Y, Li A, Fu C, Wang Y, Peng W - Evid Based Complement Alternat Med (2016)

Bottom Line: Furthermore, cell migration, procaspase 3 level, and reactive oxygen species (ROS) of effective components from CPWE were investigated.CPWE and its effective components induced apoptosis by inhibiting tumor cell migration, activating caspase 3, and promoting ROS production.It can be deduced that the antitumor effects of propolis did not depend on a single component, and there must exist "bioactive components," which also provides a new idea for Chinese propolis quality control.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Beijing 100093, China; School of Life Science, Liaocheng University, Liaocheng 252059, China.

ABSTRACT
To understand the material basis of antitumor activity of Chinese propolis water extract (CPWE), we developed a simple and efficient method using macroporous absorptive resin coupled with preparative high performance liquid chromatography and separated and purified eleven chemical components (caffeic acid, ferulic acid, isoferulic acid, 3,4-dimethoxycinnamic acid, pinobanksin, caffeic acid benzyl ester, caffeic acid phenethyl ester, apigenin, pinocembrin, chrysin, and galangin) from CPWE; then we tested the antitumor activities of these eleven components using different human tumor cell lines (MCF-7, MDA-MB-231, HeLa, and A549). Furthermore, cell migration, procaspase 3 level, and reactive oxygen species (ROS) of effective components from CPWE were investigated. Our data showed that antitumor activities of the eleven components from CPWE were different from each other. CPWE and its effective components induced apoptosis by inhibiting tumor cell migration, activating caspase 3, and promoting ROS production. It can be deduced that the antitumor effects of propolis did not depend on a single component, and there must exist "bioactive components," which also provides a new idea for Chinese propolis quality control.

No MeSH data available.


Related in: MedlinePlus

Effects of the seven components and crude CPWE on migration of MDA-MB-231 cells. (a) Cell migration micrographs obtained under a phase contrast microscope at 0 and 48 h (×100). (b) Relative levels of cell migration (∗P < 0.05, ∗∗P < 0.01 versus control, n = 3).
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fig4: Effects of the seven components and crude CPWE on migration of MDA-MB-231 cells. (a) Cell migration micrographs obtained under a phase contrast microscope at 0 and 48 h (×100). (b) Relative levels of cell migration (∗P < 0.05, ∗∗P < 0.01 versus control, n = 3).

Mentions: The migrations of MDA-MB-231 cells were detected by wound-healing assay after being treated with the seven different components at concentration of 80 μM and CPWE (100 μg/mL); the results indicated that the seven different components and CPWE significantly inhibited MDA-MB-231 cells migration at 48 h (Figure 4).


Bioactive Components of Chinese Propolis Water Extract on Antitumor Activity and Quality Control.

Xuan H, Wang Y, Li A, Fu C, Wang Y, Peng W - Evid Based Complement Alternat Med (2016)

Effects of the seven components and crude CPWE on migration of MDA-MB-231 cells. (a) Cell migration micrographs obtained under a phase contrast microscope at 0 and 48 h (×100). (b) Relative levels of cell migration (∗P < 0.05, ∗∗P < 0.01 versus control, n = 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4829702&req=5

fig4: Effects of the seven components and crude CPWE on migration of MDA-MB-231 cells. (a) Cell migration micrographs obtained under a phase contrast microscope at 0 and 48 h (×100). (b) Relative levels of cell migration (∗P < 0.05, ∗∗P < 0.01 versus control, n = 3).
Mentions: The migrations of MDA-MB-231 cells were detected by wound-healing assay after being treated with the seven different components at concentration of 80 μM and CPWE (100 μg/mL); the results indicated that the seven different components and CPWE significantly inhibited MDA-MB-231 cells migration at 48 h (Figure 4).

Bottom Line: Furthermore, cell migration, procaspase 3 level, and reactive oxygen species (ROS) of effective components from CPWE were investigated.CPWE and its effective components induced apoptosis by inhibiting tumor cell migration, activating caspase 3, and promoting ROS production.It can be deduced that the antitumor effects of propolis did not depend on a single component, and there must exist "bioactive components," which also provides a new idea for Chinese propolis quality control.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Beijing 100093, China; School of Life Science, Liaocheng University, Liaocheng 252059, China.

ABSTRACT
To understand the material basis of antitumor activity of Chinese propolis water extract (CPWE), we developed a simple and efficient method using macroporous absorptive resin coupled with preparative high performance liquid chromatography and separated and purified eleven chemical components (caffeic acid, ferulic acid, isoferulic acid, 3,4-dimethoxycinnamic acid, pinobanksin, caffeic acid benzyl ester, caffeic acid phenethyl ester, apigenin, pinocembrin, chrysin, and galangin) from CPWE; then we tested the antitumor activities of these eleven components using different human tumor cell lines (MCF-7, MDA-MB-231, HeLa, and A549). Furthermore, cell migration, procaspase 3 level, and reactive oxygen species (ROS) of effective components from CPWE were investigated. Our data showed that antitumor activities of the eleven components from CPWE were different from each other. CPWE and its effective components induced apoptosis by inhibiting tumor cell migration, activating caspase 3, and promoting ROS production. It can be deduced that the antitumor effects of propolis did not depend on a single component, and there must exist "bioactive components," which also provides a new idea for Chinese propolis quality control.

No MeSH data available.


Related in: MedlinePlus