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Comparison of Calcium and Barium Microcapsules as Scaffolds in the Development of Artificial Dermal Papillae.

Liu Y, Lin C, Zeng Y, Li H, Cai B, Huang K, Yuan Y, Li Y - Biomed Res Int (2016)

Bottom Line: A few large hair follicles were found.Barium microcapsules were superior to calcium microcapsules in structural and mechanical stability.Calcium microcapsules may provide an effective scaffold for the development of artificial dermal papilla.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology, Shantou University Medical College, Shantou, Guangdong 515000, China.

ABSTRACT
This study aimed to develop and evaluate barium and calcium microcapsules as candidates for scaffolding in artificial dermal papilla. Dermal papilla cells (DPCs) were isolated and cultured by one-step collagenase treatment. The DPC-Ba and DPC-Ca microcapsules were prepared by using a specially designed, high-voltage, electric-field droplet generator. Selected microcapsules were assessed for long-term inductive properties with xenotransplantation into Sprague-Dawley rat ears. Both barium and calcium microcapsules maintained xenogenic dermal papilla cells in an immunoisolated environment and induced the formation of hair follicle structures. Calcium microcapsules showed better biocompatibility, permeability, and cell viability in comparison with barium microcapsules. Before 18 weeks, calcium microcapsules gathered together, with no substantial immune response. After 32 weeks, some microcapsules were near inflammatory cells and wrapped with fiber. A few large hair follicles were found. Control samples showed no marked changes at the implantation site. Barium microcapsules were superior to calcium microcapsules in structural and mechanical stability. The cells encapsulated in hydrogel barium microcapsules exhibited higher short-term viability. This study established a model to culture DPCs in 3D culture conditions. Barium microcapsules may be useful in short-term transplantation study. Calcium microcapsules may provide an effective scaffold for the development of artificial dermal papilla.

No MeSH data available.


Related in: MedlinePlus

Structural stability microcapsules. Thousands each of DPC-Ba and DPC-Ca microcapsules were assessed by determining change in structural integrity under an inverted microscope at 10 and 60 min after being stirred in flask (a). Cells inside the fragmented Ca microcapsules escaped (b). Cells were retained inside the membrane of Ba microcapsules, even after fragmentation (c). 1 mL of Ba and Ca microcapsules of 400 μm diameter were injected through 7#, 9#, and 16# pinheads, and integrated microcapsules were counted under a phase-contrast microscope (d). (Bar = 100 μm.)
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fig3: Structural stability microcapsules. Thousands each of DPC-Ba and DPC-Ca microcapsules were assessed by determining change in structural integrity under an inverted microscope at 10 and 60 min after being stirred in flask (a). Cells inside the fragmented Ca microcapsules escaped (b). Cells were retained inside the membrane of Ba microcapsules, even after fragmentation (c). 1 mL of Ba and Ca microcapsules of 400 μm diameter were injected through 7#, 9#, and 16# pinheads, and integrated microcapsules were counted under a phase-contrast microscope (d). (Bar = 100 μm.)

Mentions: The structural stability was significantly higher for Ba compared with Ca microcapsules during whisking and injection. The numbers of Ba and Ca microcapsules that retained structural integrity at 10 min were 84.8% and 73.2%, respectively (P < 0.05). At 60 min, their numbers were 36.3% and 12.0%, respectively (P < 0.01) (Figure 3(a)). The contents inside the Ca microcapsules escaped when fragmented (Figure 3(b)). However, the cells were retained within the membrane for Ba microcapsules, even after fragmentation (Figure 3(c)).


Comparison of Calcium and Barium Microcapsules as Scaffolds in the Development of Artificial Dermal Papillae.

Liu Y, Lin C, Zeng Y, Li H, Cai B, Huang K, Yuan Y, Li Y - Biomed Res Int (2016)

Structural stability microcapsules. Thousands each of DPC-Ba and DPC-Ca microcapsules were assessed by determining change in structural integrity under an inverted microscope at 10 and 60 min after being stirred in flask (a). Cells inside the fragmented Ca microcapsules escaped (b). Cells were retained inside the membrane of Ba microcapsules, even after fragmentation (c). 1 mL of Ba and Ca microcapsules of 400 μm diameter were injected through 7#, 9#, and 16# pinheads, and integrated microcapsules were counted under a phase-contrast microscope (d). (Bar = 100 μm.)
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4829698&req=5

fig3: Structural stability microcapsules. Thousands each of DPC-Ba and DPC-Ca microcapsules were assessed by determining change in structural integrity under an inverted microscope at 10 and 60 min after being stirred in flask (a). Cells inside the fragmented Ca microcapsules escaped (b). Cells were retained inside the membrane of Ba microcapsules, even after fragmentation (c). 1 mL of Ba and Ca microcapsules of 400 μm diameter were injected through 7#, 9#, and 16# pinheads, and integrated microcapsules were counted under a phase-contrast microscope (d). (Bar = 100 μm.)
Mentions: The structural stability was significantly higher for Ba compared with Ca microcapsules during whisking and injection. The numbers of Ba and Ca microcapsules that retained structural integrity at 10 min were 84.8% and 73.2%, respectively (P < 0.05). At 60 min, their numbers were 36.3% and 12.0%, respectively (P < 0.01) (Figure 3(a)). The contents inside the Ca microcapsules escaped when fragmented (Figure 3(b)). However, the cells were retained within the membrane for Ba microcapsules, even after fragmentation (Figure 3(c)).

Bottom Line: A few large hair follicles were found.Barium microcapsules were superior to calcium microcapsules in structural and mechanical stability.Calcium microcapsules may provide an effective scaffold for the development of artificial dermal papilla.

View Article: PubMed Central - PubMed

Affiliation: Department of Histology and Embryology, Shantou University Medical College, Shantou, Guangdong 515000, China.

ABSTRACT
This study aimed to develop and evaluate barium and calcium microcapsules as candidates for scaffolding in artificial dermal papilla. Dermal papilla cells (DPCs) were isolated and cultured by one-step collagenase treatment. The DPC-Ba and DPC-Ca microcapsules were prepared by using a specially designed, high-voltage, electric-field droplet generator. Selected microcapsules were assessed for long-term inductive properties with xenotransplantation into Sprague-Dawley rat ears. Both barium and calcium microcapsules maintained xenogenic dermal papilla cells in an immunoisolated environment and induced the formation of hair follicle structures. Calcium microcapsules showed better biocompatibility, permeability, and cell viability in comparison with barium microcapsules. Before 18 weeks, calcium microcapsules gathered together, with no substantial immune response. After 32 weeks, some microcapsules were near inflammatory cells and wrapped with fiber. A few large hair follicles were found. Control samples showed no marked changes at the implantation site. Barium microcapsules were superior to calcium microcapsules in structural and mechanical stability. The cells encapsulated in hydrogel barium microcapsules exhibited higher short-term viability. This study established a model to culture DPCs in 3D culture conditions. Barium microcapsules may be useful in short-term transplantation study. Calcium microcapsules may provide an effective scaffold for the development of artificial dermal papilla.

No MeSH data available.


Related in: MedlinePlus