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Comparison of Calcium and Barium Microcapsules as Scaffolds in the Development of Artificial Dermal Papillae.

Liu Y, Lin C, Zeng Y, Li H, Cai B, Huang K, Yuan Y, Li Y - Biomed Res Int (2016)

Bottom Line: A few large hair follicles were found.Barium microcapsules were superior to calcium microcapsules in structural and mechanical stability.Calcium microcapsules may provide an effective scaffold for the development of artificial dermal papilla.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology, Shantou University Medical College, Shantou, Guangdong 515000, China.

ABSTRACT
This study aimed to develop and evaluate barium and calcium microcapsules as candidates for scaffolding in artificial dermal papilla. Dermal papilla cells (DPCs) were isolated and cultured by one-step collagenase treatment. The DPC-Ba and DPC-Ca microcapsules were prepared by using a specially designed, high-voltage, electric-field droplet generator. Selected microcapsules were assessed for long-term inductive properties with xenotransplantation into Sprague-Dawley rat ears. Both barium and calcium microcapsules maintained xenogenic dermal papilla cells in an immunoisolated environment and induced the formation of hair follicle structures. Calcium microcapsules showed better biocompatibility, permeability, and cell viability in comparison with barium microcapsules. Before 18 weeks, calcium microcapsules gathered together, with no substantial immune response. After 32 weeks, some microcapsules were near inflammatory cells and wrapped with fiber. A few large hair follicles were found. Control samples showed no marked changes at the implantation site. Barium microcapsules were superior to calcium microcapsules in structural and mechanical stability. The cells encapsulated in hydrogel barium microcapsules exhibited higher short-term viability. This study established a model to culture DPCs in 3D culture conditions. Barium microcapsules may be useful in short-term transplantation study. Calcium microcapsules may provide an effective scaffold for the development of artificial dermal papilla.

No MeSH data available.


Related in: MedlinePlus

Short-term study of DPC-Ca and DPC-Ba microcapsules transplanted into rat ears. Large hair follicles formed after Ca microcapsule implantation at weeks 1–4 (a, b, e, f, i, j, m, and n). At 2 weeks, large DP formed near the transplanted site without the surrounding DPC microcapsule (e, f). No abnormal hair follicle structures were found in Ba microcapsules at 1 to 2 weeks after transplantation (c, d, g, and h). At 3 to 4 weeks, Ba (k, l, o, and p) microcapsules produced large hair follicles but fewer compared with Ca (i, j, m, and n) microcapsules. (Bar = 100 μm.)
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fig6: Short-term study of DPC-Ca and DPC-Ba microcapsules transplanted into rat ears. Large hair follicles formed after Ca microcapsule implantation at weeks 1–4 (a, b, e, f, i, j, m, and n). At 2 weeks, large DP formed near the transplanted site without the surrounding DPC microcapsule (e, f). No abnormal hair follicle structures were found in Ba microcapsules at 1 to 2 weeks after transplantation (c, d, g, and h). At 3 to 4 weeks, Ba (k, l, o, and p) microcapsules produced large hair follicles but fewer compared with Ca (i, j, m, and n) microcapsules. (Bar = 100 μm.)

Mentions: When implanted into hairless regions of SD rat ears, both DPC-Ba and DPC-Ca microcapsules induced the de novo growth of a large number of HFs in rat ears within 2 to 4 weeks. When compared with Ba microcapsules, Ca microcapsules showed stronger induction. The induced HF number, size, and visible fibers were significantly greater with Ca microcapsules compared with Ba microcapsules (Table 1). The number and size of abnormal HF and sebaceous glands were greater with Ca microcapsules compared with the Ba microcapsules (Figure 6). The new large HFs in case of Ca microcapsules showed the basic morphological features of human HFs and visible hair fibers at 6 weeks. The longest fibers grew to 7 to 8 mm. These abnormal fibers remained for 3 to 4 weeks and then fell off. Also the large HFs in case of Ca microcapsules remained until 8 weeks and reduced gradually in number but were absent in Ba microcapsules within 2 to 3 weeks. While the inflammatory cells gathered and blood vessels proliferated at the injected sites during the first week following the transplantation for the Ba microcapsules, the Ca microcapsules did not produce these reactions until 24 weeks.


Comparison of Calcium and Barium Microcapsules as Scaffolds in the Development of Artificial Dermal Papillae.

Liu Y, Lin C, Zeng Y, Li H, Cai B, Huang K, Yuan Y, Li Y - Biomed Res Int (2016)

Short-term study of DPC-Ca and DPC-Ba microcapsules transplanted into rat ears. Large hair follicles formed after Ca microcapsule implantation at weeks 1–4 (a, b, e, f, i, j, m, and n). At 2 weeks, large DP formed near the transplanted site without the surrounding DPC microcapsule (e, f). No abnormal hair follicle structures were found in Ba microcapsules at 1 to 2 weeks after transplantation (c, d, g, and h). At 3 to 4 weeks, Ba (k, l, o, and p) microcapsules produced large hair follicles but fewer compared with Ca (i, j, m, and n) microcapsules. (Bar = 100 μm.)
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4829698&req=5

fig6: Short-term study of DPC-Ca and DPC-Ba microcapsules transplanted into rat ears. Large hair follicles formed after Ca microcapsule implantation at weeks 1–4 (a, b, e, f, i, j, m, and n). At 2 weeks, large DP formed near the transplanted site without the surrounding DPC microcapsule (e, f). No abnormal hair follicle structures were found in Ba microcapsules at 1 to 2 weeks after transplantation (c, d, g, and h). At 3 to 4 weeks, Ba (k, l, o, and p) microcapsules produced large hair follicles but fewer compared with Ca (i, j, m, and n) microcapsules. (Bar = 100 μm.)
Mentions: When implanted into hairless regions of SD rat ears, both DPC-Ba and DPC-Ca microcapsules induced the de novo growth of a large number of HFs in rat ears within 2 to 4 weeks. When compared with Ba microcapsules, Ca microcapsules showed stronger induction. The induced HF number, size, and visible fibers were significantly greater with Ca microcapsules compared with Ba microcapsules (Table 1). The number and size of abnormal HF and sebaceous glands were greater with Ca microcapsules compared with the Ba microcapsules (Figure 6). The new large HFs in case of Ca microcapsules showed the basic morphological features of human HFs and visible hair fibers at 6 weeks. The longest fibers grew to 7 to 8 mm. These abnormal fibers remained for 3 to 4 weeks and then fell off. Also the large HFs in case of Ca microcapsules remained until 8 weeks and reduced gradually in number but were absent in Ba microcapsules within 2 to 3 weeks. While the inflammatory cells gathered and blood vessels proliferated at the injected sites during the first week following the transplantation for the Ba microcapsules, the Ca microcapsules did not produce these reactions until 24 weeks.

Bottom Line: A few large hair follicles were found.Barium microcapsules were superior to calcium microcapsules in structural and mechanical stability.Calcium microcapsules may provide an effective scaffold for the development of artificial dermal papilla.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology, Shantou University Medical College, Shantou, Guangdong 515000, China.

ABSTRACT
This study aimed to develop and evaluate barium and calcium microcapsules as candidates for scaffolding in artificial dermal papilla. Dermal papilla cells (DPCs) were isolated and cultured by one-step collagenase treatment. The DPC-Ba and DPC-Ca microcapsules were prepared by using a specially designed, high-voltage, electric-field droplet generator. Selected microcapsules were assessed for long-term inductive properties with xenotransplantation into Sprague-Dawley rat ears. Both barium and calcium microcapsules maintained xenogenic dermal papilla cells in an immunoisolated environment and induced the formation of hair follicle structures. Calcium microcapsules showed better biocompatibility, permeability, and cell viability in comparison with barium microcapsules. Before 18 weeks, calcium microcapsules gathered together, with no substantial immune response. After 32 weeks, some microcapsules were near inflammatory cells and wrapped with fiber. A few large hair follicles were found. Control samples showed no marked changes at the implantation site. Barium microcapsules were superior to calcium microcapsules in structural and mechanical stability. The cells encapsulated in hydrogel barium microcapsules exhibited higher short-term viability. This study established a model to culture DPCs in 3D culture conditions. Barium microcapsules may be useful in short-term transplantation study. Calcium microcapsules may provide an effective scaffold for the development of artificial dermal papilla.

No MeSH data available.


Related in: MedlinePlus